scholarly journals Molecular Identification and Characterization of Two Rubber Dandelion Amalgaviruses

2017 ◽  
Author(s):  
Humberto Debat ◽  
Zinan Luo ◽  
Brian J. Iaffaldano ◽  
Xiaofeng Zhuang ◽  
Katrina Cornish
Keyword(s):  
New Species ◽  
Genome Architecture ◽  
Rna Dependent Rna Polymerase ◽  
Dynamic Distribution ◽  
Persistent Viruses ◽  
Rnaseq Data ◽  
Putative Coat Protein ◽  
Two New Species ◽  
Identification And Characterization

AbstractThe Amalgaviridae family comprise persistent viruses that share the genome architecture of Totiviridae and gene evolutionary resemblance to Partitiviridae. Two genera have been assigned to this family, including genus Amalgavirus consisting in nine recognized species, corresponding to plant infecting viruses with dsRNA monosegmented genomes of ca. 3.4 kb. Here, we present the molecular characterization of two novel viruses detected in rubber dandelion (Taraxacum kok-saghyz). The sequenced viruses are 3,409 and 3,413 nt long, including two partially overlapping ORFs encoding a putative coat protein and an RNA-dependent RNA polymerase (RdRP). Phylogenetic insights based on the RdRP suggest them to be members of two new species within the Amalgavirus genus. Multiple independent RNAseq data suggest that the identified viruses have a dynamic distribution and low relative RNA levels in infected plants. Virus presence was not associated with any apparent symptoms on the plant hosts. We propose the names rubber dandelion latent virus 1 & 2 to the detected amalgaviruses; the first viruses to be associated to this emergent and sustainable natural rubber crop.

Taxonomic and pathogenic characterization of Phytopythium species from West Azarbaijan, Iran, and description of two new species

Mycologia ◽  
2021 ◽  
pp. 1-17
Author(s):  
Sevda Rezaei ◽  
Masoud Abrinbana ◽  
Youbert Ghosta
Keyword(s):  
New Species ◽  
Two New Species

Description of two new species of the genus Thaparocleidus Jain, 1952 (Monogenea, Dactylogyridae) from freshwater fish in India: morphological and molecular phylogenetic evidence

2012 ◽  
Vol 87 (2) ◽  
pp. 160-173 ◽  
Author(s):  
A. Chaudhary ◽  
H.S. Singh
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Closely Related Species ◽  
Molecular Biological Analysis ◽  
Wallago Attu ◽  
Two New Species ◽  
Molecular Phylogenetic ◽  
Secondary Structure Models ◽  
Large Subunit Rdna

AbstractThe present paper describes the taxonomy of two new monogeneans, namely, Thaparocleidus longiphallus sp. n. and T. siloniansis sp. n., based on morphological, morphometric and molecular biological analysis, collected from the fish Wallago attu (Bloch & Schn.) and Silonia silondia (Ham.), respectively, at Meerut, UP, India. Genetic characterization of the two new species is based on sequence analyses of the rDNA 28S gene using neighbour-joining and maximum-parsimony techniques. These methods are congruent in depicting T. longiphallus sp. n. and T. siloniansis sp. n. as closely related species, but distinct from each other and forming a subclade with other species of the genus Thaparocleidus Jain, 1952. Secondary-structure models of the large subunit rDNA of the two species were also predicted using a combined comparative and thermodynamic approach. Molecular morphometric and phylogenetic relationships of the isolates of the Thaparocleidus species are discussed in detail.

Notes on some lesser known crickets of the tribe Podoscirtini (Podoscirtinae: Gryllidae: Orthoptera) from India 

Zootaxa ◽  
2020 ◽  
Vol 4821 (1) ◽  
pp. 49-70
Author(s):  
ASHOK KUMAR MEENA ◽  
RAJAMANI SWAMINATHAN ◽  
RAJENDRA NAGAR
Keyword(s):  
New Species ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Tropical Region ◽  
Two New Species

The present paper deals with a description of two new species of crickets of the subfamily Podoscirtinae (Orthoptera: Gryllidae), Trelleora khasiana sp. nov. and Indotrella maculata sp. nov., from India; besides, some additional morphological characteristics of the already reported species, Indotrella angusta Gorochov, 2003 is also presented. The morphological characterization of these two new species is supported with suitable line diagrams and plates. A checklist of crickets of Podoscirtinae for the Asia-tropical region is included.

scholarly journals Description of two new species of Physopyxis and redescription of P. lyra (Siluriformes: Doradidae)

2005 ◽  
Vol 3 (4) ◽  
pp. 625-636 ◽  
Author(s):  
Leandro M. Sousa ◽  
Lúcia H. Rapp Py-Daniel
Keyword(s):  
New Species ◽  
Geographic Distribution ◽  
Type Species ◽  
Coracoid Process ◽  
Two New Species ◽  
Precise Characterization ◽  
Lateral Plates

The genus and species Physopyxis lyra was originally described based on only one very distinctive specimen. Analysis of a larger number of specimens provided a more precise characterization of the genus and the type species. Additionally, two new species are described based on material already available in museums and new collections. Physopyxis lyra is characterized by having a well developed coracoid process with divergent tips, and only one series of spines on the lateral plates. Physopyxis ananas, new species, has a thin coracoid process, convergent, and more than one series of spines on the lateral plates. Physopyxis cristata, new species, is a slender species, characterized by an incomplete series of lateral plates and the presence of a middorsal series of spines formed by the tips of the vertebral neural spines. The poorly known geographic distribution of the genus Physopyxis is vastly enlarged to include various drainages of the Amazon and Essequibo basins.

Identification and characterization of Zika virus NS5 RNA-dependent RNA polymerase inhibitors

2019 ◽  
Vol 54 (4) ◽  
pp. 502-506 ◽  
Author(s):  
Yuan Lin ◽  
Hongjuan Zhang ◽  
Weibao Song ◽  
Shuyi Si ◽  
Yanxing Han ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Zika Virus ◽  
Rna Dependent Rna Polymerase ◽  
Polymerase Inhibitors ◽  
Identification And Characterization

scholarly journals Isolation and Characterization of Mycoplasma columbinum and Mycoplasma columborale, Two New Species from Pigeons

1978 ◽  
Vol 28 (4) ◽  
pp. 538-546 ◽  
Author(s):  
T. SHIMIZU ◽  
H. ERNO ◽  
H. NAGATOMO
Keyword(s):  
New Species ◽  
Isolation And Characterization ◽  
Two New Species

Morphological and phylogenetic characterization of novel Metarhizium species in Guizhou, China

Phytotaxa ◽  
2019 ◽  
Vol 419 (2) ◽  
pp. 189-196
Author(s):  
WAN-HAO CHEN ◽  
YAN-FENG HAN ◽  
JIAN-DONG LIANG ◽  
ZONG-QI LIANG
Keyword(s):  
New Species ◽  
Morphological Characteristics ◽  
Phylogenetic Characterization ◽  
Two New Species

Two new species, Metarhizium lepidopterorum and Metarhizium rongjiangense, which are both parasitic on lepidopteran pupae, are reported. Both the morphological characteristics and DNA-based phylogenies from a multigene dataset-based analysis supported the identification of two new species. Both species can be distinguished from other species of Metarhizium by their lecanicillium-like conidiophores, longer phialides and the presence of only one type of conidium, which is smaller and fusiform in M. lepidopterorum and smaller and of subwedge shape in M. rongjiangense. The combined dataset (ITS, RPB1, RPB2 and TEF) was analysed phylogenetically in Metarhizium spp. The new species described herein are clearly distinct from other Metarhizium species.

scholarly journals Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1062 ◽  
Author(s):  
Maela León ◽  
Mónica Berbegal ◽  
José M. Rodríguez-Reina ◽  
Georgina Elena ◽  
Paloma Abad-Campos ◽  
...  
Keyword(s):  
New Species ◽  
Sequence Analysis ◽  
Phylogenetic Species ◽  
Shoot Blight ◽  
Pathogenicity Tests ◽  
Almond Orchards ◽  
One Year ◽  
Prevalent Species ◽  
Identification And Characterization

Two hundred and twenty-five Diaporthe isolates were collected from 2005 to 2019 in almond orchards showing twig cankers and shoot blight symptoms in five different regions across Spain. Multilocus DNA sequence analysis with five loci (ITS, tub, tef-1α, cal and his), allowed the identification of four known Diaporthe species, namely: D. amygdali, D. eres, D. foeniculina and D. phaseolorum. Moreover, a novel phylogenetic species, D. mediterranea, was described. Diaporthe amygdali was the most prevalent species, due to the largest number of isolates (85.3%) obtained from all sampled regions. The second most frequent species was D. foeniculina (10.2%), followed by D. mediterranea (3.6%), D. eres and D. phaseolorum, each with only one isolate. Pathogenicity tests were performed using one-year-old almond twigs cv. Vayro and representative isolates of the different species. Except for D. foeniculina and D. phaseolorum, all Diaporthe species were able to cause lesions significantly different from those developed on the uninoculated controls. Diaporthe mediterranea caused the most severe symptoms. These results confirm D. amygdali as a key pathogen of almonds in Spain. Moreover, the new species, D. mediterranea, should also be considered as a potential important causal agent of twig cankers and shoot blight on this crop.

Morphological and molecular characterization of two new species of the genus Aporcelinus Andrássy, 2009 (Nematoda, Dorylaimida, Aporcelaimidae) from the USA, with new insights on the phylogeny of the genus

2018 ◽  
Vol 94 ◽  
Author(s):  
S. Álvarez-Ortega ◽  
S.A. Subbotin ◽  
R. Peña-Santiago
Keyword(s):  
New Species ◽  
Ventral Side ◽  
28S Rrna ◽  
Neck Length ◽  
Female Tail ◽  
Body Diameter ◽  
Two New Species ◽  
Close Relationship ◽  
The Usa

Abstract Two new species of the genus Aporcelinus from the USA are described and illustrated. Aporcelinus floridensis sp. n. is characterized by its 1.12–1.52 mm long body, lip region offset by marked constriction and 14.5–17.0 μm broad with perioral liplets, odontostyle 16.5–20.0 μm at its ventral side and 1.1–1.2 times the lip region diameter, neck 316–395 μm long, pharyngeal expansion occupying 43–48% of total neck length, uterus simple and 33–56 μm long or 0.8–1.2 times the corresponding body diameter, V = 48–54, female tail conical (36–49 μm long, c = 27–41, c’ = 1.2–2.0) with finely rounded terminus and no hyaline portion, and male absent. Aporcelinus paolae sp. n. is characterized by its 1.29–1.80 mm long body, lip region offset by marked constriction and 14–16 μm broad, odontostyle 15–17 μm at its ventral side and 1.0–1.1 times the lip region diameter, neck 314–397 μm long, pharyngeal expansion occupying 43–53% of total neck length, uterus tripartite and 128–164 μm long or 2.6–3.6 times the corresponding body diameter, V = 53–57, female tail conical (30–39 μm long, c = 40–51, c’ = 1.1–1.3) with finely rounded terminus and variably re-curved dorsad, male tail conical (27–36 μm, c = 39–59, c’ = 0.9–1.2), ventrally straight and dorsally convex, spicules 48–54 μm long, and 7–9 irregularly spaced ventromedian supplements lacking hiatus. The analyses of the D2-D3 expansion segments of 28S rRNA (LSU) gene sequences of the two new species confirmed the monophyly of the genus, based upon currently available data, showing a close relationship between the genera Aporcelinus and Makatinus, and justified the placement of Aporcelaimellus, Makatinus and Aporcelinus under the subfamily Aporcelaimellinae.

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