Bacterial glycoproteomics

Glycosylated proteins are ubiquitous components of eukaryote cellular surfaces, where the glycan moieties are implicated in a wide range of cell–cell recognition events. Once thought to be restricted to eukaryotes, glycosylation is now being increasingly reported in prokaryotes. Many of these discoveries have grown from advances in analytical technologies and genome sequencing. This review highlights the capabilities of high-sensitivity mass spectrometry for carbohydrate structure determination of bacterial glycoproteins and the emergence of glycoproteomic strategies that have evolved from proteomics and genomics for the functional analysis of bacterial glycosylation.

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Simultaneous determination of volatile organic compounds with a wide range of polarities in urine by headspace solid-phase microextraction coupled to gas chromatography/mass spectrometry

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.7827 ◽

2017 ◽

Vol 31 (7) ◽

pp. 613-622 ◽

Cited By ~ 10

Author(s):

Han-Na Song ◽

Chong Hyeak Kim ◽

Won-Yong Lee ◽

Sung-Hee Cho

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Volatile Organic Compounds ◽

Organic Compounds ◽

Solid Phase Microextraction ◽

Solid Phase ◽

Gas Chromatography Mass Spectrometry ◽

Wide Range ◽

Volatile Organic

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Mass Spectrometry: Structure Determination of Proteins and Peptides☆

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering ◽

10.1016/b978-0-12-409547-2.12667-8 ◽

2016 ◽

Author(s):

J. Zoidakis ◽

D. Chatziharalambous

Keyword(s):

Mass Spectrometry ◽

Structure Determination ◽

Proteins And Peptides

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Copper-nickel oxide nanofilm modified electrode for non-enzymatic determination of glucose

Journal of Electrochemical Science and Engineering ◽

10.5599/jese.699 ◽

2020 ◽

Vol 10 (3) ◽

pp. 245-255

Author(s):

Mahsa Hasanzadeh ◽

Zahra Hasanzadeh ◽

Sakineh Alizadeh ◽

Mehran Sayadi ◽

Mojtaba Nasiri Nezhad ◽

...

Keyword(s):

Modified Electrode ◽

Limit Of Detection ◽

High Sensitivity ◽

Stability Loss ◽

The Novel ◽

Step Potential ◽

Enzymatic Determination ◽

Wide Range ◽

Naoh Solution

CuxO-NiO nanocomposite film for the non-enzymatic determination of glucose was prepared by the novel modifying method. At first, anodized Cu electrode was kept in a mixture solution of CuSO4, NiSO4 and H2SO4 for 15 minutes. Then, a cathodization process with a step potential of -6 V in a mixture solution of CuSO4 and NiSO4 was initiated, generating formation of porous Cu-Ni film on the bare Cu electrode by electrodeposition assisted by the release of hydrogen bubbles acting as soft templates. Optimized conditions were determined by the experimental design software for electrodeposition process. Afterward, Cu-Ni modified electrode was scanned by cyclic voltammetry (CV) method in NaOH solution to convert Cu and Ni nanoparticles to the nano-scaled CuxO-NiO film. The electrocatalytic behavior of the novel CuxO-NiO film toward glucose oxidation was studied by CV and chronoamperometry (CHA) techniques. The calibration curve of glucose was found linear in a wide range of 0.04–5.76 mM, with a low limit of detection (LOD) of 7.3 µM (S/N = 3) and high sensitivity (1.38 mA mM-1 cm-2). The sensor showed high selectivity against some usual interfering species and high stability (loss of only 6.3 % of its performance over one month). The prepared CuxO-NiO nanofilm based sensor was successfully applied for monitoring glucose in human blood serum and urine samples.

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