The protective effect of olive cake treatment on oxidant/antioxidant biomarkers, on serum, red blood cells and liver, in streptozotocin-induced diabetic rats fed cholesterol-enriched diet

2019 ◽  
Vol 50 (4) ◽  
pp. 785-798
Author(s):  
Yahiaoui Zidan ◽  
Sherazede Bouderbala ◽  
Cherrad Hayet ◽  
Bouchenak Malika
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Diabetic Rats ◽  
Male Rats ◽  
Olive Cake ◽  
Content Type ◽  
Enzymes Activities ◽  
Antioxidant Enzymes Activities

Purpose The purpose of this study is to determine the effect of olive cake (OC) on lipid peroxidation as well as antioxidant enzymes activities of serum, red blood cells (RBCs) and liver, in streptozotocin (STZ)-induced-diabetic rat fed cholesterol-enriched diet. Design/methodology/approach Hypercholesterolemic male rats were rendered diabetic (HC-D) by a single intraperitoneal injection dose of STZ (35 mg/kg BW). HC-D rats were divided into two groups fed for 28d a diet supplemented with OC at 7.5 percent (HC-D-OC) or not (HC-D). A control group (C) was submitted to standard diet containing 20 per cent casein for the same experimental period. Findings RBCs, serum and liver thiobarbituric acid reactive substances (TBARS) contents were significantly increased in HC-D, compared to C group (p = 0.04, p = 0.02 and 0.03). These values were significantly decreased (48 per cent and 64 per cent; p = 0.02 and p = 0.0007) in serum and liver of HC-D-OC vs HC-D group. In RBCs, superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activities were, respectively, 1.5, 2- and 1.7-fold higher (p = 0.03, p = 0.008 and p = 0.03) in HC-D group compared to HC group. In serum and liver, SOD, CAT and GST activities were, respectively, 1.3-, 2.6- and 1.6-fold increased (p = 0.03, p = 0.007 and p = 0.02). In HC-D-OC compared to HC-D group, RBCs glutathione peroxidase (GSH-Px), CAT and GST activities were, respectively, 2.1-, 3.3- and 2.1-fold higher (p = 0.04, p = 0.0009 and p = 0.03). In serum, SOD and CAT activities were, respectively, 1.5- and 1.9-fold increased (p = 0.02, p = 0.02). In liver, SOD, GSH-PX, CAT and GST activities were significantly increased (p = 0.005, p = 0.03, p = 0.02 and p = 0.04). Originality/value In diabetic rats-fed cholesterol-enriched diet, OC was able to reduce oxidative stress by decreasing lipid peroxidation and increasing antioxidant enzymes activities in serum, RBCs and liver.

Olive cake reduces glycaemia and lipemia and increases antioxidant enzymes in STZ-induced diabetes in rat erythrocytes and tissues

2019 ◽  
Vol 50 (2) ◽  
pp. 360-372
Author(s):  
Hayet Cherrad ◽  
Sherazede Bouderbala ◽  
Yahiaoui Zidan ◽  
Djamil Krouf
Keyword(s):  
Antioxidant Enzymes ◽  
Heart Muscle ◽  
Design Methodology ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Thiobarbituric Acid Reactive Substances ◽  
Olive Cake ◽  
Sod Activity ◽  
Content Type ◽  
Antioxidant Enzymes Activities

Purpose The purpose of this study is to determine the effect of olive cake (CO) on glycaemia and lipemia and lipid peroxidation and antioxidant enzymes activities in erythrocytes and tissues, in streptozotocin (STZ)-induced diabetic rats. Design/methodology/approach Diabetes was induced by a single intraperitoneal injection of STZ (55 mg/kg BW). In total, 12 diabetic D rats, weighing 260 ± 20 g, were divided into two groups fed a casein diet supplemented (D-OC) or not (D) with OC (7.5 per cent), for four weeks. Findings In D-OC compared with D, glycaemia, total cholesterol and triglycerides values (−40 per cent; p = 0.007, 27 per cent; p = 0.007 and −27 per cent; p = 0.0019). In erythrocyte, liver, kidney, heart, muscle and brain, thiobarbituric acid reactive substances contents were respectively, (−19 per cent; p = 0.03, −32 per cent; p = 0.002, −20 per cent; p = 0.04, −68 per cent; p = 0.003, −74 per cent; p = 0.0003 and −38 per cent; p = 0.04). In erythrocyte, SOD, GSH-Px and CAT activities were respectively, (+14 per cent; p = 0.01, +74 per cent; p = 0.012 and +34 per cent; p = 0.0009). In the liver, kidney, heart and muscle, SOD activity was respectively, (+31 per cent; p = 0.004, +12 per cent; p = 0.038, +43 per cent; p = 0.001 and +23 per cent; p = 0.18). GSH-Px activity was respectively, (+121 per cent; p = 0.0009, 89 per cent; p = 0.0006, + 95 per cent; p = 0.008, +71 per cent; p = 0.02 and +26 per cent; p = 0.01), in the liver, kidney, heart, muscle and brain. Catalase activity was (+21 per cent; p = 0.008) in the liver, (+88 per cent; p = 0.0002 in the kidney, +53 per cent; p = 0,002 in the heart and 83 per cent; p = 0.00004 in the muscle). Originality/value In diabetic rats, OC reduces hyperglycaemia induced by STZ and attenuates triglyceridemia and cholesterolemia. This residue is able to decrease the oxidative stress by increasing the antioxidant enzymes activity in erythrocytes and tissues. The high contents of phytoconstituents present in OC are considered to be responsible for this effect.

scholarly journals Melatonin improves diabetes-induced foetal growth retardation in rats

2015 ◽  
Vol 18 (1) ◽  
pp. 42-47
Author(s):  
LA Olayaki ◽  
AO Soladoye ◽  
OO Ojo
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Growth Retardation ◽  
Intraperitoneal Administration ◽  
Pharmaceutical Journal ◽  
Diabetic Rats ◽  
Pregnant Rats ◽  
Enzymes Activities ◽  
Foetal Growth ◽  
Antioxidant Enzymes Activities

The aim of the present study was to investigate the effect of oral melatonin administration on foetal growth retardation, utero-placental antioxidant enzymes activities and lipid peroxidation in experimental diabetic rats. Twenty pregnant rats were divided into four groups of five rats each. Diabetes mellitus was induced by a single intraperitoneal administration of 120mg/kg body weight of alloxan. From gestational day 5 to 19, 5mg/kg and 10mg/kg of oral melatonin were administered to the rats with clearly manifested gestational diabetes. On the 19th day of gestation, the rats were sacrificed by cervical dislocation and placental, foetal and uterine tissues were harvested for estimation of tissue glutathione peroxidase (GPx) activity and malondialdehyde (MDA) levels. Foetal weight, foetal size, placental and plasma glucose were also determined. Results showed that, in diabetic rats, foetal growth retardation was associated with a significant reduction in placental and uterine antioxidant enzymes (GPx) activities (P<0.001) and increased lipid peroxidation as evidenced by raised MDA concentration (P< 0.05). Treatment with oral melatonin significantly improved the foetal weight, placental and uterine antioxidant enzymes activities as well as reduced lipid peroxidation, without affecting the degree of hyperglycaemia. Effects of melatonin on foetal growth are presumed to be dependent on its ability to improve uteroplacental antioxidant enzymes activities and reduce lipid peroxidation.Bangladesh Pharmaceutical Journal 18(1): 42-47, 2015

scholarly journals The Aerobic Training and Berberine Chloride Intervention on Pancreatic Tissue Antioxidant Enzymes and Lipid Peroxidation in Type 1 Diabetic Rats

2020 ◽  
Author(s):  
Javad Ramezani ◽  
Mohammad Ali Azarbayjani ◽  
Maghsoud Peeri
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Aerobic Training ◽  
Diabetic Control ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Male Rats ◽  
P Value ◽  
Berberine Chloride

Objective: Aerobic training and berberine chloride include antioxidant characteristics. In this study, aerobic training and berberine chloride intervention on the activity of antioxidant enzymes and lipid peroxidation of pancreatic tissue were investigated in type 1 diabetic male wistar rats. Materials and Methods: In the current study, 56 Wistar male rats were randomly assigned to seven different groups (n= 8), these groups include healthy control, diabetic control, Berberine-treated diabetes (15&30 mg/kg), aerobic training-treated diabetes, Diabetes treated with Berberine and aerobic training (15&30 mg/kg). The aerobic training schedule consisted of 6 weeks treadmills with a frequency of 5 sessions per week. The Berberine was also fed a specific dose every day and a half before training. The superoxide dismutase, glutathione peroxidase, catalase, and malondialdehyde were assessed using ELISA method. Results: The results showed that aerobic training, as well as the intervention of Aerobic Training and Berberine chloride, had a significant effect on the increase of antioxidant enzymes SOD and CAT in the pancreatic tissue groups (P-value< 0.05), but did not significantly affect the GPX level (P-value> 0.05). There was a significant decrease in MDA level in all treatment groups (P-value< 0.05). In diabetic groups that received both treatments at the same time, the MDA level more decreased (P-value< 0.0005). Conclusion: The aerobic training and berberine chloride concurrent intervention have a greater effect on the antioxidant enzymes in the pancreatic tissue of diabetic specimens. Therefore, it is recommended that aerobic training be done with berberine chloride.

scholarly journals Effects of Pinus Halepensis oil on hyperuricemia, lipid peroxidation, and antioxidant enzymes activities in rat

2015 ◽  
Vol 94 (01) ◽  
pp. 27-34
Author(s):  
Nesrine GDOURA ◽  
◽  
Jean-Claude MURAT ◽  
Khansa CHAABOUNI ◽  
Fatma MAKNI AYADI ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Pinus Halepensis ◽  
Enzymes Activities ◽  
Antioxidant Enzymes Activities
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