scholarly journals Rewiring of gene expression in circulating white blood cells is associated with pregnancy outcome in heifers (Bos taurus)

2020 ◽  
Author(s):  
Sarah E. Moorey ◽  
Bailey N. Walker ◽  
Michelle F. Elmore ◽  
Joshua B. Elmore ◽  
Soren P. Rodning ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pregnancy Outcome ◽  
Artificial Insemination ◽  
Blood Cells ◽  
Expression Profiles ◽  
White Blood Cells ◽  
Transcript Abundance ◽  
Prediction Algorithm ◽  
Differential Coexpression ◽  
Micro Rnas

ABSTRACTInfertility is a challenging phenomenon in cattle that reduces the sustainability of beef production worldwide. Here, we tested the hypothesis that gene expression profiles of protein-coding genes expressed in peripheral white blood cells (PWBCs), and circulating micro RNAs in plasma, are associated with female fertility, measured by pregnancy outcome. We drew blood samples from 17 heifers on the day of artificial insemination and analyzed transcript abundance for 10496 genes in PWBCs and 290 circulating micro RNAs. The females were later classified as pregnant to artificial insemination, pregnant to natural breeding or not pregnant. We identified 1860 genes producing significant differential coexpression (eFDR<0.002) based on pregnancy outcome. Additionally, 237 micro RNAs and 2274 genes in PWBCs presented differential coexpression based on pregnancy outcome. Furthermore, using a machine learning prediction algorithm we detected a subset of genes whose abundance could be used for blind categorization of pregnancy outcome. Our results provide strong evidence that transcript abundance in circulating white blood cells is associated with fertility in heifers.

scholarly journals Rewiring of gene expression in circulating white blood cells is associated with pregnancy outcome in heifers (Bos taurus)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sarah E. Moorey ◽  
Bailey N. Walker ◽  
Michelle F. Elmore ◽  
Joshua B. Elmore ◽  
Soren P. Rodning ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pregnancy Outcome ◽  
Artificial Insemination ◽  
Blood Cells ◽  
Expression Profiles ◽  
White Blood Cells ◽  
Transcript Abundance ◽  
Prediction Algorithm ◽  
Differential Coexpression ◽  
Micro Rnas

Abstract Infertility is a challenging phenomenon in cattle that reduces the sustainability of beef production worldwide. Here, we tested the hypothesis that gene expression profiles of protein-coding genes expressed in peripheral white blood cells (PWBCs), and circulating micro RNAs in plasma, are associated with female fertility, measured by pregnancy outcome. We drew blood samples from 17 heifers on the day of artificial insemination and analyzed transcript abundance for 10,496 genes in PWBCs and 290 circulating micro RNAs. The females were later classified as pregnant to artificial insemination, pregnant to natural breeding or not pregnant. We identified 1860 genes producing significant differential coexpression (eFDR < 0.002) based on pregnancy outcome. Additionally, 237 micro RNAs and 2274 genes in PWBCs presented differential coexpression based on pregnancy outcome. Furthermore, using a machine learning prediction algorithm we detected a subset of genes whose abundance could be used for blind categorization of pregnancy outcome. Our results provide strong evidence that transcript abundance in circulating white blood cells is associated with fertility in heifers.

Effects Of Acute Exercise On Gene Expression Profiles In White Blood Cells

2005 ◽  
Vol 37 (Supplement) ◽  
pp. S336
Author(s):  
Frank C. Mooren ◽  
Petra Buettner ◽  
Sandy Mosig ◽  
Anja Lechtermann ◽  
Klaus Völker ◽  
...  
Keyword(s):  
Gene Expression ◽  
Blood Cells ◽  
Acute Exercise ◽  
Expression Profiles ◽  
White Blood Cells ◽  
Gene Expression Profiles

Effects Of Acute Exercise On Gene Expression Profiles In White Blood Cells

2005 ◽  
Vol 37 (Supplement) ◽  
pp. S336
Author(s):  
Frank C. Mooren ◽  
Petra Buettner ◽  
Sandy Mosig ◽  
Anja Lechtermann ◽  
Klaus V??lker ◽  
...  
Keyword(s):  
Gene Expression ◽  
Blood Cells ◽  
Acute Exercise ◽  
Expression Profiles ◽  
White Blood Cells ◽  
Gene Expression Profiles

196 EMBRYO BIOPSY TRANSCRIPTOMICS: A POTENTIAL TOOL TO IDENTIFY TRANSCRIPTS DIRECTLY RELATED TO THE ABILITY OF THE EMBRYO TO INDUCE PREGNANCY AFTER TRANSFER

2009 ◽  
Vol 21 (1) ◽  
pp. 196
Author(s):  
D. Tesfaye ◽  
N. Ghanem ◽  
F. Rings ◽  
E. Tholen ◽  
C. Phatsara ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pregnancy Outcome ◽  
Expression Profile ◽  
Expression Profiles ◽  
Transcript Abundance ◽  
Differentially Expressed ◽  
Embryo Biopsy ◽  
Bovine Embryo

The incidence of pregnancy loss due to embryonic mortality in cattle is one of the major causes of reproductive failure. The early embryonic loss can be due to problems with the embryo itself, the uterine environment, or interactions between the embryo and the uterus. So, this study was conducted to investigate the gene expression profile of bovine embryo biopsies produced in vivo and in vitro that resulted in different pregnancy outcomes. For this, biopsies representing 30 to 40% of the intact in vitro and in vivo blastocysts were taken, and 60 to 70% part was allowed to re-expand prior to transfer to recipients. Based on the pregnancy outcome after transfer, biopsies (n = 10 per pool) were grouped into 3 distinct phenotypes: those that resulted in no pregnancy, those that resulted in resorption, and those that resulted in successful pregnancy and subsequent calf delivery. A bovine cDNA microarray with 2000 clones was used to analyze the gene expression profiles of 3 replicates from each embryo biopsy group. Array data analysis revealed a total of 50 and 52 genes to be differentially expressed between biopsies derived from in vivo blastocysts that resulted in no pregnancy v. calf delivery and resorption v. calf delivery, respectively. Similarly, a total of 52 and 58 transcripts were differentially expressed between biopsies derived from in vitro-produced blastocysts that resulted in no pregnancy v. calf delivery and resorption v. calf delivery, respectively. Quantitative real-time PCR has confirmed the expression profile of 6 selected candidate genes. A distinct set of genes were found to be commonly expressed between in vitro- and in vivo-derived blastocyst biopsies, which ended up with the same pregnancy outcome. Biopsies, which ended up with calf delivery, were found to be enriched with transcripts involved in nucleosome assembly (KRT8), translation (RPLPO), electron transport (COX-2), and placenta specific (PLAC8). On the other hand, transcripts regulating immune response (TNFa), response to stress (HSPD1), and cell adhesion (CD9) were up-regulated in embryos that resulted in no pregnancy or resorption. Differences in transcript abundance of some genes have been seen between biopsies derived from in vitro and in vivo blastocysts. Biopsies from in vivo-derived blastocysts and that ended up with resorption were found to be enriched with transcripts regulating calcium-binding protein (S100A10, S100A14). Transcription factor-related transcripts (CDX2, HOXB7) were up-regulated in vitro-derived blastocyst biopsies that resulted in no pregnancy. In conclusion, the results evidenced that embryos derived from either in vitro or in vivo have more similarities than differences in their transcript abundance with respect to the ability in initiating pregnancy.

Exercise affects the gene expression profiles of human white blood cells

2007 ◽  
Vol 102 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Petra Büttner ◽  
Sandy Mosig ◽  
Anja Lechtermann ◽  
Harald Funke ◽  
Frank C. Mooren
Keyword(s):  
Gene Expression ◽  
Blood Cells ◽  
Expression Profiles ◽  
White Blood Cells ◽  
Gene Expression Profiles ◽  
Response Analysis ◽  
Treadmill Test ◽  
Expression Levels ◽  
Exercise And Training ◽  
And Training

White blood cells (WBCs) express tens of thousands of genes, whose expression levels are modified by genetic and external factors. The purpose of the present study was to investigate the effects of acute exercise on gene expression profiles (GEPs) of WBCs and to identify suitable genes that may serve as surrogate markers for monitoring exercise and training load. Five male participants performed an exhaustive treadmill test (ET) at 80% of their maximal O2uptake (V̇o2 max) and a moderate treadmill test (MT) at 60% V̇o2 maxfor exactly the same time ∼2 wk later. WBCs were isolated by the erythrocyte lysis method. GEPs were measured using the Affymetrix GeneChip technology. After scaling, normalization, and filtering, groupwise comparisons of gene expression intensities were performed, and several measurements were validated by real-time PCR. We found 450 genes upregulated and 150 downregulated (>1.5-fold change; ANOVA with Benjamini-Hochberg correction, P < 0.05) after ET that were closely associated with the gene ontology lists “response to stress” and “inflammatory response”. Analysis of mean expression levels after MT showed that the extent of up- and downregulation was workload dependent. The genes for the stress (heat shock) proteins HSPA1A and HSPH1 and for the matrix metalloproteinase MMP-9 showed the most prominent increases, whereas the YES1 oncogene (YES1) and CD160 (BY55) were most strongly reduced. Despite different methodological approaches used, the consistency of our results with the expression data of another study (Connolly PH, Caiozzo VJ, Zaldivar F, Nemet D, Larson J, Hung SP, Heck JD, Hatfield GW, Cooper DM. J Appl Physiol 97: 1461–1469, 2004) suggests that expression fingerprints are useful tools for monitoring exercise and training loads and thereby help to avoid training-associated health risks.

Gene Expression Profiles in White Blood Cells of Volunteers Exposed to a 50 Hz Electromagnetic Field

2012 ◽  
Vol 178 (3) ◽  
pp. 138 ◽  
Author(s):  
Heide Kirschenlohr ◽  
Peter Ellis ◽  
Robin Hesketh ◽  
James Metcalfe
Keyword(s):  
Gene Expression ◽  
Electromagnetic Field ◽  
Blood Cells ◽  
Expression Profiles ◽  
White Blood Cells ◽  
Gene Expression Profiles

Gene Expression Differences in White Blood Cells after Escherichia coli Infection in Chickens

2012 ◽  
Author(s):  
Erin Sandford ◽  
Megan Orr ◽  
Xianyao Li ◽  
Huaijun Zhou ◽  
timothy J. Johnson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Blood Cells ◽  
White Blood Cells ◽  
Escherichia Coli Infection

scholarly journals Gene expression of the heat stress response in bovine peripheral white blood cells and milk somatic cells in vivo

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
J. B. Garner ◽  
A. J. Chamberlain ◽  
C. Vander Jagt ◽  
T. T. T. Nguyen ◽  
B. A. Mason ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Blood Cells ◽  
White Blood Cells ◽  
Somatic Cells ◽  
Holstein Friesian ◽  
Milk Somatic Cells ◽  
Peripheral White Blood Cells ◽  
Friesian Cows

Abstract Heat stress in dairy cattle leads to reduction in feed intake and milk production as well as the induction of many physiological stress responses. The genes implicated in the response to heat stress in vivo are not well characterised. With the aim of identifying such genes, an experiment was conducted to perform differential gene expression in peripheral white blood cells and milk somatic cells in vivo in 6 Holstein Friesian cows in thermoneutral conditions and in 6 Holstein Friesian cows exposed to a short-term moderate heat challenge. RNA sequences from peripheral white blood cells and milk somatic cells were used to quantify full transcriptome gene expression. Genes commonly differentially expressed (DE) in both the peripheral white blood cells and in milk somatic cells were associated with the cellular stress response, apoptosis, oxidative stress and glucose metabolism. Genes DE in peripheral white blood cells of cows exposed to the heat challenge compared to the thermoneutral control were related to inflammation, lipid metabolism, carbohydrate metabolism and the cardiovascular system. Genes DE in milk somatic cells compared to the thermoneutral control were involved in the response to stress, thermoregulation and vasodilation. These findings provide new insights into the cellular adaptations induced during the response to short term moderate heat stress in dairy cattle and identify potential candidate genes (BDKRB1 and SNORA19) for future research.

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