scholarly journals CV-containing vesicle budding from chloroplast inner envelope membrane is mediated by clathrin but inhibited by GAPC

2021 ◽  
Author(s):  
Ting Pan ◽  
Yangxuan Liu ◽  
Chengcheng Ling ◽  
Yuying Tang ◽  
Wei Tang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Abiotic Stress ◽  
Water Stress ◽  
Stress Response ◽  
Eukaryotic Cells ◽  
Envelope Membrane ◽  
Vesicle Budding ◽  
Cargo Transport ◽  
Membrane Defects ◽  
Inner Envelope

AbstractClathrin-mediated vesicular formation and trafficking are highly conserved in eukaryotic cells and are responsible for molecular cargo transport and signal transduction among organelles. It remains largely unknown whether clathrin-coated vesicles can be generated from chloroplasts. CHLOROPLAST VESICULATION (CV)-containing vesicles (CVVs) generate from chloroplasts and mediate chloroplast degradation under abiotic stress. In this study, we showed that CV interacted with the clathrin heavy chain (CHC) and induced vesicle budding from the chloroplast inner envelope membrane. Defects on CHC2 and the dynamin-encoding DRP1A gene affected CVV budding and releasing from chloroplast. CHC2 is also required for CV-induced chloroplast degradation and hypersensitivity to water stress. Moreover, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAPC) interacts with CV and impairs the CV-CHC2 interaction. GAPC1 overexpression inhibited CV-mediated chloroplast degradation and hypersensitivity to water stress. CV silencing alleviated the hypersensitivity of gapc1gapc2 plant to water stress. Together, our work revealed a pathway of clathrin-assisted CVV budding from the chloroplast inner envelope membrane, which mediated the stress-induced chloroplast degradation and stress response.

scholarly journals Gene Expression and Signal Transduction in Water-Stress Response

1997 ◽  
Vol 115 (2) ◽  
pp. 327-334 ◽  
Author(s):  
K. Shinozaki ◽  
K. Yamaguchi-Shinozaki
Keyword(s):  
Gene Expression ◽  
Signal Transduction ◽  
Water Stress ◽  
Stress Response

scholarly journals Humic acids trigger the weak acids stress response in maize seedlings

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Daiane Carvalho Baía ◽  
Fábio L. Olivares ◽  
Daniel B. Zandonadi ◽  
Cleiton de Paula Soares ◽  
Riccardo Spaccini ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Intracellular Ph ◽  
Humic Acids ◽  
Abiotic Stress Tolerance ◽  
Molecular Response ◽  
Maize Seedlings ◽  
Weak Acids ◽  
Acetoxymethyl Ester ◽  
Chemical Priming

Abstract Background Plants primed by humic acids showed physiological and molecular response against different abiotic stresses without the presence of stressor agents (salinity, drought, heavy metal toxicity). It is plausible that humic acids themselves can act as chemical priming substances in plants. We hypothesized that humic acids can trigger the weak acids stress response in cell plants acidifying the cytosol and thus eliciting the transduction signalling response cascade. Methods The dose–response curves of maize seedlings roots with different concentrations of humic, acetic and salicylic acids determined the most active and inhibitory concentration. These data were further used to evaluate changes on intracellular pH using BCECF-AM probe (2,7-bis(2-carboxyethyl)-5(and 6)-carboxyfluorescein, acetoxymethyl ester) and differential transcription level of genes related to weak stress response in plants by qPCR real time. Results Humic acids like short chain organic acids decrease the intracellular pH showed by the increased fluorescence of BCECF probe. The drop in cytosolic pH promoted by humic acids was not transient. We observed a high level of protein kinases related to cell energy-sensing and transcription factors associated to transduction of stress signalling. Conclusion The humic acids can be considered as a chemical priming agent, since in the appropriate concentration they can induce the typical plant abiotic stress response of weak acids inducing plant acclimation and enhancing the abiotic stress tolerance.

scholarly journals Characterization of Atypical Protein Tyrosine Kinase (PTK) Genes and Their Role in Abiotic Stress Response in Rice

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 664
Author(s):  
Allimuthu Elangovan ◽  
Monika Dalal ◽  
Gopinathan Kumar Krishna ◽  
Sellathdurai Devika ◽  
Ranjeet Ranjan Kumar ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Tyrosine Kinase ◽  
Tyrosine Phosphorylation ◽  
Rice Genome ◽  
Kinase Domain ◽  
Tyrosine Kinase Domain ◽  
Protein Tyrosine ◽  
Dual Specificity ◽  
Submergence Stress

Tyrosine phosphorylation constitutes up to 5% of the total phophoproteome. However, only limited studies are available on protein tyrosine kinases (PTKs) that catalyze protein tyrosine phosphorylation in plants. In this study, domain analysis of the 27 annotated PTK genes in rice genome led to the identification of 18 PTKs with tyrosine kinase domain. The kinase domain of rice PTKs shared high homology with that of dual specificity kinase BRASSINOSTEROID-INSENSITIVE 1 (BRI1) of Arabidopsis. In phylogenetic analysis, rice PTKs clustered with receptor-like cytoplasmic kinases-VII (RLCKs-VII) of Arabidopsis. mRNAseq analysis using Genevestigator revealed that rice PTKs except PTK9 and PTK16 express at moderate to high level in most tissues. PTK16 expression was highly abundant in panicle at flowering stage. mRNAseq data analysis led to the identification of drought, heat, salt, and submergence stress regulated PTK genes in rice. PTK14 was upregulated under all stresses. qRT-PCR analysis also showed that all PTKs except PTK10 were significantly upregulated in root under osmotic stress. Tissue specificity and abiotic stress mediated differential regulation of PTKs suggest their potential role in development and stress response of rice. The candidate dual specificity PTKs identified in this study paves way for molecular analysis of tyrosine phosphorylation in rice.

scholarly journals The Raf-like kinase ILK1 and the high affinity K+ transporter HAK5 are required for Innate Immunity and Abiotic Stress Response

2016 ◽  
pp. pp.00035.2016 ◽  
Author(s):  
Elizabeth Kalinda Brauer ◽  
Nagib Ahsan ◽  
Renee Dale ◽  
Naohiro Kato ◽  
Alison E Coluccio ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Abiotic Stress ◽  
Stress Response ◽  
Abiotic Stress Response ◽  
High Affinity

Difference of MreBCD Complex Interactome in Salmonella Typhimurium ST19 and ST213 Genotypes on Pathogenesis and Stress Response Pathways

2021 ◽  
Vol 22 ◽  
Author(s):  
Reyna Cristina Zepeda-Gurrola ◽  
Gerardo Vázquez-Marrufo ◽  
Xianwu Guo ◽  
Isabel Cristina Rodríguez-Luna ◽  
Alejandro Sánchez-Varela ◽  
...  
Keyword(s):  
Stress Response ◽  
Bacterial Virulence ◽  
Eukaryotic Cells ◽  
Interaction Patterns ◽  
High Infection Rate ◽  
Differential Interaction ◽  
Sequence Variations ◽  
High Infection ◽  
Underlying Mechanisms

: Salmonella enterica is the etiological agent of salmonellosis, with a high infection rate worldwide. In Mexico, ST213 genotype of S. enterica ser. Typhimurium is displacing the ancestral ST19 genotype. Bacterial cytoskeleton protein complex MreBCD play an important role in S. enterica pathogenesis, but underlying mechanisms are unknown. In this study, 106 interactions among MreBCD and 15 proteins from S. Typhimurium Pathogenicity Islands 1 (SP-I) and 2 (SP-2) involved in both bacterial virulence and stress response were predicted in ST213 and ST19 genotypes, of which 12 interactions were confirmed in vitro. In addition, gene cluster analysis in 100 S. Typhimurium genomes was performed for these genes. The in silico and in vitro results showed a novel MreBCD interactome involved in the regulation of pathogenesis and stress response through interactions with virulence factors located at SPI-1 and SPI-2. Furthermore, both pseudogene presence and sequence variations in four tested proteins between genotypes resulted in differential interaction patterns that are involved in Salmonella motility and survival in eukaryotic cells, which could explain replacement of ST19 by ST213 in Mexico.

scholarly journals Arabidopsis Tic40 Expression in Tobacco Chloroplasts Results in Massive Proliferation of the Inner Envelope Membrane and Upregulation of Associated Proteins

2008 ◽  
Vol 20 (12) ◽  
pp. 3405-3417 ◽  
Author(s):  
Nameirakpam Dolendro Singh ◽  
Ming Li ◽  
Sueng-Bum Lee ◽  
Danny Schnell ◽  
Henry Daniell
Keyword(s):  
Envelope Membrane ◽  
Associated Proteins ◽  
Inner Envelope
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