scholarly journals Respiratory supercomplexes provide metabolic efficiency in zebrafish

2019 ◽  
Author(s):  
Carolina García-Poyatos ◽  
Sara Cogliati ◽  
Enrique Calvo ◽  
Pablo Hernansanz-Agustín ◽  
Sylviane Lagarrigue ◽  
...  
Keyword(s):  
Null Allele ◽  
Physiological Role ◽  
Complex Iii ◽  
Metabolic Efficiency ◽  
Metabolic Gene Expression ◽  
Oxphos System ◽  
Assembly Factor ◽  
Quaternary Structures ◽  
Expression Program

The oxidative phosphorylation (OXPHOS) system is a dynamic system in which the respiratory complexes coexist with super-assembled quaternary structures called supercomplexes (SCs). The physiological role of SCs is still disputed. Here we used zebrafish to study the relevance of respiratory SCs. We combined immunodetection analysis and deep data-independent proteomics to characterize these structures and found similar SCs to those described in mice, as well as novel SCs including III2+IV2, I+IV and I+III2+IV2. To study the physiological role of SCs, we generated two null allele zebrafish lines for supercomplex assembly factor 1 (SCAF1). SCAF1-/- fish displayed altered OXPHOS activity due to the disrupted interaction of complex III and IV. SCAF1-/- fish were smaller in size, and showed abnormal fat deposition and decreased female fertility. These physiological phenotypes were rescued by doubling the food supply, which correlated with improved bioenergetics and alterations in the metabolic gene expression program. These results reveal that SC assembly by SCAF1 modulates OXPHOS efficiency and allows for the optimization of metabolic resources.

scholarly journals The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Baisakhi Mondal ◽  
Hongjian Jin ◽  
Satish Kallappagoudar ◽  
Yurii Sedkov ◽  
Tanner Martinez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Neurite Outgrowth ◽  
Histone Deacetylase ◽  
Physiological Role ◽  
Gene Expression Program ◽  
Neural Genes ◽  
Important Regulator ◽  
Neurite Development ◽  
Expression Program

The mitotic deacetylase complex (MiDAC) is a recently identified histone deacetylase (HDAC) complex. While other HDAC complexes have been implicated in neurogenesis, the physiological role of MiDAC remains unknown. Here, we show that MiDAC constitutes an important regulator of neural differentiation. We demonstrate that MiDAC functions as a modulator of a neurodevelopmental gene expression program and binds to important regulators of neurite outgrowth. MiDAC upregulates gene expression of pro-neural genes such as those encoding the secreted ligands SLIT3 and NETRIN1 (NTN1) by a mechanism suggestive of H4K20ac removal on promoters and enhancers. Conversely, MiDAC inhibits gene expression by reducing H3K27ac on promoter-proximal and -distal elements of negative regulators of neurogenesis. Furthermore, loss of MiDAC results in neurite outgrowth defects that can be rescued by supplementation with SLIT3 and/or NTN1. These findings indicate a crucial role for MiDAC in regulating the ligands of the SLIT3 and NTN1 signaling axes to ensure the proper integrity of neurite development.

scholarly journals Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Qpool

2020 ◽  
Vol 6 (26) ◽  
pp. eaba7509 ◽  
Author(s):  
Enrique Calvo ◽  
Sara Cogliati ◽  
Pablo Hernansanz-Agustín ◽  
Marta Loureiro-López ◽  
Adela Guarás ◽  
...  
Keyword(s):  
Quantitative Data ◽  
Functional Role ◽  
Physiological Role ◽  
Oxidative Capacity ◽  
Oxygen Species ◽  
Wild Type ◽  
Assembly Factor ◽  
Native Gel ◽  
Blue Native

Mitochondrial respiratory complexes assemble into supercomplexes (SC). Q-respirasome (III2 + IV) requires the supercomplex assembly factor (SCAF1) protein. The role of this factor in the N-respirasome (I + III2 + IV) and the physiological role of SCs are controversial. Here, we study C57BL/6J mice harboring nonfunctional SCAF1, the full knockout for SCAF1, or the wild-type version of the protein and found that exercise performance is SCAF1 dependent. By combining quantitative data–independent proteomics, 2D Blue native gel electrophoresis, and functional analysis of enriched respirasome fractions, we show that SCAF1 confers structural attachment between III2 and IV within the N-respirasome, increases NADH-dependent respiration, and reduces reactive oxygen species (ROS). Furthermore, the expression of AOX in cells and mice confirms that CI-CIII superassembly segments the CoQ in two pools and modulates CI-NADH oxidative capacity.

Enteral enhancement of glucose disposition by both insulin-dependent and insulin-independent processes. A physiological role of glucagon-like peptide I

Diabetes ◽  
1995 ◽  
Vol 44 (12) ◽  
pp. 1433-1437 ◽  
Author(s):  
D. A. D'Alessio ◽  
R. L. Prigeon ◽  
J. W. Ensinck
Keyword(s):  
Physiological Role ◽  
Insulin Dependent ◽  
Glucagon Like Peptide

Physiological Role of Cyclic Electron Flow in Higher Plants

2012 ◽  
Vol 30 (1) ◽  
pp. 100
Author(s):  
Wei HUANG ◽  
Shi-Bao ZHANG ◽  
Kun-Fang CAO
Keyword(s):  
Higher Plants ◽  
Electron Flow ◽  
Physiological Role ◽  
Cyclic Electron Flow

The Renal Outer Medullary Potassium Channel (ROMK): An Intriguing Pharmacological Target for an Innovative Class of Diuretic Drugs

2018 ◽  
Vol 25 (23) ◽  
pp. 2627-2636 ◽  
Author(s):  
Vincenzo Calderone ◽  
Alma Martelli ◽  
Eugenia Piragine ◽  
Valentina Citi ◽  
Lara Testai ◽  
...  
Keyword(s):  
Physiological Role ◽  
Clinical Use ◽  
Diuretic Activity ◽  
First Line ◽  
Potassium Homeostasis ◽  
Diuretic Drugs ◽  
Pharmacological Target ◽  
Diuretic Agents ◽  
Effective Drugs

In the last four decades, the several classes of diuretics, currently available for clinical use, have been the first line option for the therapy of widespread cardiovascular and non-cardiovascular diseases. Diuretic drugs generally exhibit an overall favourable risk/benefit balance. However, they are not devoid of side effects. In particular, all the classes of diuretics cause alteration of potassium homeostasis. <p> In recent years, understanding of the physiological role of the renal outer medullary potassium (ROMK) channels, has shown an intriguing pharmacological target for developing an innovative class of diuretic agents: the ROMK inhibitors. This novel class is expected to promote diuretic activity comparable to (or even higher than) that provided by the most effective drugs used in clinics (such as furosemide), with limited effects on potassium homeostasis. <p> In this review, the physio-pharmacological roles of ROMK channels in the renal function are reported, along with the most representative molecules which have been currently developed as ROMK inhibitors.

scholarly journals Physiological role of endogenous NO in the chicken proventriculus

1999 ◽  
Vol 79 ◽  
pp. 199
Author(s):  
Takio Kitazawa ◽  
Tetsuro Taneike
Keyword(s):  
Physiological Role ◽  
Chicken Proventriculus

scholarly journals Membrane-surfactant interactions. The role of surfactant in mitochondrial complex III-phospholipid-Triton X-100 mixed micelles.

1986 ◽  
Vol 261 (14) ◽  
pp. 6578-6584
Author(s):  
J M Valpuesta ◽  
J L Arrondo ◽  
M C Barbero ◽  
M Pons ◽  
F M Goñi
Keyword(s):  
Mixed Micelles ◽  
Complex Iii ◽  
Mitochondrial Complex ◽  
Surfactant Interactions ◽  
Triton X

scholarly journals Caspase-14—From Biomolecular Basics to Clinical Approach. A Review of Available Data

2021 ◽  
Vol 22 (11) ◽  
pp. 5575
Author(s):  
Agnieszka Markiewicz ◽  
Dawid Sigorski ◽  
Mateusz Markiewicz ◽  
Agnieszka Owczarczyk-Saczonek ◽  
Waldemar Placek
Keyword(s):  
Cell Death ◽  
Physiological Role ◽  
Skin Barrier ◽  
Clinical Aspects ◽  
Clinical Approach ◽  
Search Term ◽  
Caspase Family ◽  
Skin Conditions ◽  
Theoretical Science

Caspase-14 is a unique member of the caspase family—a family of molecules participating in apoptosis. However, it does not affect this process but regulates another form of programmed cell death—cornification, which is characteristic of the epidermis. Therefore, it plays a crucial role in the formation of the skin barrier. The cell death cycle has been a subject of interest for researchers for decades, so a lot of research has been done to expand the understanding of caspase-14, its role in cell homeostasis and processes affecting its expression and activation. Conversely, it is also an interesting target for clinical researchers searching for its role in the physiology of healthy individuals and its pathophysiology in particular diseases. A summary was done in 2008 by Denecker et al., concentrating mostly on the biotechnological aspects of the molecule and its physiological role. However, a lot of new data have been reported, and some more practical and clinical research has been conducted since then. The majority of studies tackled the issue of clinical data presenting the role of caspase in the etiopathology of many diseases such as retinal dysfunctions, multiple malignancies, and skin conditions. This review summarizes the available knowledge on the molecular and, more interestingly, the clinical aspects of caspase-14. It also presents how theoretical science may pave the way for medical research. Methods: The authors analyzed publications available on PubMed until 21 March 2021, using the search term “caspase 14”.

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