scholarly journals Development of SSR markers related to seed storability traits in maize subjected to artificial seed aging conditions

2019 ◽  
Author(s):  
Xiaoyang Guo ◽  
Chengxu Gong ◽  
Shan Liu ◽  
Chenchen Feng ◽  
Xiao Han ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Germination Rate ◽  
Aging Treatment ◽  
Inbred Lines ◽  
Seed Aging ◽  
Maize Germplasm ◽  
Chi Squared ◽  
Aging Conditions ◽  
Combined Selection ◽  
Seed Storability

AbstractSeed storability is an important and complex agronomic trait in maize because annual seed production considerably exceeds consumption. The viability of seeds decreases over time, even when stored at low temperature, until seeds finally lose viability. In our previous study, two inbred lines with significantly different storability, Dong156 with high storage tolerance and Dong237 with low storage tolerance, were selected over six years using a natural seed aging test. In the present study, an F2:3 population and a RIL (recombinant inbred line) population were constructed from these two inbred lines and used to map QTL (quantitative trait loci) with SSR (simple sequence repeat) markers. A phenotypic index of traits related to seed storability that includes germination rate, germination potential, a germination index, a vigor index, seedling weight, and seedling length was generated using the results of an artificial aging treatment. Two consistent regions, cQTL-7 on chromosome 7 and cQTL-10 on chromosome 10, were identified by comparing QTL analysis results from these two populations. After genotyping SSR markers in these two regions, cQTL-7 was remapped to between umc1671 and phi328175 in a 7.97-Mb region, and cQTL-10 was remapped to between umc1648 and phi050 in a 39.15-Mb region. Four SSR markers linked to cQTL-7 and cQTL-10, including umc1671, phi328175, umc1648, and phi050, were identified using a Chi-squared test. The combined selection efficiency of these four markers was 83.94% in 85 RIL lines with high storability, and marker umc1648 exhibited the highest efficiency value of 88.89%. These results indicated that the four SSR markers developed in this study could be used for selection of maize germplasm with high seed storability.

scholarly journals Phenotypical Variability of “TURDA” Maize Germplasm for Grain Chemical Composition

1970 ◽  
Vol 66 (1) ◽  
Author(s):  
Voichita HAS ◽  
Ioan HAS ◽  
Doru PAMFIL ◽  
Ana COPANDEAN ◽  
Sorin CAMPEAN
Keyword(s):  
Grain Quality ◽  
Selection Criterion ◽  
Quality Attributes ◽  
Inbred Lines ◽  
Phenotypic Correlation ◽  
Starch Concentration ◽  
Maize Germplasm ◽  
Grain Starch ◽  
Food And Feed ◽  
Phenotypical Variability

Maize grain has many and diverse uses in the food and feed industry. The diversity of applications requires characteristics of quality in accordance to that. To examine phenotypical diversity in the grain content, it was evaluated a total of 754 maize samples: 265 local populations (landraces); 59 synthetics/composites; 430 “TURDA” inbred lines for their grain quality attributes. Comparison of the inbred lines diversity is on average the most divergent in grain starch concentration (range value 19.9) from landraces (range value 11.8) and synthetics (range value 12.5). The grain oil and ash content showed high variability among the genotypes. The quality attributes in most of the cases showed positive phenotypic correlation except grain starch contents which was negatively correlated at phenotypic levels. The objective of this study was to evaluate the potential of maize “TURDA” germplasm in according to its grain quality content, such as: protein, oil, fiber, ash and starch concentration; to estimate the extent of phenotypical variability and correlation for various quality components to formulate a selection criterion in a breeding program.

scholarly journals Comparative analysis of genetic diversity among the maize inbred lines (Zea mays L.) obtained by RAPD and SSR markers

2008 ◽  
Vol 51 (1) ◽  
pp. 183-192 ◽  
Author(s):  
Silvia Graciele Hülse de Souza ◽  
Valéria Carpentieri-Pípolo ◽  
Claudete de Fátima Ruas ◽  
Valdemar de Paula Carvalho ◽  
Paulo Maurício Ruas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Zea Mays L ◽  
Inbred Lines ◽  
Pedigree Data ◽  
Dice Coefficient ◽  
Quantitative Characters ◽  
Maize Inbred Lines ◽  
Ssr Primers ◽  
Selection Of

The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinate’s analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize the differences between the quantitative characters. Because they assess the distinct regions of the genome, the selection of one or other marker would depend on the characteristics of the material used and the objectives of the project.

scholarly journals Analysis of Population Structure and Association Mapping among Popcorn Inbred Lines using SSR markers

2013 ◽  
Vol 45 (4) ◽  
pp. 358-368 ◽  
Author(s):  
Jin-Sun Jang ◽  
Eun-Ha Chang ◽  
Kyu Jin Sa ◽  
Byeong Wan Kim ◽  
Jong-Hwa Kim ◽  
...  
Keyword(s):  
Population Structure ◽  
Association Mapping ◽  
Ssr Markers ◽  
Inbred Lines

Genetic Diversity among CIMMYT Maize Inbred Lines Investigated with SSR Markers

Crop Science ◽  
2004 ◽  
Vol 44 (6) ◽  
pp. 2230-2237 ◽  
Author(s):  
X. C. Xia ◽  
J. C. Reif ◽  
D. A. Hoisington ◽  
A. E. Melchinger ◽  
M. Frisch ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Inbred Lines ◽  
Maize Inbred Lines

Analysis of genetic diversity and relationships among waxy maize inbred lines in Korea using SSR markers

2010 ◽  
Vol 32 (4) ◽  
pp. 375-384 ◽  
Author(s):  
Kyu Jin Sa ◽  
Jong Yeol Park ◽  
Ki Jin Park ◽  
Ju Kyong Lee
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Inbred Lines ◽  
Waxy Maize ◽  
Maize Inbred Lines

scholarly journals Genetic Diversity in CIMMYT Nontemperate Maize Germplasm: Landraces, Open Pollinated Varieties, and Inbred Lines

Crop Science ◽  
2008 ◽  
Vol 48 (2) ◽  
pp. 617-624 ◽  
Author(s):  
M. L. Warburton ◽  
J. C. Reif ◽  
M. Frisch ◽  
M. Bohn ◽  
C. Bedoya ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Inbred Lines ◽  
Maize Germplasm

scholarly journals Effect of Natural Seed Aging on Root and Shoot Traits in Bread Wheat (Triticum aestivum L.) Cultivars

2021 ◽  
Vol 9 (4) ◽  
pp. 759-763
Author(s):  
Hayati Akman
Keyword(s):  
Bread Wheat ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Seed Storage ◽  
Wheat Cultivars ◽  
Coleoptile Length ◽  
Seed Aging ◽  
Long Term Storage ◽  
Term Storage

This study targeted to elucidate the effect of seed aging on germination and emergence rates with and shoot characteristics in wheat cultivars. For this purpose, different bread wheat cultivars stored for 7 years and non-stored were compared for coleoptile length, root mass, shoot mass, root length as well as germination and seedling emergence rates. Here, the evidence suggested that seed storage over a prolonged period affected root and Shoot growth, coleoptile length, seed germination, and seedling emergence rates adversely. By linking germination and emergence rates, the data presented here indicated that a reduction in emergence rate in long-term storage was higher than that in the germination rate. It was also found that there were significant variations among the wheat cultivars about investigated traits during long-term storage. However, the emergence rates of Kate A1 and Flamura 85 were not affected substantially by long-term storage. The study suggested future studies to focus on clarification of the process controlling natural seed aging as such knowledge allows clue the eventual consequences of long-term storage.

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