Neutralizing Ljungan virus antibodies in children with newly diagnosed type 1 diabetes

2021 ◽  
Vol 102 (5) ◽  
Author(s):  
Annika Lundstig ◽  
Sharia L. McDonald ◽  
Marlena Maziarz ◽  
William C. Weldon ◽  
Fariba Vaziri-Sani ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Bank Vole ◽  
Acid Decarboxylase ◽  
Newly Diagnosed ◽  
Future Studies ◽  
Ljungan Virus ◽  
Islet Antigen ◽  
Diabetes Patients

Ljungan virus (LV), a Parechovirus of the Picornavirus family, first isolated from a bank vole at the Ljungan river in Sweden, has been implicated in the risk for autoimmune type 1 diabetes. An assay for neutralizing Ljungan virus antibodies (NLVA) was developed using the original 87–012 LV isolate. The goal was to determine NLVA titres in incident 0–18 years old newly diagnosed type 1 diabetes patients (n=67) and school children controls (n=292) from Jämtland county in Sweden. NLVA were found in 41 of 67 (61 %) patients compared to 127 of 292 (44 %) controls (P=0.009). In the type 1 diabetes patients, NLVA titres were associated with autoantibodies to glutamic acid decarboxylase (GADA) (P=0.023), but not to autoantibodies against insulin (IAA) or islet antigen-2 (IA-2A). The NLVA assay should prove useful for further investigations to determine levels of LV antibodies in patients and future studies to determine a possible role of LV in autoimmune type 1 diabetes.

scholarly journals Increased DKA at presentation among newly diagnosed type 1 diabetes patients with or without COVID ‐19: Data from a multi‐site surveillance registry

2020 ◽  
Author(s):  
Kara Beliard ◽  
Osagie Ebekozien ◽  
Carla Demeterco‐Berggren ◽  
Guy Todd Alonso ◽  
Mary Pat Gallagher ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Newly Diagnosed ◽  
Diabetes Patients

scholarly journals A study of 51 subtypes of peripheral blood immune cells in newly diagnosed young type 1 diabetes patients

2019 ◽  
Vol 198 (1) ◽  
pp. 57-70 ◽  
Author(s):  
A. Oras ◽  
A. Peet ◽  
T. Giese ◽  
V. Tillmann ◽  
R. Uibo
Keyword(s):  
Type 1 Diabetes ◽  
Immune Cells ◽  
Peripheral Blood ◽  
Newly Diagnosed ◽  
Diabetes Patients

The role of cubilin gene polymorphisms and their influence on 25(OH)D3 and 1,25(OH)2D3 plasma levels in type 1 diabetes patients

2010 ◽  
Vol 121 (1-2) ◽  
pp. 442-444 ◽  
Author(s):  
Elizabeth Ramos-Lopez ◽  
Brita Lange ◽  
Marissa Penna-Martinez ◽  
Patrick Brück ◽  
Karolina Swiech ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Plasma Levels ◽  
Gene Polymorphisms ◽  
Diabetes Patients

Newly diagnosed type 1 diabetes patients exhibit lower hepatic ATP concentrations despite normal hepatocellular lipid accumulation

2015 ◽  
Vol 10 (S 01) ◽  
Author(s):  
S Gancheva ◽  
A Bierwagen ◽  
P Begovatz ◽  
J Lundbom ◽  
P Nowotny ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Lipid Accumulation ◽  
Newly Diagnosed ◽  
Diabetes Patients

scholarly journals Human Mesenchymal Stem Cells Modulate Cellular Immune Response to Islet Antigen Glutamic Acid Decarboxylase in Type 1 Diabetes

2010 ◽  
Vol 95 (8) ◽  
pp. 3788-3797 ◽  
Author(s):  
Maria M. Zanone ◽  
Enrica Favaro ◽  
Ilaria Miceli ◽  
Giorgio Grassi ◽  
Elisa Camussi ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Acid Decarboxylase ◽  
Diabetic Patients ◽  
Human Mscs ◽  
T Helper ◽  
Ifn Γ ◽  
Islet Antigen ◽  
Helper Type

Context: Mesenchymal stem cells (MSCs) exert an immunosuppressive effect on the immune system. However, studies on the immunomodulatory potential of MSCs in type 1 diabetes are lacking. Objective: We aimed to evaluate whether human MSCs may inhibit in vitro pancreatic islet antigen-specific T cell activation in type 1 diabetes. Design: Human MSCs were isolated and characterized. Peripheral blood mononuclear cells (PBMCs) were obtained from nine type 1 diabetic patients at disease onset and 13 healthy control subjects. IFN-γ, IL-10, and IL-4 enzyme-linked immunospot responses of lymphocytes incubated with glutamic acid decarboxylase 65 (GAD65) were investigated in PBMC cultures and PBMC/MSC cocultures. Levels of prostaglandin E2 (PGE2), IFN-γ, IL-4, and IL-10 in supernatants were measured by ELISA. PGE2 inhibition experiments with NS-398 and indomethacin were also performed. Results: Five diabetic patients were identified with a positive PBMC IFN-γ response to GAD65 and negative IL-10 and IL-4 response. PBMC/MSC cocultures resulted in a significant decrease in the number of spots and in detection of IL-4-secreting cells. PGE2 inhibitors abrogated the immune-suppressive effect, indicating an involvement of PGE2 production, and the constitutive production of PGE2 by MSCs was enhanced in PBMC/MSC coculture. Moreover, in GAD-responder patients, GAD-stimulated PBMC/MSC cocultures significantly decreased secretion of IFN-γ and IL-10 and increased secretion of IL-4. Conclusions: These results provide evidence that human MSCs abrogate in vitro a proinflammatory T helper type 1 response to an islet antigenic stimulus in type 1 diabetes. MSCs induce IL-4-producing cells, suggesting a possible switch to an antiinflammatory T helper type 2 signaling of T cells.

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