On the Analysis of Transcriptional Noise From RNA-sequencing Data
Keyword(s):
Rna Seq
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RNA-sequencing (RNA-seq) has revolutionized our understanding of molecular and cellular biology. A central cornerstone in the analysis of RNA-seq is the bioinformatic tools that quantify the data. To evaluate the efficacy of these tools, scientists rely heavily on simulation of RNA-seq. Recently Varabyou et al. took simulation of RNA-seq data to the next level by providing simulated data, that includes simulation of transcriptional noise. While this represents a significant step forward in our ability to perform realistic benchmarks of RNA-seq tools, the data provided by Varabyou et al. need refinement. In the following, I suggest a few improvements with a specific focus on splicing noise.