scholarly journals ORA47 is a transcriptional regulator of a general stress response hub

2021 ◽  
Author(s):  
Liping Zeng ◽  
Hao Chen ◽  
Yaqi Wang ◽  
Derrick Hicks ◽  
Haiyan Ke ◽  
...  
Keyword(s):  
Stress Response ◽  
Protein S ◽  
Regulatory Proteins ◽  
Adaptive Responses ◽  
Genetic Studies ◽  
General Stress Response ◽  
Calmodulin Binding ◽  
General Stress ◽  
Cellular Events ◽  
Inducible Protein

Transcriptional regulators of general stress response (GSR) reprogram expression of selected genes to transduce informational signals into cellular events, ultimately manifested in plant's ability to cope with environmental challenges. Identification of the core GSR regulatory proteins will uncover the principal modules and their mode of action in the establishment of adaptive responses. To define the GSR regulatory components, we employed a yeast-one-hybrid assay to identify the protein(s) that binds to the previously established functional GSR motif, coined Rapid Stress Response Element (RSRE). This led to the isolation of ORA47 (octadecanoid-responsive AP2/ERF-domain transcription factor 47), a Methyl jasmonate (MeJA) inducible protein. Subsequently, the ORA47 transcriptional activity was confirmed using RSRE-driven Luciferase (LUC) activity assay performed in the ORA47 loss- and gain-of-function lines introgressed into the 4xRSRE::Luc background. In addition, the prime contribution of CALMODULIN-BINDING TRANSCRIPTIONAL ACTIVATOR3 (CAMTA3) protein in induction of RSRE was reaffirmed by genetic studies. Moreover, exogenous application of MeJA led to enhanced levels of ORA47 and CAMTA3 transcripts, and the induction of RSRE::LUC activity. Metabolic analyses illustrated the reciprocal functional inputs of ORA47 and CAMTA3 in increasing JA levels. Lastly, transient assays identified JASMONATE ZIM-domain1 (JAZ1) as a repressor of RSRE::LUC activity.

scholarly journals Mtl1 Is Required to Activate General Stress Response through Tor1 and Ras2 Inhibition under Conditions of Glucose Starvation and Oxidative Stress

2010 ◽  
Vol 285 (25) ◽  
pp. 19521-19531 ◽  
Author(s):  
Mima Ivanova Petkova ◽  
Nuria Pujol-Carrion ◽  
Javier Arroyo ◽  
Jesús García-Cantalejo ◽  
Maria Angeles de la Torre-Ruiz
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Glucose Starvation ◽  
General Stress Response ◽  
General Stress ◽  
And Oxidative Stress

scholarly journals Modeling the functioning of YtvA in the general stress response in Bacillus subtilis

2013 ◽  
Vol 9 (9) ◽  
pp. 2331 ◽  
Author(s):  
Jeroen B. van der Steen ◽  
Yusuke Nakasone ◽  
Johnny Hendriks ◽  
Klaas J. Hellingwerf
Keyword(s):  
Bacillus Subtilis ◽  
Stress Response ◽  
General Stress Response ◽  
General Stress

scholarly journals Development and optimization of an EGFP-based reporter for measuring the general stress response inListeria monocytogenes

Bioengineered ◽  
2012 ◽  
Vol 3 (2) ◽  
pp. 93-103 ◽  
Author(s):  
Marta Utratna ◽  
Eoin Cosgrave ◽  
Claas Baustian ◽  
Rhodri Ceredig ◽  
Conor O’Byrne
Keyword(s):  
Stress Response ◽  
General Stress Response ◽  
General Stress

scholarly journals Complex general stress response regulation in Sphingomonas melonis Fr1 revealed by transcriptional analyses

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Lisa Gottschlich ◽  
Petra Geiser ◽  
Miriam Bortfeld-Miller ◽  
Christopher M. Field ◽  
Julia A. Vorholt
Keyword(s):  
Stress Response ◽  
General Stress Response ◽  
General Stress

Participation of two general stress response proteins from Xanthomonas citri subsp. citri in environmental stress adaptation and virulence

2020 ◽  
Vol 96 (8) ◽  
Author(s):  
María Victoria Barcarolo ◽  
Natalia Gottig ◽  
Jorgelina Ottado ◽  
Betiana S Garavaglia
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Recombinant Proteins ◽  
Citrus Canker ◽  
Freezing Stress ◽  
Protective Effects ◽  
Xanthomonas Citri ◽  
General Stress Response ◽  
General Stress ◽  
And Oxidative Stress

ABSTRACT Xanthomonas citri subsp. citri (Xcc) is the bacteria responsible for citrus canker. During its life cycle Xcc is found on leaves as epiphyte, where desiccation conditions may occur. In this work, two Xcc genes, XAC0100 and XAC4007, predicted in silico to be involved in general stress response, were studied under salt, osmotic, desiccation, oxidative and freezing stress, and during plant-pathogen interaction. Expression of XAC0100 and XAC4007 genes was induced under these stress conditions. Disruption of both genes in Xcc caused decreased bacterial culturability under desiccation, freezing, osmotic and oxidative stress. Importantly, the lack of these genes impaired Xcc epiphytic fitness. Both Xac0100 and Xac4007 recombinant proteins showed protective effects on Xanthomonas cells subjected to drought stress. Also, Escherichia coli overexpressing Xac4007 showed a better performance under standard culture, saline and osmotic stress and were more tolerant to freezing and oxidative stress than wild type E. coli. Moreover, both Xac0100 and Xac4007 recombinant proteins were able to prevent the freeze-thaw-induced inactivation of L-Lactate dehydrogenase. In conclusion, Xac0100 and Xac4007 have a relevant role as bacteria and protein protectors; and these proteins are crucial to bacterial pathogens that must face environmental stressful conditions that compromise the accomplishment of the complete virulence process.

scholarly journals Regulation of the Erythrobacter litoralis DSM 8509 general stress response by visible light

2019 ◽  
Vol 112 (2) ◽  
pp. 442-460 ◽  
Author(s):  
Aretha Fiebig ◽  
Lydia M. Varesio ◽  
Xiomarie Alejandro Navarreto ◽  
Sean Crosson
Keyword(s):  
Stress Response ◽  
Visible Light ◽  
General Stress Response ◽  
General Stress ◽  
Erythrobacter Litoralis
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