scholarly journals The Effects of Phenolic Compounds Isolated from Chinese Herbal Medicine Rhodiola Rosea on Preventing Photoaging of Keratinocytes

2021 ◽  
Author(s):  
Wu Han Toh ◽  
Jude C. Clapper ◽  
Cheng-Chang Hsu ◽  
Chun-Bing Chen ◽  
Hua-en Lee ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
High Performance ◽  
Telomerase Activity ◽  
Rhodiola Rosea ◽  
Hacat Cells ◽  
Uvb Radiation ◽  
Aging Effects ◽  
Active Ingredients ◽  
Chinese Herbal

Among the many health benefits Chinese herbal medicine (CHM) presents, anti-aging is of special interest. Reported to possess anti-aging effects, the CHM Rhodiola rosea, known colloquially as the golden root, has been widely incorporated in various drinks, daily supplements, and even cosmetics. This study investigates the effects of commercial Rhodiola extracts on preventing UV-induced photoaging of the skin and correlates such effects with the composition of active ingredients in the extracts. To simulate the photoaging process, drug treated HaCaT cells were exposed to UVA and UVB radiation. The pharmacological anti-aging effects of Rhodiola extracts were evaluated qualitatively and quantitatively through confocal immunofluorescence images with γ-H2A.X marker and telomerase activity assay (Telo TAGGG Telomerase PCR-ELISA). Preparatory thin layer chromatography and high-performance liquid chromatography were performed to isolate and quantify active ingredients. Cultured HaCaT cells showed morphological change after exposure to both UVA (>15.0 J) and UVB (>2000 mJ). The photoaging of keratinocytes was rescued by pretreating cells with Rhodiola extracts as well as salidroside and rosavin active ingredients (P < 0.05). Rhodiola-treated cells were characterized by increased telomerase activity and fewer γ-H2A.X foci compared to that of the control. Extracts with better preventative effects contained higher salidroside and rosavin content. The findings in this study reaffirm Rhodiola's efficacy as an anti-aging remedy and provide a basis for CHM's integration into the mainstream of global healthcare.

Simultaneous quantification of six sesquiterpene lactones and a flavonoid in the whole life stage of Inula salsoloides by high performance liquid chromatography

2016 ◽  
Vol 8 (17) ◽  
pp. 3587-3591 ◽  
Author(s):  
Lu Bai ◽  
Mi Jiang ◽  
Sen Guo ◽  
Qingchao Liu ◽  
Xinxin Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
High Performance ◽  
Sesquiterpene Lactones ◽  
Life Stage ◽  
Bioactive Substances ◽  
Simultaneous Quantification ◽  
Chinese Herbal

Sesquiterpene lactones and flavonoids are the most bioactive substances in Chinese herbal medicine Inula salsoloides.

scholarly journals Effect of Active Ingredients of Chinese Herbal Medicine on the Rejuvenation of Healthy Aging: Focus on Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-18
Author(s):  
Chen Wang ◽  
Shuang Ling ◽  
Jin-Wen Xu
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Herbal Medicines ◽  
Stem Cell Differentiation ◽  
Chinese Herbs ◽  
Active Ingredients ◽  
Chinese Herbal Medicines ◽  
Chinese Herbal

Stem cells (SCs) are special types of cells with the ability of self-renewal and multidirectional differentiation. As the organism ages, the ability to maintain homeostasis and regeneration deteriorates and the number and activity of stem cells decline. Theoretically, the restoration of stem cells might reverse aging. However, due to their own aging, donor-derived immune rejection, and difficulties in stem cell differentiation control, a series of problems need to be solved to realize the potential for clinical application of stem cells. Chinese herbal medicine is a nature drug library which is suitable for the long-term treatment of aging-related diseases. Modern pharmacological studies have revealed that many active ingredients of Chinese herbal medicines with the effect of promoting stem cells growth and differentiation mainly belong to “reinforcing herbs.” In recent years, exploration of natural active ingredients from Chinese herbal medicines for delaying aging, improving the stem cell microenvironment, and promoting the proliferation and differentiation of endogenous stem cells has attracted substantial attention. This article will focus on active ingredients from Chinese herbs-mediated differentiation of stem cells into particular cell type, like neural cells, endothelial cells, cardiomyocytes, and osteoblasts. We will also discuss the effects of these small molecules on Wnt, Sonic Hedgehog, Notch, eNOS-cGMP, and MAP kinase signal transduction pathways, as well as reveal the role of estrogen receptor α and PPAR γ on selectively promoting or inhibiting stem cells differentiation. This review will provide new insights into the health aging strategies of active ingredients in Chinese herbal medicine in regenerative medicine.

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