scholarly journals Is Vaccination a Viable Method to Control Johne’s Disease Caused by Mycobacterium avium subsp. paratuberculosis? Data from 12 Million Ovine Vaccinations and 7.6 Million Carcass Examinations in New South Wales, Australia from 1999-2009

2021 ◽  
Author(s):  
Ian J. Links ◽  
Laurence J. Denholm ◽  
Marilyn Evers ◽  
Lloyd J. Kingham ◽  
Robert J. Greenstein
Keyword(s):  
Mycobacterium Avium ◽  
New South ◽  
New South Wales ◽  
Johne's Disease ◽  
Farm Management ◽  
Johne’S Disease ◽  
Adult Sheep ◽  
South Wales ◽  
On Farm ◽  
Voluntary Vaccination

AbstractBackgroundMycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease (or paratuberculosis), a chronic wasting disease of ruminants and other animals resulting from granulomatous enteritis. There are increasing concerns that MAP is zoonotic. The prevalence of Johne’s disease is increasing worldwide. In an attempt to control an epidemic of ovine Johne’s disease (OJD) in New South Wales (NSW), a government/industry sponsored voluntary vaccination/on-farm management program commenced in 2000. We report herein an observational study of changes in disease prevalence as vaccination progressed, based on abattoir surveillance data for OJD from 1999 to 2009. We also discuss the epidemiological, policy, regulatory, research, economic and sociological elements that contributed to the development of a mature control program, whose aim was to halt the epidemic spread of OJD in a naïve sheep population.MethodsNSW was divided into areas of “High” (HPA), “Medium” (MPA) and “Low” (LPA) OJD prevalence. A killed whole cell vaccine (Gudair®) was administered to sheep from 2000 to 2009. Trained examiners evaluated the viscera of adult sheep carcasses at slaughter for gross evidence of OJD. MAP infection was confirmed by histopathology.Principal findingsFrom 2000-2009, 12 million vaccine doses were administered in NSW (91%; 10.9 million in the HPA). Many of the vaccinated flocks were suffering > 5% annual mortality in adult sheep, with some individual flocks with 10-15% losses attributable to OJD. A total of 7.6 million carcasses were examined (38%; 2.9 million from the HPA). Overall, 16% of slaughter consignments (sheep consigned to the abattoir from a single vendor) were positive for OJD, of which 94% were from the HPA. In the HPA, the percentage of animals with lesions attributable to OJD at slaughter fell progressively from 2.4% (10,406/432,860) at commencement of vaccination in 2000 to 0.8% (1,573/189,564) by 2009. Herd immunity from vaccination in the HPA was estimated at 70% by 2009. This coincided with a progressive decrease in reports of clinical disease and mortalities in vaccinated flocks.SignificanceWe show a decrease in the prevalence of lesions attributable to OJD in NSW concomitant with initiation of voluntary vaccination, on-farm management plans, abattoir monitoring and feedback of animal prevalence data to sheep producers. We conclude that a target of ≤ 1% regional prevalence of OJD affected sheep at slaughter is achievable using these interventions.

scholarly journals Is vaccination a viable method to control Johne’s disease caused by Mycobacterium avium subsp. paratuberculosis? Data from 12 million ovine vaccinations and 7.6 million carcass examinations in New South Wales, Australia from 1999–2009

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0246411
Author(s):  
Ian J. Links ◽  
Laurence J. Denholm ◽  
Marilyn Evers ◽  
Lloyd J. Kingham ◽  
Robert J. Greenstein
Keyword(s):  
Mycobacterium Avium ◽  
New South ◽  
Control Program ◽  
Johne's Disease ◽  
Farm Management ◽  
Johne’S Disease ◽  
Adult Sheep ◽  
South Wales ◽  
On Farm ◽  
Voluntary Vaccination

Background Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease (or paratuberculosis), a chronic wasting disease of ruminants and other animals resulting from granulomatous enteritis. There are increasing concerns that MAP is zoonotic. The prevalence of Johne’s disease is increasing worldwide. In an attempt to control an epidemic of ovine Johne’s disease (OJD) in New South Wales (NSW), a government/industry sponsored voluntary vaccination/on-farm management program commenced in 2000. We report herein an observational study of changes in disease prevalence as vaccination progressed, based on abattoir surveillance data for OJD from 1999 to 2009. We also discuss the epidemiological, policy, regulatory, research, economic and sociological elements that contributed to the development of a mature control program, whose aim was to halt the epidemic spread of OJD in a naïve sheep population. Methods NSW was divided into areas of “High” (HPA), “Medium” (MPA) and “Low” (LPA) OJD prevalence. A killed whole cell vaccine (Gudair®) was administered to sheep from 2000 to 2009. Trained examiners evaluated the viscera of adult sheep carcasses at slaughter for gross evidence of OJD. MAP infection was confirmed by histopathology. Principal findings From 2000–2009, 12 million vaccine doses were administered in NSW (91%; 10.9 million in the HPA). Many of the vaccinated flocks were suffering > 5% annual mortality in adult sheep, with some individual flocks with 10–15% losses attributable to OJD. A total of 7.6 million carcasses were examined (38%; 2.9 million from the HPA). Overall, 16% of slaughter consignments (sheep consigned to the abattoir from a single vendor) were positive for OJD, of which 94% were from the HPA. In the HPA, the percentage of animals with lesions attributable to OJD at slaughter fell progressively from 2.4% (10,406/432,860) at commencement of vaccination in 2000 to 0.8% (1,573/189,564) by 2009. Herd immunity from vaccination in the HPA was estimated at 70% by 2009, the target commonly espoused for an effective control program based on vaccination. This coincided with a progressive decrease in reports of clinical disease and mortalities in vaccinated flocks. Significance We show a decrease in the prevalence of lesions attributable to OJD in NSW concomitant with initiation of voluntary vaccination, on-farm management plans, abattoir monitoring and feedback of animal prevalence data to sheep producers. We conclude that a target of ≤ 1% regional prevalence of OJD affected sheep at slaughter is achievable using these interventions.

scholarly journals Molecular Epidemiology of Mycobacterium avium subsp.paratuberculosis: IS900 Restriction Fragment Length Polymorphism and IS1311 Polymorphism Analyses of Isolates from Animals and a Human in Australia

2000 ◽  
Vol 38 (9) ◽  
pp. 3240-3248 ◽  
Author(s):  
R. J. Whittington ◽  
A. F. Hope ◽  
D. J. Marshall ◽  
C. A. Taragel ◽  
I. Marsh
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
New South ◽  
New South Wales ◽  
Rflp Analysis ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
South Wales ◽  
Rflp Type ◽  
Restriction Fragment Length

The distribution and prevalence of strains of Mycobacterium avium subsp. paratuberculosis were determined among sheep, cattle, and other species with Johne's disease in Australia. A total of 328 isolates were evaluated from numerous farms in New South Wales, Victoria, Tasmania, and South Australia, Australia. Restriction fragment length polymorphism (RFLP) analysis of genomic DNA usingBstEII and an IS900 probe and IS1311 polymorphism analysis using PCR and restriction endonuclease analysis (PCR-REA) was used to classify isolates as cattle (C) or sheep (S) strains. IS1311 PCR-REA provided similar information to IS900 RFLP analysis but was more useful than RFLP analysis where DNA was degraded or scarce. Twelve IS900 RFLP types were found. Johne's disease in sheep was always due to S strains, while cattle were infected only with C strains. RFLP type S1 was the dominant strain in sheep in New South Wales (97% of isolates) and was the only strain found in sheep from Victoria. Seven RFLP types were present in cattle. RFLP types C3 and C1 were most common (collectively, 85% of isolates), but C1 was not found in New South Wales and C3 was present in dairy cattle but not in beef cattle in Victoria. These differences may be explained by restricted livestock trading patterns between different segments of the cattle industry. Up to five RFLP types were present in some geographic regions in Victoria, while up to three RFLP types were found among cattle on some farms. Individual cattle usually were infected with only one RFLP type, but one animal was infected with both C5 and CU4. Two isolates from goats were C type as were three from alpacas, one from a rhinoceros, and two from a human with Crohn's disease. The prevalences of specific RFLP types in Australia differ from those reported in Europe and elsewhere. Given the existence of geographical and farm enterprise differences in IS900 RFLP type, this technique may be applied selectively to trace the spread of Johne's disease, at least in the cattle industries. As these observations reflect past exposure of livestock to M. avium subsp.paratuberculosis, the monitoring of strains present in animals in Australia is continuing.

Should we manage soil organic carbon in Vertosols in the northern grains region of Australia?

2003 ◽  
Vol 43 (3) ◽  
pp. 261 ◽  
Author(s):  
R. J. Farquharson ◽  
G. D. Schwenke ◽  
J. D. Mullen
Keyword(s):  
Organic Carbon ◽  
Soil Organic Carbon ◽  
New South ◽  
New South Wales ◽  
Soil Health ◽  
Organic Carbon Content ◽  
Continuous Cropping ◽  
South Wales ◽  
On Farm ◽  
Carbon Concentrations

Two issues prompted this paper. The first was the measured soil organic carbon decline in fertile northern Australian soils under continual cropping using traditional management practices. We wanted to see whether it was theoretically possible to maintain or improve soil organic carbon concentrations with modern management recommendations. The second was the debate about use of sustainability indicators for on-farm management, so we looked at soil organic carbon as a potential indicator of soil health and investigated whether it was useful in making on-farm crop decisions. The analytical results indicated first that theoretically the observed decline in soil organic carbon concentrations in some northern cracking clay soils can be halted and reversed under continuous cropping sequences by using best practice management. Second, the results and associated discussion give some support to the use of soil organic carbon as a sustainability indicator for soil health. There was a consistent correlation between crop input decisions (fertilisation, stubble management, tillage), outputs (yield and profits) and outcomes (change in soil organic carbon content) in the short and longer term. And this relationship depended to some extent on whether the existing soil organic carbon status was low, medium or high. A stock dynamics relationship is one where the change in a stock (such as soil organic carbon) through time is related not only to the management decisions made and other random influences (such as climatic effects), but also to the concentration or level of the stock itself in a previous time period. Against such a requirement, soil organic carbon was found to be a reasonable measure. However, the inaccuracy in measuring soil organic carbon in the paddock mitigates the potential benefit shown in this analysis of using soil organic carbon as a sustainability indicator.These results are based on a simulation model (APSIM) calibrated for a cracking clay (Vertosol) soil typical of much of the intensively-cropped slopes and plains region of northern New South Wales and southern Queensland, and need to be interpreted in this light. There are large areas of such soils in north-western New South Wales; however, many of these experience lower rainfalls and plant-available soil water capacities than in this case, and the importance of these characteristics must also be considered.

Low nodulation and nitrogen fixation of mungbean reduce biomass and grain yields

2005 ◽  
Vol 45 (3) ◽  
pp. 269 ◽  
Author(s):  
D. F. Herridge ◽  
M. J. Robertson ◽  
B. Cocks ◽  
M. B. Peoples ◽  
J. F. Holland ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Grain Yield ◽  
New South ◽  
New South Wales ◽  
Soil Nitrate ◽  
Grain Yields ◽  
South Wales ◽  
Negative Effect ◽  
On Farm ◽  
The Relationship

Apparent nodulation failures and associated low grain yields have been reported for commercial mungbean (Vigna radiata) crops in southern Queensland and northern New South Wales. We therefore conducted on-farm surveys of 40 commercial mungbean crops in the region in which symbiotic traits, i.e. nodulation and nitrogen fixation, and biomass and grain yield were monitored. Effects of bradyrhizobial inoculation and inoculation methods on mungbean and soybean (Glycine max) symbiosis and yield were determined in experiments at 3 sites in northern New South Wales. Thirty-four of the 35 mungbean crops assessed for nodulation were nodulated. The relationship between soil nitrate to a depth of 90 cm at sowing and mungbean nodulation was not significant. However, at low-to-moderate soil nitrate levels (<100 kg N/ha), the mean nodule score was 1.6, compared with 0.5 at high (>100 kg N/ha) soil nitrate levels. Soil nitrate had a negative effect on the percentage of mungbean nitrogen derived from nitrogen fixation (%Ndfa). Mean %Ndfa values for soil nitrate levels <50, >50–100 and >100 kg N/ha were 35, 22 and 19% respectively. Grain yields of the surveyed mungbean crops varied from 0.3 to 2.1 t/ha, and were correlated with shoot dry matter. Grain yield was not significantly correlated either with sowing soil nitrate, nodule score or %Ndfa. In the inoculation experiments, mungbean did not nodulate as well as soybean, producing about one-third the number of nodules. Both species responded to inoculation with increased nodulation, although data from one of the sites suggested that responses during early growth of mungbean were not maintained during pod-fill. Effects of inoculation on mungbean %Ndfa were marginal. Average increases were 9%, based on natural 15N abundance, and 6%, based on the ureide method. Soybean %Ndfa, on the other hand, responded strongly to inoculation, with increases of 56 (15N) and 77% (ureide). Inoculation increased mungbean crop N by an average of 10% and grain yield by 6%, compared with responses to fertiliser nitrogen of 31% (crop N) and 10% (grain yield). For soybean, inoculation increased crop nitrogen by 43% and grain yield by 7%, similar to responses to fertiliser nitrogen of 45 (crop N) and 5% (grain yield). These results suggest that inoculated mungbean was N-limited and that inoculation of mungbean using current technology may be somewhat ineffectual. We concluded that low nodulation and nitrogen fixation of commercial mungbean most likely results from the suppressive effects of nitrate and/or insufficient numbers of bradyrhizobia in the soil. When low symbiosis and low soil nitrate are combined, N is likely to limit crop growth, and potentially grain yield. Suggested strategies for improving mungbean nodulation and nitrogen fixation in the northern grains belt include selection of more symbiotically competent plant and bradyrhizobial genotypes and more effective utilisation of established soil populations of mungbean bradyrhizobia.

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