scholarly journals A novel family of nonribosomal peptides modulate collective behavior in Pseudovibrio bacteria isolated from marine sponges

2020 ◽  
Author(s):  
Laura P Ioca ◽  
Yitao Dai ◽  
Sylvia Kunakom ◽  
Jennifer Diaz-Espinosa ◽  
Aleksej Krunic ◽  
...  
Keyword(s):  
Collective Behavior ◽  
Reverse Genetics ◽  
Polyketide Synthase ◽  
Current Knowledge ◽  
Imaging Mass Spectrometry ◽  
Marine Sponges ◽  
Bacterial Survival ◽  
Swarming Motility ◽  
Collective Behaviors ◽  
Polyketide Synthase Gene

Collective behavior is a common feature of life. Although swarming motility and biofilms are opposed collective behaviors, both contribute to bacterial survival and host colonization. We have identified a link between motility/biofilms and a nonribosomal peptide synthetase-polyketide synthase gene cluster family (ppp) conserved in Pseudovibrio and Pseudomonas Proteobacteria known to interact with diverse eukaryotes. After developing reverse genetics for Pseudovibrio, we discovered two pseudovibriamide families, heptapeptides with a reversal in chain polarity via an ureido linkage 1-6 and related nonadepsipeptides 7-12. Imaging mass spectrometry showed that 1 was excreted whereas 7 was colony-associated. Deletion of pppA abolished production of 1-12 leading to reduced motility and increased biofilm production. pppD mutants that produced only 1-6 showed motility comparable to the wild-type and reduced biofilm formation, indicating that the excreted heptapeptides play a role in promoting motility. In contrast to lipopeptides widely known to affect swarming and biofilms, pseudovibriamides are not surfactants. Our results expand current knowledge on metabolites mediating bacterial collective behavior. Moreover, the establishment of reverse genetics will enable future exploration of the ecological and biotechnological potential of Pseudovibrio bacteria which have been proposed to contribute to marine sponge health.

scholarly journals Polyketide Synthase Gene Diversity within the Microbiome of the Sponge Arenosclera brasiliensis, Endemic to the Southern Atlantic Ocean

2012 ◽  
Vol 79 (5) ◽  
pp. 1598-1605 ◽  
Author(s):  
Amaro E. Trindade-Silva ◽  
Cintia P. J. Rua ◽  
Bruno G. N. Andrade ◽  
Ana Carolina Paulo Vicente ◽  
Genivaldo G. Z. Silva ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Polyketide Synthase ◽  
Gene Diversity ◽  
Marine Sponges ◽  
Type I ◽  
Content Type ◽  
Phylogenetic Reconstructions ◽  
Polyketide Synthase Gene ◽  
Exclusive Group ◽  
Southern Atlantic Ocean

ABSTRACTMicrobes associated with marine sponges are considered important producers of bioactive, structurally unique polyketides. The synthesis of such secondary metabolites involves type I polyketide synthases (PKSs), which are enzymes that reach a maximum complexity degree in bacteria. The Haplosclerida spongeArenosclera brasiliensishosts a complex microbiota and is the source of arenosclerins, alkaloids with cytotoxic and antibacterial activity. In the present investigation, we performed high-throughput sequencing of the ketosynthase (KS) amplicon to investigate the diversity of PKS genes present in the metagenome ofA. brasiliensis. Almost 4,000 ketosynthase reads were recovered, with about 90% annotated automatically as bacterial. A total of 235 bacterial KS contigs was rigorously assembled from this sequence pool and submitted to phylogenetic analysis. A great diversity of six type I PKS groups has been consistently detected in our phylogenetic reconstructions, including a novel andA. brasiliensis-exclusive group. Our study is the first to reveal the diversity of type I PKS genes inA. brasiliensisas well as the potential of its microbiome to serve as a source of new polyketides.

A novel family of nonribosomal peptides modulate collective behavior in Pseudovibrio bacteria isolated from marine sponges

2021 ◽  
Author(s):  
Laura P. Ióca ◽  
Yitao Dai ◽  
Sylvia Kunakom ◽  
Jennifer Diaz-Espinosa ◽  
Aleksej Krunic ◽  
...  
Keyword(s):  
Collective Behavior ◽  
Marine Sponges ◽  
Nonribosomal Peptides

pks63787, a Polyketide Synthase Gene Responsible for the Biosynthesis of Benzenoids in the Medicinal Mushroom Antrodia cinnamomea

2016 ◽  
Vol 79 (6) ◽  
pp. 1485-1491 ◽  
Author(s):  
Po-Wei Yu ◽  
Ya-Chih Chang ◽  
Ruey-Fen Liou ◽  
Tzong-Huei Lee ◽  
Shean-Shong Tzean
Keyword(s):  
Polyketide Synthase ◽  
Medicinal Mushroom ◽  
Antrodia Cinnamomea ◽  
Polyketide Synthase Gene

scholarly journals Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0199110 ◽  
Author(s):  
Yi Wang ◽  
Changan Geng ◽  
Xiaolong Yuan ◽  
Mei Hua ◽  
Fenghua Tian ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Usnic Acid ◽  
Acid Biosynthesis ◽  
Polyketide Synthase Gene

Identification of a polyketide synthase gene ( pksP ) of Aspergillus fumigatus involved in conidial pigment biosynthesis and virulence

1998 ◽  
Vol 187 (2) ◽  
pp. 79-89 ◽  
Author(s):  
K. Langfelder ◽  
Bernhard Jahn ◽  
Heike Gehringer ◽  
Axel Schmidt ◽  
Gerhard Wanner ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Polyketide Synthase ◽  
Polyketide Synthase Gene ◽  
Pigment Biosynthesis

scholarly journals Bacterial tRNA 2′-O-methylation is dynamically regulated under stress conditions and modulates innate immune response

2020 ◽  
Vol 48 (22) ◽  
pp. 12833-12844
Author(s):  
Adeline Galvanin ◽  
Lea-Marie Vogt ◽  
Antonia Grober ◽  
Isabel Freund ◽  
Lilia Ayadi ◽  
...  
Keyword(s):  
Gene Expression Regulation ◽  
Current Knowledge ◽  
Protein Translation ◽  
Stress Conditions ◽  
Transcriptional Level ◽  
Trna Modification ◽  
Rna Modifications ◽  
Swarming Motility ◽  
E Coli ◽  
Response To Stress

Abstract RNA modifications are a well-recognized way of gene expression regulation at the post-transcriptional level. Despite the importance of this level of regulation, current knowledge on modulation of tRNA modification status in response to stress conditions is far from being complete. While it is widely accepted that tRNA modifications are rather dynamic, such variations are mostly assessed in terms of total tRNA, with only a few instances where changes could be traced to single isoacceptor species. Using Escherichia coli as a model system, we explored stress-induced modulation of 2′-O-methylations in tRNAs by RiboMethSeq. This analysis and orthogonal analytical measurements by LC-MS show substantial, but not uniform, increase of the Gm18 level in selected tRNAs under mild bacteriostatic antibiotic stress, while other Nm modifications remain relatively constant. The absence of Gm18 modification in tRNAs leads to moderate alterations in E. coli mRNA transcriptome, but does not affect polysomal association of mRNAs. Interestingly, the subset of motility/chemiotaxis genes is significantly overexpressed in ΔTrmH mutant, this corroborates with increased swarming motility of the mutant strain. The stress-induced increase of tRNA Gm18 level, in turn, reduced immunostimulation properties of bacterial tRNAs, which is concordant with the previous observation that Gm18 is a suppressor of Toll-like receptor 7 (TLR7)-mediated interferon release. This documents an effect of stress induced modulation of tRNA modification that acts outside protein translation.

scholarly journals Morphological description of six species of Suberitida (Porifera: Demospongiae) from the unexplored north-eastern coast of Brazil, with emphasis on two new species

2020 ◽  
Vol 100 (3) ◽  
pp. 389-400
Author(s):  
Humberto F. M. Fortunato ◽  
Thierry Pérez ◽  
Gisele Lôbo-Hajdu
Keyword(s):  
Current Knowledge ◽  
Atlantic Coast ◽  
Marine Sponges ◽  
Eastern Coast ◽  
Morphological Description ◽  
Brazilian Coast ◽  
Northern Coast ◽  
Western Atlantic ◽  
The North ◽  
North Eastern

AbstractThe Order Suberitida is defined as a group of marine sponges without an obvious cortex, a skeleton devoid of microscleres, and with a deletion of a small loop of 15 base pairs in the secondary structure of the 28S rDNA as a molecular synapomorphy. Suberitida comprises three families and 26 genera distributed worldwide, but mostly in temperate and polar waters. Twenty species were reported along the entire Brazilian coast, and although the north-eastern coast of Brazil seems to harbour a rich sponge fauna, our current knowledge is concentrated along the south-eastern Atlantic coast. A survey was implemented along the northern coast of Brazil, and the collection allowed the identification of six species belonging to the Order Suberitida. Two of them are considered new to science: Suberites purpura sp. nov., Hymeniacidon upaonassu sp. nov., and four, Halichondria (Halichondria) marianae Santos, Nascimento & Pinheiro, 2018, Halichondria (H.) melanadocia de Laubenfels, 1936, Suberites aurantiacus (Duchassaing & Michelotti, 1864), and Terpios fugax Duchassaing & Michelotti, 1864, are re-described. Taxonomic comparisons are made for Tropical Western Atlantic species and type species of the four genera. Finally, an identification key for the Western Atlantic Suberites species is provided.

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