In vivo near infra-red spectroscopy instrumentation combining spatially-resolved and intensity modulation techniques

2004 ◽  
Author(s):  
P. Rolfe
Keyword(s):  
Intensity Modulation ◽  
Spatially Resolved ◽  
Spectroscopy Instrumentation ◽  
Modulation Techniques ◽  
Infra Red

scholarly journals Automated analysis of filopodial length and spatially resolved protein concentration via adaptive shape tracking

2016 ◽  
Vol 27 (22) ◽  
pp. 3616-3626 ◽  
Author(s):  
Tanumoy Saha ◽  
Isabel Rathmann ◽  
Abhiyan Viplav ◽  
Sadhana Panzade ◽  
Isabell Begemann ◽  
...  
Keyword(s):  
Protein Concentration ◽  
Growth Dynamics ◽  
Automated Analysis ◽  
Cell Types ◽  
Control Mechanisms ◽  
Spatially Resolved ◽  
Novel Approach ◽  
Associated Proteins

Filopodia are dynamic, actin-rich structures that transiently form on a variety of cell types. To understand the underlying control mechanisms requires precise monitoring of localization and concentration of individual regulatory and structural proteins as filopodia elongate and subsequently retract. Although several methods exist that analyze changes in filopodial shape, a software solution to reliably correlate growth dynamics with spatially resolved protein concentration along the filopodium independent of bending, lateral shift, or tilting is missing. Here we introduce a novel approach based on the convex-hull algorithm for parallel analysis of growth dynamics and relative spatiotemporal protein concentration along flexible filopodial protrusions. Detailed in silico tests using various geometries confirm that our technique accurately tracks growth dynamics and relative protein concentration along the filopodial length for a broad range of signal distributions. To validate our technique in living cells, we measure filopodial dynamics and quantify spatiotemporal localization of filopodia-associated proteins during the filopodial extension–retraction cycle in a variety of cell types in vitro and in vivo. Together these results show that the technique is suitable for simultaneous analysis of growth dynamics and spatiotemporal protein enrichment along filopodia. To allow readily application by other laboratories, we share source code and instructions for software handling.

scholarly journals Spatially resolved measurements of hyperpolarized gas properties in the lung in vivo. Part I: Diffusion coefficient

1999 ◽  
Vol 42 (4) ◽  
pp. 721-728 ◽  
Author(s):  
X. Josette Chen ◽  
Harald E. M�ller ◽  
Mark S. Chawla ◽  
Gary P. Cofer ◽  
Bastiaan Driehuys ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Spatially Resolved ◽  
Hyperpolarized Gas ◽  
Gas Properties

scholarly journals Spatiotemporal co-dependency between macrophages and exhausted CD8+ T cells in cancer

2021 ◽  
Author(s):  
Kelly Kersten ◽  
Kenneth H Hu ◽  
Alexis J Combes ◽  
Bushra Samad ◽  
Tory Harwin ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Spatially Resolved ◽  
Hypoxic Conditions ◽  
Synaptic Interactions ◽  
Positive Feedback Circuit ◽  
Major Impediment

T cell exhaustion is a major impediment to anti-tumor immunity. However, it remains elusive how other immune cells in the tumor microenvironment (TME) contribute to this dysfunctional state. Here we show that the biology of tumor-associated macrophages (TAM) and exhausted T cells (Tex) in the TME is extensively linked. We demonstrate that in vivo depletion of TAM reduces exhaustion programs in tumor-infiltrating CD8+ T cells and reinvigorates their effector potential. Reciprocally, transcriptional and epigenetic profiling reveals that Tex express factors that actively recruit monocytes to the TME and shape their differentiation. Using lattice light sheet microscopy, we show that TAM and CD8+ T cells engage in unique long-lasting antigen-specific synaptic interactions that fail to activate T cells, but prime them for exhaustion, which is then accelerated in hypoxic conditions. Spatially resolved sequencing supports a spatiotemporal self-enforcing positive feedback circuit that is aligned to protect rather than destroy a tumor.

Beyond oxygen in-vivo long-wavelength near infra-red spectroscopy for hypoxia assessment

2021 ◽  
Author(s):  
Shree Krishnamoorthy ◽  
Ray Burke ◽  
Fergus McCarthy ◽  
Stefan Andersson-Engels
Keyword(s):  
Long Wavelength ◽  
Infra Red

The use of near infra-red reflectance spectroscopy (nirs) for the evaluation of forages. The application to undried silage

1992 ◽  
Vol 1992 ◽  
pp. 221-221
Author(s):  
C W Baker ◽  
D I Givens
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Agricultural Development ◽  
Reflectance Spectroscopy ◽  
Wet Chemistry ◽  
Advisory Service ◽  
Infra Red ◽  
Routine Basis

NIRS is now a familiar tool in the assessment of forage and feedingstuff quality. Recently NIRS has been applied to the direct prediction of in vivo organic matter digestibility (OMD) of grass silages (Barber et al 1990) and cereal straws (Givens et al 1991).The Agricultural Development and Advisory Service (ADAS) has been using NIRS to predict the in vivo OMD of oven dried silages on a routine basis since 1989, and it has proved to be reliable, accurate and rapid. In addition to OMD, crude protein (CP), and neutral detergent fibre (NDF), are routinely predicted by NIRS, leaving pH, ammonia, dry matter and ash still to be determined by wet chemistry methods.

Persistent luminescence induced by near infra-red photostimulation in chromium-doped zinc gallate for in vivo optical imaging

2017 ◽  
Vol 63 ◽  
pp. 51-58 ◽  
Author(s):  
Suchinder K. Sharma ◽  
Didier Gourier ◽  
Eliott Teston ◽  
Daniel Scherman ◽  
Cyrille Richard ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Persistent Luminescence ◽  
Zinc Gallate ◽  
Infra Red ◽  
In Vivo Optical Imaging
Sign in / Sign up

Export Citation Format

Share Document