scholarly journals High-affinity iron and calcium transport pathways are involved in U(VI) uptake in the budding yeast Saccharomyces cerevisiae

2021 ◽  
Author(s):  
Benoît REVEL ◽  
Patrice CATTY ◽  
Stéphane RAVANEL ◽  
Jacques BOURGUIGNON ◽  
Claude ALBAN
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Living Organism ◽  
Functional Expression ◽  
Model Systems ◽  
Rapid Screening ◽  
Unicellular Eukaryote ◽  
Yeast Saccharomyces Cerevisiae ◽  
Transport Pathways ◽  
Living Organisms ◽  
Calcium Iron

Uranium (U) is a naturally-occurring radionuclide toxic for living organisms that can take it up. To date, the mechanisms of U uptake are far from being understood. Here, we used the yeast Saccharomyces cerevisiae as a unicellular eukaryote model to identify U assimilation pathways. Thus, we have identified, for the first time, transport machineries capable of transporting U in a living organism. First, we evidenced a metabolism-dependent U transport in yeast. Then, competition experiments with essential metals allowed us to identify calcium, iron and copper entry pathways as potential routes for U uptake. The analysis of various metal transport mutants revealed that mid1Δ, cch1Δ and ftr1Δ mutants, affected in calcium (Mid1/Cch1 channel) and Fe(III) (Ftr1/Fet3 complex) transport, respectively, exhibited highly reduced U uptake rates and accumulation, demonstrating the implication of these import systems in U uptake. Finally, expression of the Mid1 gene into the mid1Δ mutant restored U uptake levels of the wild type strain, underscoring the central role of the Mid1/Cch1 calcium channel in U absorption process in yeast. Our results also open up the opportunity for rapid screening of U-transporter candidates by functional expression in yeast, before their validation in more complex higher eukaryote model systems.

scholarly journals Enzymes of yeast polyphosphate metabolism: structure, enzymology and biological roles

2016 ◽  
Vol 44 (1) ◽  
pp. 234-239 ◽  
Author(s):  
Rūta Gerasimaitė ◽  
Andreas Mayer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Blood Coagulation ◽  
Bone Mineralization ◽  
Inorganic Polyphosphate ◽  
Yeast Saccharomyces Cerevisiae ◽  
Future Directions ◽  
Short Overview ◽  
Metabolism Enzymes ◽  
Living Organisms ◽  
Polyphosphate Metabolism

Inorganic polyphosphate (polyP) is found in all living organisms. The known polyP functions in eukaryotes range from osmoregulation and virulence in parasitic protozoa to modulating blood coagulation, inflammation, bone mineralization and cellular signalling in mammals. However mechanisms of regulation and even the identity of involved proteins in many cases remain obscure. Most of the insights obtained so far stem from studies in the yeast Saccharomyces cerevisiae. Here, we provide a short overview of the properties and functions of known yeast polyP metabolism enzymes and discuss future directions for polyP research.

scholarly journals Preface

1995 ◽  
Vol 347 (1319) ◽  
pp. 3-3
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Molecular Mechanisms ◽  
Model Systems ◽  
Biochemical Pathways ◽  
Major Threat ◽  
Environmental Agents ◽  
Living Organisms ◽  
New Research ◽  
Micro Organisms

Genomic instability is a major threat to living organisms. To counteract the damaging effects posed by endogenous and environmental agents, such as chemicals or radiation, micro-organisms devote several percent of their genome to encode proteins that function in the repair and recombination of DNA. For many years, a relatively small group of scientists have carefully delineated the molecular mechanisms of these repair processes, using the simplest model systems available, namely Escherichia coli and Saccharomyces cerevisiae . These studies, which until recently had only moderate impact outside of the field, now provide the cornerstone for exciting new research into analogous processes in hum an cells. The reason for this is the revelation that the biochemical pathways for the accurate replication, repair and recombination of DNA have been conserved through evolution.

scholarly journals Vacuole Biogenesis in Saccharomyces cerevisiae: Protein Transport Pathways to the Yeast Vacuole

1998 ◽  
Vol 62 (1) ◽  
pp. 230-247 ◽  
Author(s):  
Nia J. Bryant ◽  
Tom H. Stevens
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Delivery ◽  
Secretory Pathway ◽  
Protein Transport ◽  
Transport Pathway ◽  
Yeast Saccharomyces Cerevisiae ◽  
Transport Pathways ◽  
Yeast Vacuole ◽  
Lysosome Biogenesis ◽  
Vacuolar Protein

SUMMARY Delivery of proteins to the vacuole of the yeast Saccharomyces cerevisiae provides an excellent model system in which to study vacuole and lysosome biogenesis and membrane traffic. This organelle receives proteins from a number of different routes, including proteins sorted away from the secretory pathway at the Golgi apparatus and endocytic traffic arising from the plasma membrane. Genetic analysis has revealed at least 60 genes involved in vacuolar protein sorting, numerous components of a novel cytoplasm-to-vacuole transport pathway, and a large number of proteins required for autophagy. Cell biological and biochemical studies have provided important molecular insights into the various protein delivery pathways to the yeast vacuole. This review describes the various pathways to the vacuole and illustrates how they are related to one another in the vacuolar network of S. cerevisiae.

Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae

1987 ◽  
Vol 7 (6) ◽  
pp. 2087-2096
Author(s):  
B Sauer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Functional Expression ◽  
Yeast Saccharomyces Cerevisiae ◽  
Efficient Manner ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Leu2 Gene ◽  
Recombination System ◽  
A Site ◽  
Site Specific Recombination System

The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recombinase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.

scholarly journals Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae.

1987 ◽  
Vol 7 (6) ◽  
pp. 2087-2096 ◽  
Author(s):  
B Sauer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Functional Expression ◽  
Yeast Saccharomyces Cerevisiae ◽  
Efficient Manner ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Leu2 Gene ◽  
Recombination System ◽  
A Site ◽  
Site Specific Recombination System

The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recombinase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.

Bioaccumulation of Chromium(III) from Aqueous Solutions of a Leather Wastewater Treatment Plant by Saccharomyces cerevisiae Yeast

2020 ◽  
Vol 115 (11) ◽  
pp. 413-417
Author(s):  
Patrizia Janković ◽  
Renos Spinosi ◽  
Anna Bacardit
Keyword(s):  
Heavy Metals ◽  
Saccharomyces Cerevisiae ◽  
Wastewater Treatment ◽  
Human Life ◽  
Treatment Plant ◽  
Exposure Period ◽  
Residual Water ◽  
Yeast Saccharomyces Cerevisiae ◽  
Removal Of Heavy Metals ◽  
Living Organisms

With many industries discharging heavy metals into natural water resources, heavy metals have been found to accumulate in various living organisms which can ultimately threaten human life and pose a big threat to the environment. Thus, in the pursuit of a solution to the above mentioned problem, bioaccumulation has emerged as an interesting option for the removal of heavy metals from wastewater. In this paper, the effectiveness of the yeast Saccharomyces cerevisiae in the bioaccumulation of Cr3+ has been tested. Also, different factors influencing Cr3+ uptake have been discussed.  This work has demonstrated that Saccharomyces cerevisiae is an effective Cr3+ biosorbent for tannery wastewater. The conditions of use of this yeast to achieve optimal chromium (III) absorption are: i) when a growth of the biosorbent equivalent to a similar concentration of Cr3+ is obtained, which contains the residual water that needs to be treated; ii) the smaller the biosorbent is the better the biosorption; iii) the uptake of Cr3+ is more efficient when no extra growth medium is added to the wastewater; iv) the longer the exposure period of the yeast to Cr3+ , the bigger the Cr3+ reduction. Since Saccharomyces cerevisiae is an inexpensive, readily available source of biomass, this discovery could be of great use for a low-budget and efficient wastewater treatment system

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