AMD's MACH family breaks PLD speed and density barrier

Author(s):  
O.P. Agrawal
Keyword(s):  
2002 ◽  
Vol 2 ◽  
pp. 1654-1656 ◽  
Author(s):  
John Graham

The standard techniques for the isolation of human peripheral blood mononuclear cells (PBMCs) using commercial “lymphocyte isolation media” cannot be satisfactorily extended to experimental animals without manipulating either the density or the osmolality of the medium. PBMCs from Macaques can also be isolated from whole blood by sedimentation on to a density barrier containing approx. 10% iodixanol, polysucrose (Ficoll) with a density of approx 1.074 g/ml.


2002 ◽  
Vol 2 ◽  
pp. 1643-1645 ◽  
Author(s):  
John Graham

Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport and the endocrine/paracrine ECL cells effectively control parietal cell function. Discontinuous gradients of iodixanol for the purification of ECL cells were subsequently simplified to the use of a density barrier. This technique is now commonly used following initial centrifugal elutriation.


2002 ◽  
Vol 2 ◽  
pp. 1540-1543
Author(s):  
John Graham

Human peripheral blood monocytes are isolated by flotation from whole blood through a single low-density barrier prepared from OptiPrep™ at 4°C. The separation from lymphocytes depends on the more rapid rate of flotation of the monocytes because of their slightly lower density and larger size. The method works optimally only with fresh (within 2 h of drawing) EDTA-anticoagulated blood. Preliminary evidence suggests that this technique may be applicable to blood from rats.


1990 ◽  
Vol 41 (5) ◽  
pp. 681-684
Author(s):  
M Y El-Ashry ◽  
D D Tskhakaya

2014 ◽  
Vol 62 ◽  
pp. 208-220 ◽  
Author(s):  
Chien-Fu Cheng ◽  
Ting-Ying Wu ◽  
Hsien-Chun Liao

2002 ◽  
Vol 2 ◽  
pp. 1607-1609 ◽  
Author(s):  
John Graham

Platelets from human blood can be isolated in high yield by centrifugation of whole blood over an iodixanol density barrier of 1.063 g/ml. The separation from all of the blood cells (which form a pellet) is based on the slower sedimentation velocity of the smaller platelets.


2002 ◽  
Vol 2 ◽  
pp. 1397-1399
Author(s):  
John Graham

From a standard plant tissue digest adjusted to a density of 1.07 g/ml, protoplasts can be harvested by flotation through a low density barrier (1.03 g/ml). The delicate nature of these bodies is suited to this flotation strategy which can be carried out at 1g.


1989 ◽  
Vol 160 ◽  
Author(s):  
K.L. Jiao ◽  
A.J. Soltyka ◽  
W.A. Anderson ◽  
S.M. Vernon

AbstractDeep level transient spectroscopy (DLTS), current-voltage- temperature (I-V-T), capacitance-voltage-temperature (C-V-T), C-T and photoreflectance spectroscopy (PRS) were used to compare the electrical properties of GaAs p-n junctions epitaxially grown by MOCVD on GaAs and Si substrates. EL2 was detected for both GaAs/GaAs and GaAs/Si structures. For GaAs/Si, a continuous distribution of trap levels was also observed which could be ascribed to misfit defects. I-V-T data revealed the saturation current of GaAs/Si diodes to be four orders in amplitude higher than for GaAs/GaAs ones. Different temperature dependences were found in saturation current density, barrier height and capacitance between the two structures. A multistep tunneling model is utilized in explaining the results for GaAs/Si. C-T measurements indicated the free carriers not to be frozen-out for GaAs/Si at the freeze-out temperature of GaAs/GaAs. A defect- induced bandgap tailing effect might be responsible for this effect, verified by PRS tests.


Sign in / Sign up

Export Citation Format

Share Document