scholarly journals Quantitative mapping of membrane nanoenvironments through single-molecule imaging of solvatochromic probes

2020 ◽  
Author(s):  
Daniel J. Nieves ◽  
Dylan M. Owen
Keyword(s):  
Plasma Membrane ◽  
Quantitative Analysis ◽  
Cell Membrane ◽  
Single Molecule ◽  
Marked Point ◽  
Membrane Properties ◽  
Point Patterns ◽  
Quantitative Mapping ◽  
Solvatochromic Probes ◽  
Environmentally Sensitive

AbstractEnvironmentally-sensitive fluorophores report on their local biochemical or biophysical environments through changes in their emission. We combine the solvatochromic probe di-4-ANEPPDHQ with multi-channel SMLM and quantitative analysis of the resulting marked point patterns to map biophysical environments in the mammalian cell membrane. We show that plasma membrane properties can be mapped with nanoscale resolution, and that partitioning between ordered and disordered regions is observed on length scales below 300 nm.

Study on erythrocytes by SEM photogrammetry (SEMP) technique

1990 ◽  
Vol 48 (3) ◽  
pp. 724-725
Author(s):  
Tong Wensheng ◽  
Lu Lianhuang ◽  
Zhang Zhijun
Keyword(s):  
Quantitative Analysis ◽  
Cell Membrane ◽  
Clinical Diagnosis ◽  
Human Body ◽  
Blood Cells ◽  
Three Dimensional ◽  
Normal Person ◽  
Blood Disease ◽  
Computation Technique ◽  
Important Basis

This is a combined study of two diffirent branches, photogrammetry and morphology of blood cells. The three dimensional quantitative analysis of erythrocytes using SEMP technique, electron computation technique and photogrammetry theory has made it possible to push the study of mophology of blood cells from LM, TEM, SEM to a higher stage, that of SEM P. A new path has been broken for deeply study of morphology of blood cells.In medical view, the abnormality of the quality and quantity of erythrocytes is one of the important changes of blood disease. It shows the abnormal blood—making function of the human body. Therefore, the study of the change of shape on erythrocytes is the indispensable and important basis of reference in the clinical diagnosis and research of blood disease.The erythrocytes of one normal person, three PNH Patients and one AA patient were used in this experiment. This research determines the following items: Height;Length of two axes (long and short), ratio; Crevice in depth and width of cell membrane; Circumference of erythrocytes; Isoline map of erythrocytes; Section map of erythrocytes.

scholarly journals Quantitative Analysis of the Membrane Affinity of Local Anesthetics Using a Model Cell Membrane

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 579
Author(s):  
Wanjae Choi ◽  
Hyunil Ryu ◽  
Ahmed Fuwad ◽  
Seulmini Goh ◽  
Chaoge Zhou ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Local Anesthetics ◽  
Electrophysiological Study ◽  
Local Area ◽  
Membrane Properties ◽  
Dominant Factor ◽  
Free Standing ◽  
Membrane Affinity ◽  
Conformational Effects ◽  
Model Protein

Local anesthesia is a drug that penetrates the nerve cell membrane and binds to the voltage gate sodium channel, inhibiting the membrane potential and neurotransmission. It is mainly used in clinical uses to address the pain of surgical procedures in the local area. Local anesthetics (LAs), however, can be incorporated into the membrane, reducing the thermal stability of the membrane as well as altering membrane properties such as fluidity, permeability, and lipid packing order. The effects of LAs on the membrane are not yet fully understood, despite a number of previous studies. In particular, it is necessary to analyze which is the more dominant factor, the membrane affinity or the structural perturbation of the membrane. To analyze the effects of LAs on the cell membrane and compare the results with those from model membranes, morphological analysis and 50% inhibitory concentration (IC50) measurement of CCD-1064sk (fibroblast, human skin) membranes were carried out for lidocaine (LDC) and tetracaine (TTC), the most popular LAs in clinical use. Furthermore, the membrane affinity of the LAs was quantitatively analyzed using a colorimetric polydiacetylene assay, where the color shift represents their distribution in the membrane. Further, to confirm the membrane affinity and structural effects of the membranes, we performed an electrophysiological study using a model protein (gramicidin A, gA) and measured the channel lifetime of the model protein on the free-standing lipid bilayer according to the concentration of each LA. Our results show that when LAs interact with cell membranes, membrane affinity is a more dominant factor than steric or conformational effects of the membrane.

scholarly journals Phenothiazines alter plasma membrane properties and sensitize cancer cells to injury by inhibiting annexin-mediated repair

2021 ◽  
pp. 101012
Author(s):  
Anne Sofie Busk Heitmann ◽  
Ali Asghar Hakami Zanjani ◽  
Martin Berg Klenow ◽  
Anna Mularski ◽  
Stine Lauritzen Sønder ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Cancer Cells ◽  
Membrane Properties

Modeling the Mechanics of Tethers Pulled From the Cochlear Outer Hair Cell Membrane

2008 ◽  
Vol 130 (3) ◽  
Author(s):  
Kristopher R. Schumacher ◽  
Aleksander S. Popel ◽  
Bahman Anvari ◽  
William E. Brownell ◽  
Alexander A. Spector
Keyword(s):  
Cell Membrane ◽  
Hair Cell ◽  
Outer Hair Cell ◽  
Lateral Wall ◽  
Outer Hair Cells ◽  
The Body ◽  
Computational Method ◽  
Membrane Properties ◽  
Bending Modulus ◽  
Sound Amplification

Cell membrane tethers are formed naturally (e.g., in leukocyte rolling) and experimentally to probe membrane properties. In cochlear outer hair cells, the plasma membrane is part of the trilayer lateral wall, where the membrane is attached to the cytoskeleton by a system of radial pillars. The mechanics of these cells is important to the sound amplification and frequency selectivity of the ear. We present a modeling study to simulate the membrane deflection, bending, and interaction with the cytoskeleton in the outer hair cell tether pulling experiment. In our analysis, three regions of the membrane are considered: the body of a cylindrical tether, the area where the membrane is attached and interacts with the cytoskeleton, and the transition region between the two. By using a computational method, we found the shape of the membrane in all three regions over a range of tether lengths and forces observed in experiments. We also analyze the effects of biophysical properties of the membrane, including the bending modulus and the forces of the membrane adhesion to the cytoskeleton. The model’s results provide a better understanding of the mechanics of tethers pulled from cell membranes.

Four-Color Single-Molecule Imaging with Engineered Tags Resolves the Molecular Architecture of Signaling Complexes in the Plasma Membrane

2021 ◽  
Author(s):  
Junel Sotolongo Bellón ◽  
Oliver Birkholz ◽  
Christian Paolo Richter ◽  
Florian Eull ◽  
Hella Kenneweg ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Single Molecule ◽  
Single Molecule Imaging ◽  
Molecular Architecture
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