Cloning, purification, crystallization and preliminary X-ray studies of the putative type VI secretion immunity protein Tli5 (PA5088) fromPseudomonas aeruginosa
2014 ◽
Vol 70
(7)
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pp. 903-905
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Keyword(s):
X Ray
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The putative protein PA5089 fromPseudomonas aeruginosahas recently been identified as a Tle5 phospholipase effector from a type VI secretion system (T6SS), and its toxicity can be neutralized by the cognate immunity protein Tli5 (PA5088). Here, the expression, purification, crystallization and preliminary crystallographic analysis of PA5088 are reported. X-ray diffraction data were collected from selenomethionine-derivatized PA5088 crystals to a resolution of 2.55 Å. The crystals belonged to space groupP21, with unit-cell parametersa= 64.002,b= 104.744,c= 90.168 Å.
2013 ◽
Vol 69
(4)
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pp. 421-424
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2014 ◽
Vol 70
(9)
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pp. 1224-1227
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2014 ◽
Vol 70
(10)
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pp. 1372-1375
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2015 ◽
Vol 71
(1)
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pp. 54-56
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2014 ◽
Vol 70
(6)
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pp. 738-741
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