scholarly journals A multipartite single-stranded negative-sense RNA virus is the putative agent of fig mosaic disease

2009 ◽  
Vol 90 (5) ◽  
pp. 1281-1288 ◽  
Author(s):  
Toufic Elbeaino ◽  
Michele Digiaro ◽  
Abdulkader Alabdullah ◽  
Angelo De Stradis ◽  
Angelantonio Minafra ◽  
...  
Keyword(s):  
Rna Virus ◽  
Amino Acid Level ◽  
Mosaic Disease ◽  
Glycoprotein Precursor ◽  
Mountain Ash ◽  
The Family ◽  
European Mountain ◽  
Novel Virus ◽  
Fig Mosaic Virus ◽  
Negative Sense

Several dsRNA bands (approx. 0.6–7 kbp in size) were recovered from tissues of mosaic-diseased fig seedlings which contained the enveloped round structures known as double membrane bodies (DMBs). blast analysis of a 4353 and a 1120 nt sequence from the two largest RNA segments showed homology with the polymerase and the putative glycoprotein precursor genes of negative-sense single-stranded RNA viruses of the family Bunyaviridae. Negative- and positive-sense riboprobes designed from both RNA segments hybridized to two bands of approximately 7 and 2.3 kbp in Northern blots of dsRNAs. Thus, these segments were identified as putative RNA-1 and RNA-2 of a novel virus for which the name fig mosaic virus (FMV) is proposed. Identity levels of predicted amino acids of the protein encoded by FMV RNA-1 with those of species of the family Bunyaviridae and European mountain ash ringspot-associated virus (EMERaV) were 28 and 54 %, respectively. RNA-2 showed 38 % identity at the amino acid level only with EMARaV. RNA-1 segment contained five conserved motifs (A–E) and an endonucleolytic centre of comparable genes of L RNA of bunyaviruses and EMARaV RNA-1. In a phylogenetic tree constructed with RdRp sequences, EMARaV grouped with FMV in a clade distinct from those of all bunyavirus genera. The consistent association of DMBs with mosaic symptoms and the results of molecular investigations strongly indicate that DMBs are particles of FMV, the aetiological agent of fig mosaic disease.

scholarly journals A novel, multipartite, negative-strand RNA virus is associated with the ringspot disease of European mountain ash (Sorbus aucuparia L.)

2007 ◽  
Vol 88 (4) ◽  
pp. 1337-1346 ◽  
Author(s):  
Nicole Mielke ◽  
Hans-Peter Muehlbach
Keyword(s):  
Rna Virus ◽  
Plant Viruses ◽  
Glycoprotein Precursor ◽  
Structure Comparison ◽  
Viral Rnas ◽  
Pathogenic Virus ◽  
Mountain Ash ◽  
The Family ◽  
European Mountain ◽  
Novel Virus

Four RNAs from a new plant-pathogenic virus, which we have tentatively named European mountain ash ringspot-associated virus (EMARAV), were identified and sequenced completely. All four viral RNAs could be detected in previous double-stranded RNA preparations. RNA 1 (7040 nt) encodes a protein with similarity to the RNA-dependent RNA polymerase of different members of the Bunyaviridae, a family containing five genera with viruses infecting invertebrates, vertebrates and plants. RNA 2 (2335 nt) encodes a 75 kDa protein containing a conserved motif of the glycoprotein precursor of the genus Phlebovirus. Immunological detection indicated the presence of proteins with the expected size of the precursor and one of its processing products. The amino acid sequence of protein p3 (35 kDa) encoded by RNA 3 shows similarities to a putative nucleocapsid protein of two still unclassified plant viruses. The fourth viral RNA encodes a 27 kDa protein that has no significant homology to any known protein. As is typical for members of the family Bunyaviridae, the 5′ and 3′ ends of all viral RNAs are complementary, which allows the RNA to form a panhandle structure. Comparison of these sequences demonstrates a conserved terminal part of 13 nt, similar to that of the bunyaviral genus Orthobunyavirus. Despite the high agreement of the EMARAV genome with several characteristics of the family Bunyaviridae, there are a few features that make it difficult to allocate the virus to this group. It is therefore more likely that this plant pathogen belongs to a novel virus genus.

scholarly journals Partial Sequence and Survey Analysis Identify a Multipartite, Negative-Sense RNA Virus Associated with Fig Mosaic

Plant Disease ◽  
2009 ◽  
Vol 93 (1) ◽  
pp. 4-10 ◽  
Author(s):  
Jeewan Jyot Walia ◽  
Nida M. Salem ◽  
Bryce W. Falk
Keyword(s):  
Nucleotide Sequence ◽  
Rna Virus ◽  
Germplasm Collection ◽  
Northern Hybridization ◽  
Survey Analysis ◽  
Mountain Ash ◽  
The Family ◽  
Specific Virus ◽  
European Mountain ◽  
Fig Trees

RNA and nucleotide sequence-based analyses were used to identify viruses in fig mosaic (FM)-affected fig (Ficus carica) trees. Nucleotide sequence analyses of 267 cloned cDNAs identified sequences corresponding to four viruses representing four distinct taxa from fig trees in California. Virus sequences corresponding to members of the family Closteroviridae were most common (55 sequences). We also found two sequences for an Umbravirus, one sequence corresponding to a Luteovirus-associated RNA, and two sequences that showed homology to European mountain ash ringspot-associated virus (EMARAV). Reverse transcription–polymerase chain reaction (RT-PCR) and northern hybridization analyses were used to confirm the presence of specific virus RNAs in fig trees. A survey of 184 fig trees from a germplasm collection, a commercial orchard, backyards, and feral fig trees showed that one virus was most common (detected in 96% of tested samples), while none of the other virus sequences were detected in more than 36% of the fig trees. Based on its association with FM-affected trees, nucleotide sequence-based phylogenetic association, and previous reported properties, we suggest the name of this virus as Fig mosaic-associated virus (FMaV).

scholarly journals Near-Complete Genome Sequence of a Novel Single-Stranded RNA Virus Discovered in Indoor Air

2018 ◽  
Vol 6 (12) ◽  
Author(s):  
Karyna Rosario ◽  
Noah Fierer ◽  
Mya Breitbart
Keyword(s):  
Genome Sequence ◽  
Indoor Air ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Air Conditioning ◽  
Rna Virus ◽  
Small Domain ◽  
Hvac Filters ◽  
Novel Virus ◽  
Negative Sense

ABSTRACT Viral metagenomic analysis of heating, ventilation, and air conditioning (HVAC) filters recovered the near-complete genome sequence of a novel virus, named HVAC-associated R NA v irus 1 (HVAC-RV1). The HVAC-RV1 genome is most similar to those of picorna-like viruses identified in arthropods but encodes a small domain observed only in negative-sense single-stranded RNA viruses.

scholarly journals Identification and characterization of two novel genomic RNA segments of fig mosaic virus, RNA5 and RNA6

2012 ◽  
Vol 93 (7) ◽  
pp. 1612-1619 ◽  
Author(s):  
Kazuya Ishikawa ◽  
Kensaku Maejima ◽  
Ken Komatsu ◽  
Yugo Kitazawa ◽  
Masayoshi Hashimoto ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Rna Virus ◽  
Mosaic Disease ◽  
The Novel ◽  
Genomic Rna ◽  
Evolutionary Patterns ◽  
Rna Molecules ◽  
Fig Mosaic Virus ◽  
Identification And Characterization

Fig mosaic virus (FMV), a negative-strand RNA virus, is recognized as a causal agent of fig mosaic disease. We performed RT-PCR for 14 FMV isolates collected from symptomatic fig plants in Japan and Serbia using primers corresponding to the conserved 13 nt stretches found at the termini of FMV genomic segments. The resulting simultaneous amplification of all FMV genomic segments yielded four previously identified segments of FMV and two novel segments. These novel FMV genomic RNA segments were found in each of the 14 FMV isolates analysed. In Northern blot studies, both the sense and antisense strands of these novel RNA molecules accumulated in FMV-infected fig leaves but not in uninfected fig leaves, confirming that they replicate as FMV genomic segments. Sequence analysis showed that the novel RNA segments are similar, in their structural organization and molecular evolutionary patterns, to those of known FMV genomic RNA segments. Our findings thus indicate that these newly discovered RNA segments are previously unidentified FMV genomic segments, which we have designated RNA5 and RNA6.

scholarly journals Vesicular Stomatitis Virus: From Agricultural Pathogen to Vaccine Vector

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1092
Author(s):  
Guodong Liu ◽  
Wenguang Cao ◽  
Abdjeleel Salawudeen ◽  
Wenjun Zhu ◽  
Karla Emeterio ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Reverse Genetics ◽  
Ebola Virus ◽  
Rna Virus ◽  
Research Tool ◽  
Vaccine Vector ◽  
The Family ◽  
Vesicular Stomatitis ◽  
Negative Sense ◽  
Family Rhabdoviridae

Vesicular stomatitis virus (VSV), which belongs to the Vesiculovirus genus of the family Rhabdoviridae, is a well studied livestock pathogen and prototypic non-segmented, negative-sense RNA virus. Although VSV is responsible for causing economically significant outbreaks of vesicular stomatitis in cattle, horses, and swine, the virus also represents a valuable research tool for molecular biologists and virologists. Indeed, the establishment of a reverse genetics system for the recovery of infectious VSV from cDNA transformed the utility of this virus and paved the way for its use as a vaccine vector. A highly effective VSV-based vaccine against Ebola virus recently received clinical approval, and many other VSV-based vaccines have been developed, particularly for high-consequence viruses. This review seeks to provide a holistic but concise overview of VSV, covering the virus’s ascension from perennial agricultural scourge to promising medical countermeasure, with a particular focus on vaccines.

scholarly journals ICTV Virus Taxonomy Profile: Mymonaviridae

2019 ◽  
Vol 100 (10) ◽  
pp. 1343-1344 ◽  
Author(s):  
Dàohóng Jiāng (姜道宏) ◽  
María A. Ayllón ◽  
Shin-Yi L. Marzano ◽  
Keyword(s):  
Filamentous Fungi ◽  
Sclerotinia Sclerotiorum ◽  
Single Molecule ◽  
Rna Virus ◽  
International Committee ◽  
Virus Taxonomy ◽  
Fungal Host ◽  
The Family ◽  
Single Genus ◽  
Negative Sense

Members of the family Mymonaviridae produce filamentous, enveloped virions containing a single molecule of linear, negative-sense RNA of ≈10 kb. The family currently includes a single genus, Sclerotimonavirus. Mymonaviruses usually infect filamentous fungi, and one virus, Sclerotinia sclerotiorum negative-stranded RNA virus 1, induces hypovirulence in the fungal host. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Mymonaviridae, which is available at ictv.global/report/mymonaviridae.

scholarly journals Coding-Complete Genome Sequence of a Human Respirovirus 1 Strain from a Clinical Sample in Arizona

2020 ◽  
Vol 9 (24) ◽  
Author(s):  
Matthew Scotch ◽  
Rolf U. Halden ◽  
Anthony Denton ◽  
Helen Sandrolini ◽  
Rafaela S. Fontenele ◽  
...  
Keyword(s):  
Complete Genome ◽  
Rna Virus ◽  
Clinical Sample ◽  
Health Clinic ◽  
Local Health ◽  
Swab Sample ◽  
Pharyngeal Swab ◽  
The Family ◽  
Negative Sense ◽  
Metagenomic Approach

ABSTRACT Human respirovirus 1 is a single-stranded, negative-sense RNA virus in the family Paramyxoviridae. Using a high-throughput metagenomic approach, we identified and sequenced the coding-complete genome of a human respirovirus 1 strain from a nasal pharyngeal swab sample from a local health clinic in Tempe, Arizona.

scholarly journals First Report of European mountain ash ringspot-associated virus in Sorbus aucuparia in Norway

Plant Disease ◽  
2014 ◽  
Vol 98 (5) ◽  
pp. 700-700 ◽  
Author(s):  
S. von Bargen ◽  
T. Büttner ◽  
H.-P. Mühlbach ◽  
J. Robel ◽  
C. Büttner
Keyword(s):  
Urban Forest ◽  
Forest Tree ◽  
Northern Europe ◽  
Native Plant ◽  
Sorbus Aucuparia ◽  
Glycoprotein Precursor ◽  
First Report ◽  
Mountain Ash ◽  
Ash Trees ◽  
European Mountain

In July 2012, leaf mottle and intensive chlorotic ringspots were observed on urban, forest, or roadside mountain ash trees (Sorbus aucuparia L., rowan) of different ages in Norway during visual inspection of native broadleaf forest tree species. Symptoms resembled those caused by European mountain ash ringspot-associated virus (EMARaV), the type-member of the newly established genus Emaravirus, containing segmented ss(-)RNA and infecting woody host species (2). Leaves of nine out of 30 assessed rowan trees exhibiting characteristic symptoms were sampled in the counties of Nordland and Nord-Trøndelag (between 63.511806° and 66.304680°N latitude). Three of them were infested by the potential vector the eriophyid gall mite Phytoptus pyri. EMARaV was detected from total RNA extracts of leaves by reverse transcription-PCR using virus-specific primers amplifying 300 bp of RNA2 and 204 bp of RNA3, respectively (3). PCR fragments were directly sequenced from both ends and submitted to the EMBL database (accession nos. HG428680 to 97). Sequenced fragments comprising the partial gene encoding the glycoprotein-precursor (261 nucleotides of RNA2 omitting primer sequences) obtained from the nine sampled trees showed identities of 97 to 98% to the sequence of the reference strain of EMARaV from Hamburg, Germany (database accession AY563041). Comparison of 159 nucleotides of the 3′ untranslated region (3′ UTR) of viral RNA3 of the nine investigated rowans in Norway exhibited higher sequence diversity on nucleotide level (up to 50 nucleotide exchanges, or 31%) as previously reported from EMARaV variants from other European countries (4). When subjected to BLASTn search through GenBank, only three partial RNA3 sequences generated in this study showed sequence identities of 96% to the reference isolate (accession DQ831831). The other six sequences revealed only 68 to 73% identity to RNA3 sequences of EMARaV variants from GenBank. This led to formation of a separate cluster in phylogenetic analysis of partial RNA3 sequences of the six EMARaV variants from Norway when compared to previously characterized strains from the Czech Republic (n = 2), Finland (n = 17), Germany (n = 1), Great Britain (n = 5), Russia (n = 3), and Sweden (n = 10). From three Norwegian samples clustering separately in the tree based on the partial 3′ UTR of RNA3, the partial vRNA1 was amplified by RT-PCR using a generic primer set Motif-A-sense/Motif-C-antisense (1). Sequence analyses of these PCR fragments confirmed the viruses as members of the Emaravirus genus which were most closely related to EMARaV (data not shown). This is the first report of EMARaV in Norway infecting Sorbus aucuparia, a valuable native plant of northern Europe. The data obtained suggest a higher genetic variability of the EMARaV population in mountain ash trees in Norway than in other locations in Central and Northern Europe. However, whether the EMARaV variants identified in this study represent new strains of the virus have to be investigated in the future. References: (1) T. Elbeaino et al. J. Virol. Meth. 188:37, 2013. (2) N. Mielke-Ehret. and H. P. Mühlbach. Viruses 4:1515, 2012. (3) N. Mielke et al. For. Pathol. 38:371, 2008. (4) S. von Bargen et al. For. Pathol. 43: 429, 2013.

Enterovirus D-68 Molecular Virology, Epidemiology, and Treatment: an update and way forward

2020 ◽  
Vol 20 ◽  
Author(s):  
Mahmoud Ahmed Ebada ◽  
Notila Fayed ◽  
Souad Alkanj ◽  
Ahmed Wadaa Allah
Keyword(s):  
Current Knowledge ◽  
Rna Virus ◽  
Neurological Diseases ◽  
Cross Reactivity ◽  
Serological Tests ◽  
Molecular Virology ◽  
Acute Flaccid Myelitis ◽  
The Family ◽  
Pcr Products ◽  
Enterovirus D68

: Enterovirus D68 (EV-D68) is a single-stranded positive-sense RNA virus, and it is one of the family Picornaviridae. Except for EV-D68, the family Picornaviridae has been illustrated in literature. EV-D68 was first discovered and isolated in California, USA, in 1962. EV-D68 has resulted in respiratory disorders’ outbreaks among children worldwide, and it has been detected in cases of various neurological diseases such as acute flaccid myelitis (AFM). A recent study documented a higher number of EV-D68 cases associated with AFM in Europe in 2016 compared to the 2014 outbreak. EV-D68 is mainly diagnosed by quantitative PCR, and there is an affirmative strategy for EV-D68 detection by using pan-EV PCR on the untranslated region and/or the VP1 or VP2, followed by sequencing of the PCR products. Serological tests are limited due to cross-reactivity of the antigens between the different serotypes. Many antiviral drugs for EV-D68 have been evaluated, and showed promising results. In our review, we discuss the current knowledge about EV-D68 and its role in the development of AFM.

scholarly journals Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement

2013 ◽  
Vol 94 (3) ◽  
pp. 682-686 ◽  
Author(s):  
Kazuya Ishikawa ◽  
Kensaku Maejima ◽  
Ken Komatsu ◽  
Osamu Netsu ◽  
Takuya Keima ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Laser Scanning ◽  
Movement Protein ◽  
Rna Virus ◽  
Cell Movement ◽  
Plant Cells ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Series Of Experiments ◽  
Fig Mosaic Virus

Fig mosaic virus (FMV), a member of the newly formed genus Emaravirus, is a segmented negative-strand RNA virus. Each of the six genomic FMV segments contains a single ORF: that of RNA4 encodes the protein p4. FMV-p4 is presumed to be the movement protein (MP) of the virus; however, direct experimental evidence for this is lacking. We assessed the intercellular distribution of FMV-p4 in plant cells by confocal laser scanning microscopy and we found that FMV-p4 was localized to plasmodesmata and to the plasma membrane accompanied by tubule-like structures. A series of experiments designed to examine the movement functions revealed that FMV-p4 has the capacity to complement viral cell-to-cell movement, prompt GFP diffusion between cells, and spread by itself to neighbouring cells. Altogether, our findings demonstrated that FMV-p4 shares several properties with other viral MPs and plays an important role in cell-to-cell movement.

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