This paper researches the common mode rejection ratio (CMRR) of 3 op-amp instrumentation amplifier of amplifying weak signal and presents a new calculation modal considering noise, non-ideal amplifiers, matched resistor and application restricted by the factors of affecting CMRR. At last, a instrumentation amplifier was designed and built and its measured and computed results of modal are compared. The results show that this modal presented enhances calculating precision and extends application range of instrumentation amplifier. It is valid and reasonable.