scholarly journals In Vivo Transfection of Naked DNA into Xenopus Tadpole Tail Muscle

2017 ◽  
Vol 2017 (11) ◽  
pp. pdb.prot099366 ◽  
Author(s):  
Lindsey Marshall ◽  
Fabrice Girardot ◽  
Barbara A. Demeneix ◽  
Laurent Coen
Keyword(s):  
Xenopus Tadpole ◽  
Naked Dna ◽  
Tadpole Tail ◽  
In Vivo Transfection ◽  
Tail Muscle

scholarly journals Corticotropin-Releasing Factor Is Cytoprotective in Xenopus Tadpole Tail: Coordination of Ligand, Receptor, and Binding Protein in Tail Muscle Cell Survival

Endocrinology ◽  
2006 ◽  
Vol 147 (3) ◽  
pp. 1498-1507 ◽  
Author(s):  
Graham C. Boorse ◽  
Cyrus A. Kholdani ◽  
Audrey F. Seasholtz ◽  
Robert J. Denver
Keyword(s):  
Binding Protein ◽  
Explant Culture ◽  
Corticotropin Releasing Factor ◽  
Environmental Stressors ◽  
Crf1 Receptor ◽  
Tadpole Tail ◽  
Locomotory Organ ◽  
Urocortin 1 ◽  
Tail Muscle

Upon metamorphosis, amphibian tadpoles lose their tails through programmed cell death induced by thyroid hormone (T3). Before transformation, the tail functions as an essential locomotory organ. The binding protein for the stress neuropeptide corticotropin-releasing factor (CRF; CRF-BP) is strongly up-regulated in the tail of Xenopus tadpoles during spontaneous or T3-induced metamorphosis. This finding led us to investigate physiological roles for CRF and CRF-BP in tadpole tail. We found CRF, CRF-BP, and functional CRF1 receptor in tail and CRF and functional CRF1 receptors, but not CRF-BP, in the tail muscle-derived cell line XLT-15. CRF, acting via the CRF1 receptor, slowed spontaneous tail regression in explant culture and caused a reduction in caspase 3/7 activity. CRF increased, but stable CRF-BP overexpression decreased, [3H]thymidine incorporation in XLT-15 cells. Overexpression of CRF-BP in vivo accelerated the loss of tail muscle cells during spontaneous metamorphosis. Lastly, exposure of tail explants to hypoxia increased CRF and urocortin 1 but strongly decreased CRF-BP mRNA expression. We show that CRF is expressed in tadpole tail, is up-regulated by environmental stressors, and is cytoprotective. The inhibitory binding protein for CRF is regulated by hormones or by environmental stressors and can modulate CRF bioactivity.

Thyroid hormone regulation of translation in tadpole tail muscle

1980 ◽  
Vol 58 (6) ◽  
pp. 461-468 ◽  
Author(s):  
Mohammed Saleem ◽  
Burr G. Atkinson
Keyword(s):  
Protein Synthesis ◽  
Synthetic Activity ◽  
Translational Efficiency ◽  
Hormone Regulation ◽  
Tadpole Tail ◽  
Inhibitor Of Protein Synthesis ◽  
Tail Muscle ◽  
Translational Level

Recent in vivo and in vitro studies with polyribosomes from the tail muscle of T3-treated tadpoles establish that this hormone initiates a regulating effect on tadpole tail muscle which operates at the translational level and results in an overall decreased rate of protein synthesis (Saleem, M. &Atkinson, B. G. (1978) J. Biol. Chem. 253, 1378–1384). This hormone-induced decrease in the rate of protein synthesis is partially, if not wholly, due to the presence of a sarcoplasmic factor(s) inhibiting ribosomal translational efficiency. This research employs the use of a reconstituted, cell-free polypeptide synthesizing system as a means to substantiate the presence of an inhibitor and further elucidate the mechanism by which this inhibitory factor(s) depresses protein synthesis. The results of this study further demonstrate the presence of an inhibitor of protein synthesis in the tail muscle sarcoplasm of T3-treated tadpoles and suggest that this depressed synthetic activity results from an interaction of the inhibitor with ribosomal or polyribosomal constituents.

1004: In-Vivo Transfection of the Rat Corpus Cavernosum Using a Non-Viral, Linear Polyethylenimine Gene Delivery System: A Novel Application

2006 ◽  
Vol 175 (4S) ◽  
pp. 323-324 ◽  
Author(s):  
Joseph Dall'era ◽  
Sweaty Koul ◽  
Jesse Mills ◽  
Jeremy Myers ◽  
Randall B. Meacham ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Corpus Cavernosum ◽  
Gene Delivery System ◽  
System A ◽  
In Vivo Transfection ◽  
Linear Polyethylenimine

Non-Viral Vectors for Gene Delivery

2018 ◽  
Vol 9 (1) ◽  
pp. 4-11 ◽  
Author(s):  
Aparna Bansal ◽  
Himanshu
Keyword(s):  
Gene Therapy ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Expression System ◽  
Target Cells ◽  
Specific Expression ◽  
Naked Dna

Introduction: Gene therapy has emerged out as a promising therapeutic pave for the treatment of genetic and acquired diseases. Gene transfection into target cells using naked DNA is a simple and safe approach which has been further improved by combining vectors or gene carriers. Both viral and non-viral approaches have achieved a milestone to establish this technique, but non-viral approaches have attained a significant attention because of their favourable properties like less immunotoxicity and biosafety, easy to produce with versatile surface modifications, etc. Literature is rich in evidences which revealed that undoubtedly, non–viral vectors have acquired a unique place in gene therapy but still there are number of challenges which are to be overcome to increase their effectiveness and prove them ideal gene vectors. Conclusion: To date, tissue specific expression, long lasting gene expression system, enhanced gene transfection efficiency has been achieved with improvement in delivery methods using non-viral vectors. This review mainly summarizes the various physical and chemical methods for gene transfer in vitro and in vivo.

Accumulation of Human Apolipoprotein-E in Rat Plasma After in vivo Intramuscular Injection of Naked DNA

1994 ◽  
Vol 200 (1) ◽  
pp. 298-305 ◽  
Author(s):  
V.M. Fazio ◽  
S. Fazio ◽  
M. Rinaldi ◽  
M.V. Catani ◽  
S. Zotti ◽  
...  
Keyword(s):  
Apolipoprotein E ◽  
Intramuscular Injection ◽  
Rat Plasma ◽  
Human Apolipoprotein ◽  
Naked Dna

scholarly journals Long-Distance Signals Are Required for Morphogenesis of the Regenerating Xenopus Tadpole Tail, as Shown by Femtosecond-Laser Ablation

PLoS ONE ◽  
2011 ◽  
Vol 6 (9) ◽  
pp. e24953 ◽  
Author(s):  
Jessica P. Mondia ◽  
Michael Levin ◽  
Fiorenzo G. Omenetto ◽  
Ryan D. Orendorff ◽  
Mary Rose Branch ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Femtosecond Laser ◽  
Femtosecond Laser Ablation ◽  
Long Distance ◽  
Xenopus Tadpole ◽  
Tadpole Tail

In vivo monitoring of DNA vaccine gene expression using firefly luciferase as a naked DNA

Vaccine ◽  
2006 ◽  
Vol 24 (16) ◽  
pp. 3057-3062 ◽  
Author(s):  
Y JEON ◽  
Y CHOI ◽  
J KANG ◽  
J CHUNG ◽  
Y LEE ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Vaccine ◽  
Firefly Luciferase ◽  
In Vivo Monitoring ◽  
Naked Dna ◽  
Vaccine Gene

Prevention of hypoxic liver injury by in vivo transfection of the human bcl-2 gene

1997 ◽  
Vol 29 (1-2) ◽  
pp. 384-385 ◽  
Author(s):  
K. Yamabe] ◽  
W. Kamiike ◽  
S. Shimizu ◽  
S. Waguri ◽  
J. Hasegawa ◽  
...  
Keyword(s):  
Liver Injury ◽  
In Vivo Transfection ◽  
Hypoxic Liver Injury

scholarly journals THE EFFECT OF COLCHICINE ON MYOGENESIS IN VIVO IN RANA PIPIENS AND RHODNIUS PROLIXUS (HEMIPTERA)

1968 ◽  
Vol 39 (3) ◽  
pp. 544-555 ◽  
Author(s):  
Robert H. Warren
Keyword(s):  
Cell Shape ◽  
Rana Pipiens ◽  
Colchicine Treatment ◽  
Rhodnius Prolixus ◽  
Microtubule Disruption ◽  
Blood Sucking ◽  
Tadpole Tail ◽  
Differentiation And Dedifferentiation ◽  
Regeneration Blastema

The effect of colchicine on myogenesis in vivo has been studied in the regenerating tadpole tail of the frog, Rana pipiens, and in the abdominal molting muscles of a blood-sucking bug, Rhodnius prolixus Stål. Colchicine is shown to disrupt microtubules in the differentiating muscle cells of both these organisms. The disruption of microtubules is correlated with a loss of longitudinal anisometry in the myoblasts and myotubes of the regeneration blastema in the tadpole tail. Before colchicine treatment, the myotubes contain longitudinally oriented myofibrils. After colchicine treatment, rounded, multinucleate myosacs containing randomly oriented myofibrils are present. It is suggested that the primary function of microtubules in myogenesis in the Rana pipiens tadpole is the maintenance of cell shape. The abdominal molting muscles of Rhodnius undergo repeated phases of differentiation and dedifferentiation of the sarcoplasm. However, the longitudinal anisometry of the muscle fibers is maintained in all phases by the attachments of the ends of the fibers to the exoskeleton, and microtubule disruption does not alter cell shape. The orientation of the developing myofibrils is also unaltered, indicating that the microtubules do not directly align or support the myofibrils in this system.

scholarly journals Control of muscle regeneration in the Xenopus tadpole tail byPax7

Development ◽  
2006 ◽  
Vol 133 (12) ◽  
pp. 2303-2313 ◽  
Author(s):  
Y. Chen ◽  
G. Lin ◽  
J. M. W. Slack
Keyword(s):  
Muscle Regeneration ◽  
Xenopus Tadpole ◽  
Tadpole Tail
Sign in / Sign up

Export Citation Format

Share Document