scholarly journals Perceptual Weighting of V1 Spikes Revealed by Optogenetic White Noise Stimulation

2021 ◽  
Author(s):  
Julian R. Day-Cooney ◽  
Jackson J. Cone ◽  
John H.R. Maunsell
Keyword(s):  
White Noise ◽  
Sensory Input ◽  
Perception And Action ◽  
Visual Detection ◽  
Visually Guided ◽  
Neuronal Populations ◽  
Optogenetic Stimulation ◽  
Electrophysiological Recordings ◽  
Perceptual Weighting ◽  
Reverse Correlation Analysis

SummaryDuring visually guided behaviors, mere hundreds of milliseconds can elapse between a sensory input and its associated behavioral response. How spikes occurring at different times are integrated to drive perception and action remains poorly understood. We delivered random trains of optogenetic stimulation (white noise) to excite inhibitory interneurons in V1 of mice while they performed a visual detection task. We then performed a reverse correlation analysis on the optogenetic stimuli to generate a neuronal-behavioral kernel: an unbiased, temporally-precise estimate of how suppression of V1 spiking at different moments around the onset of a visual stimulus affects detection of that stimulus. Electrophysiological recordings enabled us to capture the effects of optogenetic stimuli on V1 responsivity and revealed that the earliest stimulus-evoked spikes are preferentially weighted for guiding behavior. These data demonstrate that white noise optogenetic stimulation is a powerful tool for understanding how patterns of spiking in neuronal populations are decoded in generating perception and action.

scholarly journals Working memory gates visual input to primate prefrontal neurons

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Behrad Noudoost ◽  
Kelsey Lynne Clark ◽  
Tirin Moore
Keyword(s):  
Working Memory ◽  
Prefrontal Cortex ◽  
Sensory Input ◽  
Neural Circuits ◽  
Visual Input ◽  
Visually Guided ◽  
Visual Neurons ◽  
Cortical Inputs ◽  
Behaving Monkeys ◽  
Visual Cortical

Visually guided behavior relies on the integration of sensory input and information held in working memory (WM). Yet it remains unclear how this is accomplished at the level of neural circuits. We studied the direct visual cortical inputs to neurons within a visuomotor area of prefrontal cortex in behaving monkeys. We show that the efficacy of visual input to prefrontal cortex is gated by information held in WM. Surprisingly, visual input to prefrontal neurons was found to target those with both visual and motor properties, rather than preferentially targeting other visual neurons. Furthermore, activity evoked from visual cortex was larger in magnitude, more synchronous, and more rapid, when monkeys remembered locations that matched the location of visual input. These results indicate that WM directly influences the circuitry that transforms visual input into visually guided behavior.

scholarly journals Perceptual detection depends on spike count integration

2019 ◽  
Author(s):  
Jackson J. Cone ◽  
Morgan L. Bade ◽  
Nicolas Y. Masse ◽  
Elizabeth A. Page ◽  
David J. Freedman ◽  
...  
Keyword(s):  
Neural Networks ◽  
Cerebral Cortex ◽  
Visual Cortex ◽  
Recurrent Neural Networks ◽  
Neural Circuit ◽  
Visual Detection ◽  
Spike Count ◽  
Neuronal Responses ◽  
Neuronal Populations ◽  
And Behavior

AbstractWhenever the retinal image changes some neurons in visual cortex increase their rate of firing, while others decrease their rate of firing. Linking specific sets of neuronal responses with perception and behavior is essential for understanding mechanisms of neural circuit computation. We trained mice to perform visual detection tasks and used optogenetic perturbations to increase or decrease neuronal spiking primary visual cortex (V1). Perceptual reports were always enhanced by increments in V1 spike counts and impaired by decrements, even when increments and decrements were delivered to the same neuronal populations. Moreover, detecting changes in cortical activity depended on spike count integration rather than instantaneous changes in spiking. Recurrent neural networks trained in the task similarly relied on increments in neuronal activity when activity was costly. This work clarifies neuronal decoding strategies employed by cerebral cortex to translate cortical spiking into percepts that can be used to guide behavior.

Crustacean Visual Circuits Underlying Behavior

2019 ◽  
Author(s):  
Daniel Tomsic ◽  
Julieta Sztarker
Keyword(s):  
Visual Processing ◽  
Visual Information ◽  
General Structure ◽  
Visual Space ◽  
Experimental Manipulation ◽  
Visually Guided ◽  
Decapod Crustaceans ◽  
Behavioral Experiments ◽  
Electrophysiological Recordings ◽  
Visual Behaviors

Decapod crustaceans, in particular semiterrestrial crabs, are highly visual animals that greatly rely on visual information. Their responsiveness to visual moving stimuli, with behavioral displays that can be easily and reliably elicited in the laboratory, together with their sturdiness for experimental manipulation and the accessibility of their nervous system for intracellular electrophysiological recordings in the intact animal, make decapod crustaceans excellent experimental subjects for investigating the neurobiology of visually guided behaviors. Investigations of crustaceans have elucidated the general structure of their eyes and some of their specializations, the anatomical organization of the main brain areas involved in visual processing and their retinotopic mapping of visual space, and the morphology, physiology, and stimulus feature preferences of a number of well-identified classes of neurons, with emphasis on motion-sensitive elements. This anatomical and physiological knowledge, in connection with results of behavioral experiments in the laboratory and the field, are revealing the neural circuits and computations involved in important visual behaviors, as well as the substrate and mechanisms underlying visual memories in decapod crustaceans.

scholarly journals 0074 Inhibitory Neurons in the Dorsomedial Medulla Promote REM Sleep

SLEEP ◽  
2020 ◽  
Vol 43 (Supplement_1) ◽  
pp. A30-A30
Author(s):  
J Stucynski ◽  
A Schott ◽  
J Baik ◽  
J Hong ◽  
F Weber ◽  
...  
Keyword(s):  
Rem Sleep ◽  
Nrem Sleep ◽  
Inhibitory Neurons ◽  
Brain State ◽  
Sleep Regulation ◽  
Optogenetic Stimulation ◽  
Electrophysiological Recordings ◽  
Stimulation Of

Abstract Introduction The neural circuits controlling rapid eye movement (REM) sleep, and in particular the role of the medulla in regulating this brain state, remains an active area of study. Previous electrophysiological recordings in the dorsomedial medulla (DM) and electrical stimulation experiments suggested an important role of this area in the control of REM sleep. However the identity of the involved neurons and their precise role in REM sleep regulation are still unclear. Methods The properties of DM GAD2 neurons in mice were investigated through stereotaxic injection of CRE-dependent viruses in conjunction with implantation of electrodes for electroencephalogram (EEG) and electromyogram (EMG) recordings and optic fibers. Experiments included in vivo calcium imaging (fiber photometry) across sleep and wake states, optogenetic stimulation of cell bodies, chemogenetic excitation and suppression (DREADDs), and connectivity mapping using viral tracing and optogenetics. Results Imaging the calcium activity of DM GAD2 neurons in vivo indicates that these neurons are most active during REM sleep. Optogenetic stimulation of DM GAD2 neurons reliably triggered transitions into REM sleep from NREM sleep. Consistent with this, chemogenetic activation of DM GAD2 neurons increased the amount of REM sleep while inhibition suppressed its occurrence and enhanced NREM sleep. Anatomical tracing revealed that DM GAD2 neurons project to several areas involved in sleep / wake regulation including the wake-promoting locus coeruleus (LC) and the REM sleep-suppressing ventrolateral periaquaductal gray (vlPAG). Optogenetic activation of axonal projections from DM to LC, and DM to vlPAG was sufficient to induce REM sleep. Conclusion These experiments demonstrate that DM inhibitory neurons expressing GAD2 powerfully promote initiation of REM sleep in mice. These findings further characterize the dorsomedial medulla as a critical structure involved in REM sleep regulation and inform future investigations of the REM sleep circuitry. Support R01 HL149133

Application of Signal Detection Theory to Subliminal and Supraliminal Accessory Stimulation

1969 ◽  
Vol 28 (3) ◽  
pp. 699-704 ◽  
Author(s):  
Marianne F. Zwosta ◽  
Robert Zenhausern
Keyword(s):  
Signal Detection ◽  
White Noise ◽  
Signal Detection Theory ◽  
Visual Detection ◽  
Detection Theory ◽  
Detection Task ◽  
Subliminal Stimulation

The effect of subliminal and supraliminal accessory stimulation (white noise) on a visual detection task was determined through the use of the Theory of Signal Detection (SDT). Both the most extreme level of subliminal stimulation (–15 db) and the most extreme level of supraliminal stimulation (+15 db) resulted in the greatest increase in sensitivity ( d′) but neither had any effect on S's criteria (Beta).

Potters Make Shorter Pots Under Conditions of Reduced Sensory Input

Perception ◽  
2018 ◽  
Vol 47 (8) ◽  
pp. 860-872
Author(s):  
Mounia Ziat ◽  
Min Park ◽  
Brian Kakas ◽  
David A. Rosenbaum
Keyword(s):  
Visual Feedback ◽  
Sensory Feedback ◽  
Sensory Input ◽  
Perception And Action ◽  
Behavioral Research ◽  
Control Hypothesis ◽  
Clay Pots ◽  
Do So

Although people have made clay pots for millennia, little behavioral research has explored how they do so. We were specifically interested in potters’ use of auditory, haptic, and visual feedback. We asked what would happen if one or two of these sources of feedback were removed and potters tried to create pots of a given height, stopping when they thought they had reached that height. We asked students in a pottery class to build simple clay vessels either when they had full sensory feedback (in the control condition for all participants) or when they had reduced input from one modality (in Experiment 1) or two modalities (in Experiment 2). Participants were asked to stop building the vessels when they thought the vessels were 5 in. high. We found that participants produced shorter vessels when one or more forms of sensory feedback was reduced. The degree of shortening did not depend on the type or number of reduced sensory channels. The results are consistent with a control hypothesis where potters must have learned how to use sensory feedback from the modalities to help them control their ceramic creations. The results help highlight the importance of the intimate connections between perception and action.

scholarly journals Direct intertectal inputs are an integral component of the bilateral sensorimotor circuit for behavior in Xenopus tadpoles

2018 ◽  
Vol 119 (5) ◽  
pp. 1947-1961 ◽  
Author(s):  
Abigail C. Gambrill ◽  
Regina L. Faulkner ◽  
Hollis T. Cline
Keyword(s):  
Optic Tectum ◽  
Visual Information ◽  
Sensory Input ◽  
Visual Experience ◽  
Visually Guided ◽  
Visuomotor Behavior ◽  
Retinal Projections ◽  
Integral Component ◽  
And Function ◽  
Circuit Function

The circuit controlling visually guided behavior in nonmammalian vertebrates, such as Xenopus tadpoles, includes retinal projections to the contralateral optic tectum, where visual information is processed, and tectal motor outputs projecting ipsilaterally to hindbrain and spinal cord. Tadpoles have an intertectal commissure whose function is unknown, but it might transfer information between the tectal lobes. Differences in visual experience between the two eyes have profound effects on the development and function of visual circuits in animals with binocular vision, but the effects on animals with fully crossed retinal projections are not clear. We tested the effect of monocular visual experience on the visuomotor circuit in Xenopus tadpoles. We show that cutting the intertectal commissure or providing visual experience to one eye (monocular visual experience) is sufficient to disrupt tectally mediated visual avoidance behavior. Monocular visual experience induces asymmetry in tectal circuit activity across the midline. Repeated exposure to monocular visual experience drives maturation of the stimulated retinotectal synapses, seen as increased AMPA-to-NMDA ratios, induces synaptic plasticity in intertectal synaptic connections, and induces bilaterally asymmetric changes in the tectal excitation-to-inhibition ratio (E/I). We show that unilateral expression of peptides that interfere with AMPA or GABAA receptor trafficking alters E/I in the transfected tectum and is sufficient to degrade visuomotor behavior. Our study demonstrates that monocular visual experience in animals with fully crossed visual systems produces asymmetric circuit function across the midline and degrades visuomotor behavior. The data further suggest that intertectal inputs are an integral component of a bilateral visuomotor circuit critical for behavior. NEW & NOTEWORTHY The developing optic tectum of Xenopus tadpoles represents a unique circuit in which laterally positioned eyes provide sensory input to a circuit that is transiently monocular, but which will be binocular in the animal’s adulthood. We challenge the idea that the two lobes of tadpole optic tectum function independently by testing the requirement of interhemispheric communication and demonstrate that unbalanced sensory input can induce structural and functional plasticity in the tectum sufficient to disrupt function.

scholarly journals EEG Frequency Tagging to Dissociate the Cortical Responses to Nociceptive and Nonnociceptive Stimuli

2014 ◽  
Vol 26 (10) ◽  
pp. 2262-2274 ◽  
Author(s):  
Elisabeth Colon ◽  
Valéry Legrain ◽  
André Mouraux
Keyword(s):  
Sensory Input ◽  
Detection Task ◽  
Cortical Processing ◽  
Focus Attention ◽  
Top Down ◽  
Neuronal Populations ◽  
Cortical Responses ◽  
Somatosensory Input ◽  
Nociceptive Input ◽  
Target Detection Task

Whether the cortical processing of nociceptive input relies on the activity of nociceptive-specific neurons or whether it relies on the activity of neurons also involved in processing nonnociceptive sensory input remains a matter of debate. Here, we combined EEG “frequency tagging” of steady-state evoked potentials (SS-EPs) with an intermodal selective attention paradigm to test whether the cortical processing of nociceptive input relies on nociceptive-specific neuronal populations that can be selectively modulated by top–down attention. Trains of nociceptive and vibrotactile stimuli (Experiment 1) and trains of nociceptive and visual stimuli (Experiment 2) were applied concomitantly to the same hand, thus eliciting nociceptive, vibrotactile, and visual SS-EPs. In each experiment, a target detection task was used to focus attention toward one of the two concurrent streams of sensory input. We found that selectively attending to nociceptive or vibrotactile somatosensory input indistinctly enhances the magnitude of nociceptive and vibrotactile SS-EPs, whereas selectively attending to nociceptive or visual input independently enhances the magnitude of the SS-EP elicited by the attended sensory input. This differential effect indicates that the processing of nociceptive input involves neuronal populations also involved in the processing of touch, but distinct from the neuronal populations involved in vision.

scholarly journals Visuo-vestibular gaze control - conserved subcortical processing

2021 ◽  
Author(s):  
Tobias Wibble ◽  
Tony Pansell ◽  
Sten Grillner ◽  
Juan Perez-Fernandez
Keyword(s):  
Visual Stimulation ◽  
Vestibular Stimulation ◽  
Visually Guided ◽  
Movement Response ◽  
Gaze Stabilization ◽  
Electrophysiological Recordings ◽  
Sensory Modalities ◽  
Image Blurring ◽  
Multisensory Information ◽  
Living Group

Gaze stabilization compensates for movements of the head or external environment to minimize image blurring, which is critical for visually-guided behaviors. Multisensory information is used to stabilize the visual scene on the retina via the vestibulo-ocular (VOR) and optokinetic (OKR) reflexes. While the organization of neuronal circuits underlying VOR is well described across vertebrates, less is known about the contribution and evolutionary origin of the OKR circuits. Moreover, the integration of these two sensory modalities is still poorly understood. Here, we developed a novel experimental model, the isolated lamprey eye-brain-labyrinth preparation, to analyze the neuronal pathways underlying visuo-vestibular integration which allowed electrophysiological recordings while applying vestibular stimulation using a moving platform, coordinated with visual stimulation via two screens. We show that lampreys exhibit robust visuo-vestibular integration, with optokinetic information processed in the pretectum and integrated with vestibular inputs at several subcortical levels. The enhanced eye movement response to multimodal stimulation favored the vestibular response at increased velocities. The optokinetic signals can be downregulated from tectum. Additionally, saccades are present in the form of nystagmus. The lamprey represents the oldest living group of vertebrates, thus all basic components of the visuo-vestibular control of gaze were present already at the dawn of vertebrate evolution.

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