scholarly journals In vitro quantification of lactate in Phosphate Buffer Saline (PBS) samples

2019 ◽  
Author(s):  
K Budidha ◽  
M Mamouei ◽  
N Baishya ◽  
P Vadgama ◽  
P A Kyriacou
Keyword(s):  
Phosphate Buffer ◽  
Phosphate Buffer Saline

scholarly journals Tingkat Kematangan Inti Oosit Sapi Setelah 24 Jam Presevasi Ovarium

2015 ◽  
Vol 15 (2) ◽  
pp. 72-78
Author(s):  
Rini Widyastuti ◽  
Siti Darodjah Rasad
Keyword(s):  
Phosphate Buffer ◽  
Bovine Oocyte ◽  
Oocyte Maturity ◽  
Phosphate Buffer Saline ◽  
Bovine Oocytes ◽  
Meiotic Competence

(Nuclear maturity of bovine oocyte after 24 hours ovary preservation)ABSTRACT. The objective of the research was to investigate  their meiotic competence or nuclear maturity of bovine oocytes maturity in vitro after 24 hour preservation on 5°C. Oocytes  were collected by slicing the ovaries in modified phosphate buffer saline (m-PBS). Selected cumulus-oocyte complexes (COCs) homogenous ooplasm  were cultured in maturity medium at 38°C in humidified atmosphere of 5% CO2 incubator. After 24 hours, oocytes stained for nuclear maturity’s evaluation. The proportion of oocytes at metaphase II (MII) was significantly difference (P 0.05) on oocytes that 24 hours preservated   (44.21 ± 3.04%) vs oocytes from fresh ovary (73.97 ± 9.32%) (P0.05). These results indicated that 24 hour’s preservation  bovine’s ovary on 50C  cause  decreases of nuclear oocyte maturity.

scholarly journals Investigation of surface topography of different root-end filling materials: An in vitro study

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Mine Koruyucu ◽  
Hazal Özcan ◽  
Merve Bayram ◽  
Abdullah Burak Cankaya ◽  
Nurullah Keklikoglu ◽  
...  
Keyword(s):  
Surface Topography ◽  
Normal Saline ◽  
Phosphate Buffer ◽  
Saline Solution ◽  
In Vitro Study ◽  
Phosphate Buffer Saline ◽  
Filling Materials ◽  
Retrograde Filling ◽  
Group B

Aim: Although there are many materials that can be used for retrograde filling in surgical endodontics, none of them can be regarded as an ideal material yet. The purpose of this study was to compare the surface topography of three different root-end filling materials.Methods: 36 extracted single rooted human incisor teeth were cleaned and decoronated to standardized 10 mm root lengths. The root segments were prepared and 2 mm apical resection were performed. The samples were randomly separeted to three groups (Group A: Ca(OH)2, Group B: MTA Angelus, Group C: ProRoot MTA), each comprised of 12 roots. Materials were placed as 2 mm apical barriers and obturated with guttapercha and AH-Plus sealer. Each group dimidiated two subgroups (A1,A2,B1,B2,C1,C2). Groups A1,B1,C1 were stored in normal saline (NS), groups A2,B2,C2 were stored in neutral phosphate buffer saline (NPBS) solution and samples were incubated at 370C for 2 weeks. Stereomicroscope (32X) was used to photograph the root-end filling.Results: All specimens demonstrated white crystals formation and sediment over the root-end filling materials and on the superficial border of the root-end cavities’ wall as a white plague. A2,B2,C2 samples have more crystal sediment on root-end fillings than samples A1,B1,C1. Dissolution and corrosion were observed in groups A1, A2.Conclusions: The results of this study revealed that calcium hydroxide is more resorbable than MTA Angelus and ProRoot MTA. The crystals formation and precipitation were observed in neutral phosphate buffer saline solution was more than normal saline solution for all groups as a hydroxiapatite crystals.  

Nano-Liposomal Dry Powder Inhaler of Amiloride Hydrochloride

2006 ◽  
Vol 6 (9) ◽  
pp. 3001-3009 ◽  
Author(s):  
Mahavir Bhupal Chougule ◽  
Bijay Kumar Padhi ◽  
Ambikanandan Misra
Keyword(s):  
Cystic Fibrosis ◽  
Drug Release ◽  
Phosphate Buffer ◽  
Dry Powder Inhaler ◽  
Dry Powder ◽  
Phosphate Buffer Saline ◽  
Amiloride Hydrochloride ◽  
Spray Dried

The purpose of this study was to encapsulate Amiloride Hydrochloride into nano-liposomes, incorporate it into dry powder inhaler, and to provide prolonged effective concentration in airways to enhance mucociliary clearance and prevent secondary infection in cystic fibrosis. Liposomes were prepared by thin film hydration technique and then dispersion was passed through high pressure homogenizer to achieve size of nanometer range. Nano-liposomes were separated by centrifugation and were characterized. They were dispersed in phosphate buffer saline pH 7.4 containing carriers (lactose/sucrose/mannitol), and glycine as anti-adherent. The resultant dispersion was spray dried. The spray dried powders were characterized and in vitro drug release studies were performed using phosphate buffer saline pH 7.4. in vitro and in vivo drug pulmonary deposition was carried out using Andersen Cascade Impactor and by estimating drug in bronchial alveolar lavage and lung homogenate after intratracheal instillation in rats respectively. Nano-liposomes were found to have mean volume diameter of 198 ± 15 nm, and 57% ± 1.9% of drug entrapment. Mannitol based formulation was found to have low density, good flowability, particle size of 6.7 ± 0.6 μm determined by Malvern MasterSizer, maximum fine particle fraction of 67.6 ± 0.6%, mean mass aerodynamic diameter 2.3 ± 0.1 μm, and geometric standard deviation 2.4 ± 0.1. Developed formulations were found to have prolonged drug release following Higuchi's Controlled Release model and in vivo studies showed maximal retention time of drug of 12 hrs within the lungs and slow clearance from the lungs. This study provides a practical approach for direct lung delivery of Amiloride Hydrochloride encapsulated in liposomes for controlled and prolonged retention at the site of action from dry powder inhaler. It can provide a promising alternative to the presently available nebulizers in terms of prolonged pharmacological effect, reducing systemic side effects such as potassium retention due to rapid clearance of the drug from lungs in patients suffering from cystic fibrosis.

scholarly journals PRODUKSI EMBRIO KUCING SECARA IN VITRO DARI SPERMATOZOA HASIL PRESERVASI MELALUI FERTILISASI MIKRO

2013 ◽  
Vol 7 (1) ◽  
Author(s):  
Kartini Eriani ◽  
Yuhara Sukra ◽  
Arief Boediono ◽  
Ita Djuwita ◽  
Sony Heru Sumarsono
Keyword(s):  
Phosphate Buffer ◽  
Propidium Iodine ◽  
Phosphate Buffer Saline ◽  
Intra Cytoplasmic Sperm Injection

Penelitian ini bertujuan mengetahui motilitas dan kemampuan memfertilisasi sperma kucing yang berasal dari epididimis yang disimpan pada suhu 4° C. Epididimis disimpan dalam media phosphate buffer saline (PBS) pada suhu 4 C selama 1, 3, dan 6 hari. Viabilitas spermatozoa diamati dengan pewarnaan hoechst-propidium iodine. Fungsi biologis spermatozoa dievaluasi melalui teknik kultur in vitro dengan fertilisasi mikro dan perkembangan embrio di dalam media kultur CR1aa. Hasil penelitian menunjukkan persentase spermatozoa hidup pada hari ke-1, 3, dan 6 penyimpanan masing-masing adalah 81,0; 71,7; dan 70,7% (duktus deferens), 84,0; 81,2; dan 63,2% (kauda epididimis), 84,0%; 75,0; dan 74,7% (korpus epididimis). Persentase pronukleus (PN) yang didapat dengan teknik intra cytoplasmic sperm injection (ICSI) menggunakan spermatozoa epididimis pada hari ke-1, 3, dan 6 hari penyimpanan masing-masing adalah 8,0; 10,0; dan 5,9%. Preservasi epididimis pada suhu 4 C dalam PBS dapat digunakan untuk fertilisasi dan menghasilkan embrio kucing secara in vitro.

scholarly journals Development and validation of UV spectrophotometric method for quantitative estimation of Promethazine HCl in phosphate buffer saline pH 7.4

2013 ◽  
Vol 2 (8) ◽  
pp. 141-142
Author(s):  
Dwi Nurahmanto
Keyword(s):  
Phosphate Buffer ◽  
Quantitative Estimation ◽  
Pharmaceutical Journal ◽  
Analytical Validation ◽  
Phosphate Buffer Saline ◽  
Validation Procedure ◽  
Development And Validation ◽  
Light Absorbance

A new, sensitive, rapid, simple, specific and economical procedure has been developed for determination Promethazine HCl in phosphate buffer saline pH 7.4. The purpose of this analytical validation procedure is to determine a process of assessment and to validate it by laboratory experiments to prove that the method meets the minimum standard for laboratory use. This analytical method for the determination of Promethazine HCl in phosphate buffer saline pH 7.4 can be used to estimate the amount of promethazine HCl penetrated and dissolved in the blood vessels in vitro by penetration study. The method is based on the ultraviolet light absorbance at 251 nm which is the maximum wavelength of the concerned drug. This method can be succesfully applied for determination of drug in phosphate buffer saline pH 7.4 . The results of the analysis have been validated statistically and by recovery studies.DOI: http://dx.doi.org/10.3329/icpj.v2i8.15589 International Current Pharmaceutical Journal, July 2013, 2(8): 141-142

scholarly journals Degradation behavior of theophylline/chitosan/?-cyclodextrin microspheres for pulmonary drug delivery

2016 ◽  
Vol 11 ◽  
pp. S116-S122 ◽  
Author(s):  
Li Wang ◽  
Tingting Yang ◽  
Weiping Ju ◽  
Ming Ren ◽  
Zhanqin Feng ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Phosphate Buffer ◽  
In Vitro Study ◽  
Pulmonary Drug Delivery ◽  
Degradation Behavior ◽  
Sprague Dawley ◽  
Phosphate Buffer Saline ◽  
Drug Delivery Carrier

To evaluate the degradation behavior of theophylline/chitosan/?-cyclo-dextrin microspheres, we performed both in vitro study by putting the microspheres in phosphate buffered saline or in phosphate buffer saline with enzyme and in vivo study by implanting the microspheres into the back of male Sprague-Dawley rats. The results showed that microspheres were degraded in enzymatic hydrolysis and phosphate buffer saline, which were degraded faster in 0.2 mg/mL lysozyme than in phosphate buffer saline. The morphology of microspheres in phosphate buffer saline and enzyme solution developed rough surfaces, and showed irregular shape and pores after 8 weeks. The microspheres were degraded in vivo within 8 weeks with irregular, sheet, porous morphology, and the diameters were smaller than 5 ?m. These results indicated that the theophylline/chitosan/?-cyclodextrin microspheres had a good degradation both in vitro and in vivo which can be used as a pulmonary drug delivery carrier. 

scholarly journals Determination of eight carbonyl compounds in aerosols trapped in phosphate buffer saline solutions to support in vitro assessment studies

Talanta ◽  
2018 ◽  
Vol 184 ◽  
pp. 42-49 ◽  
Author(s):  
Roberto Buratto ◽  
Daniela Correia ◽  
Monique Parel ◽  
Maude Crenna ◽  
Mickaël Bilger ◽  
...  
Keyword(s):  
Phosphate Buffer ◽  
Carbonyl Compounds ◽  
Phosphate Buffer Saline ◽  
In Vitro Assessment ◽  
Saline Solutions

Formulation and Evaluation of Microbially- triggered tablets of Valdecoxib

2010 ◽  
Vol 2 (1) ◽  
Author(s):  
Surender Verma ◽  
S. Singh ◽  
D. Mishra ◽  
Atul Gupta ◽  
Rakesh Sharma
Keyword(s):  
Phosphate Buffer ◽  
Guar Gum ◽  
Oral Route ◽  
Matrix Tablets ◽  
In Vitro Dissolution ◽  
Wet Granulation ◽  
Dissolution Study ◽  
Caecal Content ◽  
Model Drug

The objective of present study was to develop colon targeted drug delivery using bacterially triggered approach through oral route. Valdecoxib (COX-2 inhibitor) was chosen as a model drug in order to target it to colon which may prove useful in inflammatory bowel disease and related disorders. Matrix tablets of Valdecoxib were prepared by wet granulation technique utilizing different ratio of Guar gum and Sodium starch glycholate. The prepared matrix tablets were evaluated for uniformity of weight, uniformity of content, hardness and in vitro dissolution study in simulated gastric and intestinal fluid (Phosphate Buffer pH-1.2, pH-6.8 and pH-7.4), followed by Dissolution study in bio-relevant dissolution media Phosphate Buffer (pH-6.8) containing rat caecal content. The results revealed that the formulated batch had released lesser quantity of drug at pH 1.2 and pH 7.4 in 2 hors whereas in biorelevent dissolution media containing rat caecal content it released significantly higher amount of drug which was also significantly higher than the dissolution media of same pH without caecal content (microflora) and it was concluded that guar gum can be used as a potential carrier for targeting drugs to colon.

A low tech in vitro procedure using faecal liquor for the estimation of digestibility of Forages

1998 ◽  
Vol 1998 ◽  
pp. 59-59
Author(s):  
H.M. Omed ◽  
A. Faza ◽  
R.F.E. Axford ◽  
I. Ap Dewi ◽  
D.I. Givens
Keyword(s):  
Carbon Dioxide ◽  
Developing Countries ◽  
Phosphate Buffer ◽  
Two Stage ◽  
Laboratory Facilities

Two-stage techniques Tilley & Terry (1963) for the estimation of digestibility have 3 main disadvantages for use in developing countries with limited laboratory facilities. Carbon dioxide is needed to saturate the buffers; a centrifuge is necessary to separate residues from solubilised materials; and the methods uses pepsin as a proteolytic agent. The aim of the present study was to eliminate these three requirements for the faecal liquor method ( Omed et al., 1989), by replacing bicarbonate with phosphate buffer (Marten & Barnes, 1980), pepsin with biological washing liquid, and centrifugation with sedimentation.

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