scholarly journals Characterization, and Anti-microbial Properties of Zinc Oxide Nanoparticles Synthesized Using Citrus sinensis (L.) Osbeck Peel Extract

2020 ◽  
Author(s):  
Dharma Prasad Khanal ◽  
Sadikshya Aryal ◽  
Samyam Aryal
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Citrus Sinensis ◽  
Zinc Oxide Nanoparticles ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Oxide Nanoparticles ◽  
Ms Analysis ◽  
Aqueous Solvent ◽  
Peel Extract

ABSTRACTBackgroundCitrus sinensis (L.) Osbeck peels are usually discarded as wastes; however, they are rich sources of Vitamin C, fibre, and many nutrients including phenolics and flavonoids which are also good antioxidant agents. This study aimed to examine phytochemical composition, antioxidant capabilities, cytotoxicity of C. sinensis (L.) Osbeck peel extract and and to compare the antibacterial activity with zinc nanoparticles of Citrus sinensis (L.) Osbeck peels with its extract. GC-MS analysis of the compounds present in the peels extract of Citrus sinensis (L.) Osbeck was also done.MethodsC. sinensis (L.) Osbeck fruits were collected from Sindhuli district and were taken to National Herbarium and Plant Laboratory, Godawari, Lalitpur for its identification. Extraction was done by maceration in aqueous solvent. Extract was subjected to Phytochemical screening done by color reactions with different reagents, Antioxidant activities of the peel extracts were examined via the 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity. Total phenolic content and total flavonoid content of the extracts were measured via the Folin-Ciocalteau method and the aluminium chloride colorimetric method, respectively. Cytotoxic activities of the peel extracts were determined by Brine Shrimp Lethality Bioassay. Comparison of antibacterial activity of extract and zinc oxide nanoparticles prepared via green synthesis using C. sinensis (L.) Osbeck peel extracts as reducing agents. Antibacterial activity was tested by Bore well diffusion method.ResultThe extractive value of C. sinensis (L.) Osbeck was found to be 8.64% in aqueous solvent. GC-MS analysis of peel extract of C. sinensis (L.) Osbeck showed the presence of 2-Methoxy-4-vinylphenol, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl, Benzoic acid, 3-Deoxy-d-mannoic lactone and 5-Hydroxymethyl-furfuralas major compounds. The qualitative phytochemical test showed the presence of tannin, alkaloid, carbohydrate, flavonoid, cardiac glycoside, terpinoid.The DPPH radical scavenging activity of C. sinensis (L.) Osbeck peel extract was 35.56 μg/ml. TPC of C. sinensis (L.) Osbeck peel extracts was 46.07 mg GAE/g. TFC was 1.29 mg QE/g. The LD50 value of Brine Shrimp Lethality assay of the extract showed 312.5μg/ml which is indicative. The antibacterial activity of zinc oxide nanoparticles was found to be greater than that of the extract, but the antibacterial activity of Zn-NPs was less than that of the standard. ConclusionHence, the GC-MS analysis of aqueous extracts of leaves of C. sinensis (L.) Osbeck showed the presence of 20 different compounds. Phytochemicals including phenolics and flavonoids in C. sinensis (L.) Osbeck peel extracts exhibited good antioxidant properties. The extract also exhibited antibacterial activity which was 4 times less than that of the standard. The antibacterial activity of standard was 2 times greater than that of Zn-NPs. The extract also exhibited cytotoxic activity. This study indicated that C. sinensis (L.) Osbeck peels contained potential antioxidant, cytotoxic and antibacterial compounds which could be exploited as value added products.

scholarly journals Free Radical Scavenging Activity of Fruit Formulation Mediated Zinc Oxide Nanoparticles

2020 ◽  
Vol 11 (3) ◽  
pp. 4690-4694
Author(s):  
Tahoora Taskeen. L ◽  
Hannah R ◽  
Rajeshkumar S
Keyword(s):  
Antioxidant Activity ◽  
Zinc Oxide ◽  
Free Radicals ◽  
Zinc Oxide Nanoparticles ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Punica Granatum ◽  
Free Radical Scavenging ◽  
Oxide Nanoparticles ◽  
Scavenging Activity

To determine the free radical scavenging activity of fruit formulation mediated zinc oxide nanoparticle. Zinc oxide nanoparticles are widely known for their antimicrobial agent, anti-diabetic, anti-inflammatory, wound healing, antioxidant and optic properties. Oxidative degradation of lipids, proteins, food and cosmetics involves a radical-chain reaction with release of free radicals. These free radicals are extremely reactive and can even attack relatively stable molecules like the DNA bases. With a recent rise in diseases associated with increased oxidative stress, a good antioxidant seems to be the need of the hour. Synthesis of zinc oxide nanoparticles using Punica granatum and Elettaria cardamomum extract. Characterisation of the nanoparticles using UV–Visible spectroscopy and Scanning electron microscope. It is followed by the DPPH radical scavenging assay to determine the antioxidant activity. With an increase in the concentration of zinc oxide nanoparticles, there was an increase in the percentage of inhibition. When compared to the standard, the zinc oxide nanoparticles showed increased antioxidant activity even in small concentrations. Punica granatum and Elettaria cardamomum mediated Zinc oxide nanoparticles showed considerable antioxidant property even in small quantity when compared with standard vitamin C. Further studies need to be carried out to check for its potency as a prophylactic agent.

Sunflower Assisted Bio-derived ZnO-NPs as an Efficient Nanocatalyst for Synthesis of Novel Quinazolines with Highly Antioxidant Activities

2021 ◽  
Author(s):  
Balaji GL ◽  
S. Mahesh ◽  
Boya Palajonnala Narasaiah ◽  
Sadegh Rostamnia ◽  
Harihara Padhy ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Antioxidant Activities ◽  
Present Report ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Green Method ◽  
Vis Spectroscopy

Abstract The present report an environmental benign route for the fabrication of Zinc Oxide nanoparticles (ZnO-NPs) using sunflower leaf aqueous extract at room temperature. This was an green method is a rapid biogenic and offers few advantages over the chemical and physical procedures, as it is an easy and fast, eco-friendly and does not involve any costly chemicals as well as hazardous chemicals, harmful solvent. The synthesized ZnO-NPs were characterized using different technique such as UV-Visible spectroscopy. The formation of ZnO-NPs was confirmed by Surface Plasmon Resonance (SRP) at 344 nm using UV-Vis spectroscopy. The leaf extract act as a source of phyto-chemicals and are the primarily responsible for the reduction and then formation of stable Zinc Oxide nanoparticles by the characteristic functional groups of extract and synthesized ZnO-NPs were identified by FTIR spectroscopy. Crystalline nature of ZnO-NPs was confirmed by powder –XRD. Size and morphology were measured by HR-TEM analysis. Stability of the nanoparticles is represented by DLS and TGA analysis. The synthesized ZnO-NPs has been found good and efficient catalyst for the synthesis of novel 1,2-dihydroquinazoline derivatives under green method via one pot reaction of 2-amino benzophenone, 1,3-diphenyl-1 H -pyrazole carbaldehydes and ammonium acetate. The synthesized compounds (4a-o) were characterized by 1 HNMR, 13 CNMR and HRMS spectra and further screened for radical scavenging activity. Among all the compounds showed good percentage of inhibition against standard ascorbic acid. The synthesized ZnO nanoparticles showed good antioxidant activity and also act as a good promising material for catalytic agent.

Antibacterial Activity of Zinc Oxide Nanoparticles Using Banana Peel Extract against Antibiotic-Resistant Bacteria

2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Andrew Melencion ◽  
◽  
Winson Gutierrez ◽  
Merced Melencion ◽  
◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Musa Acuminata ◽  
Banana Peel ◽  
Resistant Bacteria ◽  
Oxide Nanoparticles ◽  
Antibiotic Resistant ◽  
Antimicrobial Nanoparticles ◽  
Peel Extract

antimicrobial, nanoparticles, Musa acuminata × balbisiana

A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Hams H. H. Alfattli ◽  
Ghufran Zuhair Jiber ◽  
Ghaidaa Gatea Abbass
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Quercus Robur ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Oxide Nanoparticles ◽  
Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

Evaluation of antioxidant enzyme content, phenolic content, and antibacterial activity of Commiphora gileadensis grown in Saudi Arabia

2021 ◽  
Vol 19 (4) ◽  
pp. 329-343
Author(s):  
Yaaser Q. Almulaiky ◽  
Ammar AL-Farga
Keyword(s):  
Antibacterial Activity ◽  
Saudi Arabia ◽  
Oral Hygiene ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Reference Drug ◽  
Wide Range ◽  
Peel Extract

Commiphora gileadensis is commonly used in Saudi Arabia for oral hygiene. A lack of data about its biological activity encouraged us to evaluate the antioxidant and antibacterial activities of its leaf and stem extracts. Ethanol, methanol, acetone and deionized water were tested as extraction solvents. 80% methanol gave the highest extracted concentrations of phenolic and flavonoid substances. The leaf and stem extracts were respectively evaluated for their radical scavenging activity with DPPH (EC50 = 3.39, and 1.06), ABTS (EC50 = 0.690, and 0.55), and peroxide scavenging activity (EC50 = 2.43, and 1.28). GC-MS identified a wide range of compounds that may be responsible for these activities of the results observed. The highest levels of chlorophyll, carotenoids, and lycopene were found in the leaf extract while level of proanthocyanidins was found in the stem peels extract. The peroxidase and catalase activities of stem peel extract were higher than those of the leaf extract. The findings showed that the leaf and stem peel extracts of C. gileadensis exhibited significant antibacterial activity against the test organisms. The minimum inhibitory concentrations for the plant extracts were compared with the standard reference drug Augmentin but the time–kill curves for the C. gileadensis extracts showed that they were less effective than Augmentin. Moreover, the stem peel extract exhibited stronger antibacterial activity than the leaf extract. In conclusion, C. gileadensis can be an important source of natural antioxidants, used as a healthy chewing stick for teeth brushing and oral hygiene purposes.

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