scholarly journals Dual-lipid metabolomics revealed the communication of MTB or MB with Bovine alveolar macrophages in lipid metabolisms

2021 ◽  
Author(s):  
Weifeng Gao ◽  
Yurong Cai ◽  
Gang Zhang ◽  
Xiaoping Wang ◽  
Juan Wang ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Mycobacterium Tuberculosis ◽  
Immune Responses ◽  
Lipid Composition ◽  
Mycobacterium Tuberculosis Complex ◽  
Tuberculosis Complex ◽  
Host Macrophage ◽  
Causative Agents ◽  
Bovine Alveolar Macrophage ◽  
Fatty Acyls

M. tuberculosis(MTB) and M. bovis(MB) of the Mycobacterium tuberculosis complex (MTBC) are the causative agents of the notorious infectious disease tuberculosis(TB) in a range of mammals, including cattle and human. The lipid composition of MTB/MB performed imperative function as invading host macrophage. In the present study, a dual-lipid metabolomics were used to elucidate the differences in lipid composition of MTB and MB and the different responses in lipid metabolisms of bovine alveolar macrophage challenged by MTB/MB. The lipid metabolomics of MTB and MB indicated that there were significant differences in lipid composition of both bacteria that the level of various lipids belonged to Glycerophospholipids, Sterol Lipids, Fatty Acyls and Polyketides exhibited differences between MTB and MB. Meanwhile, both MTB and MB with different lipid composition could invoked different responses in lipid metabolisms of the host macrophage. MTB infection mainly induced the increase in content of Polyketides and Glycerophospholipids in macrophages, whereas MB infection induced the level of Glycerophospholipids and Sterol Lipids of macrophages. Furthermore, we identified TAG 13:0-18:5-18:5 of MTB and PC(16:1(9E)/0:0), PI(20:2(11Z,14Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)), 4,6-Decadiyn-1-ol isovalerate and LacCer(d18:1/24:1(15Z)) of MB caused the different variations in lipid metabolisms of macrophage following MTB/MB attacks, respectively. Finally, we proposed MTB and MB with different lipid compositions could successfully colonize in macrophage by different mechanisms that MTB could promote the formation of foam cells of macrophage for its colonization and development, while MB mainly through suppressing the macrophage autophagy to escape the immune responses of host.

scholarly journals Revised Interpretation of the Hain Lifescience GenoType MTBC To Differentiate Mycobacterium canettii and Members of the Mycobacterium tuberculosis Complex

2019 ◽  
Vol 63 (6) ◽  
Author(s):  
Chloé Loiseau ◽  
Daniela Brites ◽  
Irmgard Moser ◽  
Francesc Coll ◽  
Christine Pourcel ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
In Silico ◽  
Mycobacterium Tuberculosis Complex ◽  
Tuberculosis Complex ◽  
Content Type ◽  
Causative Agents ◽  
Mycobacterium Africanum

ABSTRACT Using 894 phylogenetically diverse genomes of the Mycobacterium tuberculosis complex (MTBC), we simulated in silico the ability of the Hain Lifescience GenoType MTBC assay to differentiate the causative agents of tuberculosis. Here, we propose a revised interpretation of this assay to reflect its strengths (e.g., it can distinguish some strains of Mycobacterium canettii and variants of Mycobacterium bovis that are not intrinsically resistant to pyrazinamide) and limitations (e.g., Mycobacterium orygis cannot be differentiated from Mycobacterium africanum).

scholarly journals Tuberculosis in Antelopes in a Zoo in Poland – Problem of Public Health

2015 ◽  
Vol 64 (4) ◽  
pp. 395-397 ◽  
Author(s):  
Monika Krajewska ◽  
Michał Załuski ◽  
Anna Zabost ◽  
Blanka Orłowska ◽  
Ewa Augustynowicz-Kopeć ◽  
...  
Keyword(s):  
Public Health ◽  
Infectious Disease ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Wild Animals ◽  
Tuberculosis Complex ◽  
Mycobacterium Caprae ◽  
In Captivity

Bovine tuberculosis is an infectious disease that occurs in many species of both domestic and wild animals, as well as those held in captivity. The etiological factor is the acid resistant bacillus (Mycobacterium bovis or Mycobacterium caprae), which is characterized by the major pathogenicity among mycobacteria belonging to the Mycobacterium tuberculosis complex. The material from 8 antelopes from the zoo, suspected for tuberculosis were examined, and M. bovis strains were isolated from 6 of them. The spoligotyping method showing spoligo pattern 676763777777600. In Poland, this spoligotype has not been observed so far.

scholarly journals Spatial and Temporal Distribution of Mycobacterium tuberculosis Complex Infection in Eurasian Badger (Meles meles) and Cattle in Asturias, Spain

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1294
Author(s):  
Cristina Blanco Blanco Vázquez ◽  
Thiago Doria Barral ◽  
Beatriz Romero ◽  
Manuel Queipo ◽  
Isabel Merediz ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Road Traffic ◽  
Mycobacterium Tuberculosis Complex ◽  
Temporal Distribution ◽  
Meles Meles ◽  
Interspecies Transmission ◽  
Tuberculosis Complex ◽  
Serum Samples ◽  
Study Objective ◽  
Infection Pressure

The present work investigated the prevalence, spatial distribution, and temporal distribution of tuberculosis (TB) in free-ranging Eurasian badgers (Meles meles) and cattle in Asturias (Atlantic Spain) during a 13-year follow-up. The study objective was to assess the role of badgers as a TB reservoir for cattle and other sympatric wild species in the region. Between 2008 and 2020, 673 badgers (98 trapped and 575 killed in road traffic accidents) in Asturias were necropsied, and their tissue samples were cultured for the Mycobacterium tuberculosis complex (MTC) isolation. Serum samples were tested in an in-house indirect P22 ELISA to detect antibodies against the MTC. In parallel, data on MTC isolation and single intradermal tuberculin test results were extracted for cattle that were tested and culled as part of the Spanish National Program for the Eradication of Bovine TB. A total of 27/639 badgers (4.23%) were positive for MTC based on bacterial isolation, while 160/673 badgers (23.77%) were found to be positive with the P22 ELISA. The rate of seropositivity was higher among adult badgers than subadults. Badger TB status was spatially and temporally associated with cattle TB status. Our results cannot determine the direction of possible interspecies transmission, but they are consistent with the idea that the two hosts may exert infection pressure on each other. This study highlights the importance of the wildlife monitoring of infection and disease during epidemiological interventions in order to optimize outcomes.

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