Instantaneous isotropic volumetric imaging of fast biological processes
Keyword(s):
AbstractCapturing highly dynamic biological processes at sub-cellular resolution is a recurring challenge in biology. Here we show that combining selective volume illumination with simultaneous acquisition of orthogonal light-fields yields 3D images with high, isotropic spatial resolution and free of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate Medaka heart and blood flow imaging with single-cell resolution and free of motion artefacts at volume rates up to 200Hz.
2020 ◽
Vol 59
(SK)
◽
pp. SKKE16
◽
Keyword(s):
Keyword(s):
Keyword(s):
1998 ◽
Vol 24
(2)
◽
pp. 203-214
◽
Keyword(s):