Dynamics of PpIX accumulation in A549, HeLa and 3T3 cell lines

2020 ◽  
Author(s):  
D.A. Gorbenko ◽  
A.V. Belashov ◽  
T.N. Belyaeva ◽  
E.S. Kornilova ◽  
I.V. Semenova ◽  
...  
Keyword(s):  
Cell Lines ◽  
3T3 Cell Lines

Cytotoxic Activity of Major Compounds from Phaleria macrocarpa (Scheff.) Boerl. Fruits

2013 ◽  
Vol 64 (2) ◽  
Author(s):  
Siti Nur Atiqah Md Othman ◽  
Norazah Basar ◽  
Siti Pauliena Mohd Bohari
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Mouse Embryonic Fibroblast ◽  
Cytotoxic Activities ◽  
Anticancer Properties ◽  
Cervical Carcinoma Cell Line ◽  
Cell Line Hela ◽  
3T3 Cell Lines

P. macrocarpa is a well known Indonesian medicinal plant which is traditionally claimed to have anticancer properties. To date, there are numerous cytotoxic studies conducted on crude extracts of this plant. However, there are limited informations available regarding cytotoxic activity of the compounds isolated from this plant. Thus, this study investigated cytotoxic activity of two benzophenones derivatives identified as 2,6,4'-trihydroxy-4-methoxybenzophenone (1) and 6,4'-dihydroxy-4-methoxybenzophenone-2-O-β-D-glucopyranoside (2) isolated from the ethyl acetate extract. Cytotoxic activities of these compounds were performed against human cervical carcinoma cell line (HeLa) and mouse embryonic fibroblast cell line (3T3) using MTT assay. The result showed that benzophenone (1)  exhibited low cytotoxic effect against HeLa and 3T3 cell lines with IC50 values of 132 µg/ml and 158 µg/ml, repectively while benzophenone (2) was non toxic against HeLa and 3T3 cell lines are because the IC50 is more than 250 µg/ml. These findings may sheds light on the actual properties of this plant.

scholarly journals Synthesis, Antiproliferative Activity and Molecular Docking Studies of Novel Doubly Modified Colchicine Amides and Sulfonamides as Anticancer Agents

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1789 ◽  
Author(s):  
Julia Krzywik ◽  
Witold Mozga ◽  
Maral Aminpour ◽  
Jan Janczak ◽  
Ewa Maj ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Antiproliferative Activity ◽  
Docking Studies ◽  
Binding Modes ◽  
Colchicine Binding Site ◽  
3T3 Cell Lines ◽  
Colchicine Derivatives

Colchicine is a well-known compound with strong antiproliferative activity that has had limited use in chemotherapy because of its toxicity. In order to create more potent anticancer agents, a series of novel colchicine derivatives have been obtained by simultaneous modification at C7 (amides and sulfonamides) and at C10 (methylamino group) positions and characterized by spectroscopic methods. All the synthesized compounds have been tested in vitro to evaluate their cytotoxicity toward A549, MCF-7, LoVo, LoVo/DX and BALB/3T3 cell lines. Additionally, the activity of the studied compounds was investigated using computational methods involving molecular docking of the colchicine derivatives to β-tubulin. The majority of the obtained derivatives exhibited higher cytotoxicity than colchicine, doxorubicin or cisplatin against tested cancer cell lines. Furthermore, molecular modeling studies of the obtained compounds revealed their possible binding modes into the colchicine binding site of tubulin.

Influence of amide versus ester linkages on the anticancer properties of the new flavone–biotin conjugates

2019 ◽  
Vol 74 (7-8) ◽  
pp. 193-200
Author(s):  
Monika Stompor ◽  
Marta Świtalska ◽  
Agata Bajek ◽  
Joanna Wietrzyk
Keyword(s):  
Cell Lines ◽  
Amide Bond ◽  
Step Method ◽  
Fluorescent Reporter ◽  
Bifunctional Molecules ◽  
Anticancer Properties ◽  
One Step ◽  
3T3 Cell Lines ◽  
Mcf 7

Abstract Novel biotinylated C-6 substituted flavones were synthesised by a one-step method that connects biotin to 6-hydroxyflavone and 6-aminoflavone by esterification and amidation of hydroxyl and amino groups, respectively. The obtained compounds, 6-O-biotinylflavone and 6-biotinylamidoflavone, are the bifunctional molecules composed of a flavone moiety as a fluorescent reporter and biotin as a cancer-targeting unit. Antiproliferative activity was evaluated using SRB assays in MCF-7, MCF-10A, HepG2, MDA-MB-231, 4T1, and Balb/3T3 cell lines. In vitro evaluation revealed that compounds with biotin moiety displayed better cell selectivity between the cancer and normal cells than the parental substrates. These results indicate that anticancer effect is not related to the position of biotin moiety, but it is related to the presence of ester or amide bond. 6-O-Biotinylflavone was more active than 6-hydroxyflavone against human breast (MDA-MB-231) and liver (HepG2) cancer cells with IC50 (concentration of tested agent that inhibits proliferation of the cell population by 50%) values equal to 78.5 ± 18.8 μM and 133.2 ± 14.2 μM, respectively. Non biotinylated 6-aminoflavone was more active than 6-biotinylamidoflavone against all tested cell lines, with IC50 values between 34.3 ± 9.1 μM (4T1) and 173.86 ± 24.3 μM (MCF-7).

Use of Green Fluorescent Protein (GFT)-Chimeras to Study Cytoskeletal Assembly and Dynamics

1998 ◽  
Vol 4 (S2) ◽  
pp. 1010-1011
Author(s):  
G. G. Gundersen ◽  
A. Mikhailov ◽  
J. L. Martys ◽  
L. Ho ◽  
R. K. H. Liem ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Lines ◽  
Intermediate Filaments ◽  
Fluorescent Protein ◽  
Cell Structure ◽  
Β1 Integrin ◽  
Spatial Regulation ◽  
Temporal And Spatial ◽  
Green Fluorescent ◽  
3T3 Cell Lines

The cytoskeleton plays an important role in cell structure, polarity, locomotion and division. Individual elements of the cytoskeleton are composed of subunit proteins which assemble and disassemble in specific places and times within the cell. Knowledge of the temporal and spatial regulation of subunit assembly and disassembly is essential to understanding how the cytoskeleton contributes to cellular activities. The assembly and dynamics of two cytoskeletal structures, namely adhesion plaques (APs) and intermediate filaments (IFs), have been difficult to study by traditional methods. We have generated GFP-chimeras to label these structures and to study their dynamics in motile fibroblasts.To study the dynamics of APs, we prepared stable 3T3 cell lines expressing a GFP-β1 integrin chimera. The chimera was prepared by fusing GFP to the transmembrane and cytoplasmic portions of β1 intergrin, since previous studies had shown that the cytoplasmic tail of β integrins is sufficient to direct integrins to APs.

A Major Saponin from Leaves Extract of Acer velutinum

2009 ◽  
Vol 64 (9) ◽  
pp. 1081-1086 ◽  
Author(s):  
Michał Glénsk ◽  
Maciej Włodarczyk ◽  
Carla Bassarello ◽  
Cosimo Pizza ◽  
Piotr Stefanowicz ◽  
...  
Keyword(s):  
Cell Lines ◽  
In Vitro Cytotoxicity ◽  
Triterpenoid Saponin ◽  
Esi Ms ◽  
3T3 Cell Lines

A major triterpenoid saponin was isolated from the leaves extract of Acer velutinum. This compound was characterized as 21β ,22α-O-diangeloylprotoaescigenin 3-O-[β -D-glucopyranosyl- (1→2)][β -D-glucopyranosyl-(1→4)]-β -D-glucuronopyranosyl acid. Its structure was elucidated by 1H, gCOSY, ROESY, gHSQC, gHMBC NMR as well as ESI-MS experiments. Isolated saponin exhibited in vitro cytotoxicity against HL-60, B16-F0 and BALB/3T3 cell lines.

scholarly journals Production of plasminogen activator by established cell lines of mouse origin.

1977 ◽  
Vol 73 (1) ◽  
pp. 47-55 ◽  
Author(s):  
D B Rifkin ◽  
R Pollack
Keyword(s):  
Plasminogen Activator ◽  
Cell Lines ◽  
3T3 Cells ◽  
Established Cell Lines ◽  
Embryo Cultures ◽  
Established Cell ◽  
Swiss 3T3 ◽  
Swiss 3T3 Cell ◽  
Transformed Cell Lines ◽  
3T3 Cell Lines

The correlation between malignant transformation and increased plasminogen activator synthesis has been studied in a variety of established cell lines. In contrast to the behavior of secondary mouse embryo cultures, which always show increased fibrinolytic activity when transformed, no such correlation was found within the BALB/c 3T3 line and its transformed derivatives. Cell lines were established from tumors initiated in BALB/c mice by several transformed cell lines. These lines were generally found to contain no more plasminogen activator than the cells used for inoculation. A correlation was found between transformation and plasminogen activator synthesis within Swiss 3T3 cell lines. However, the correlation was not maintained by serum revertants of transformed Swiss 3T3 cells.

Nanoparticles Composed by Oligochitosan and Polyethylenimine for Gene Delivery

2013 ◽  
Vol 284-287 ◽  
pp. 418-422
Author(s):  
Chi Hsien Liu ◽  
Mei Shan Cheng
Keyword(s):  
Gene Delivery ◽  
Cell Lines ◽  
Fluorescent Protein ◽  
Synergistic Effects ◽  
Image Analyzer ◽  
Transfection Efficacy ◽  
Fluorescence Protein ◽  
Green Fluorescent ◽  
3T3 Cell Lines

Cationic carriers including polyethylenimine, liposomes, and chitosan have been used to transfer plasmid DNA in vitro by condensing anionic DNA. Here, oligochitosan (OC) was found to have capacity for in-vitro gene delivery in four cell lines. Plasmid containing green fluorescent protein (GFP) gene was used as a reporter gene. The transfection efficacy and cell viability of the transfection vehicles were analyzed by using a high-throughput image analyzer. We found that DNA polyplexes formed by high dosage of OC could be efficiently delivered into the cells. The combination of OC and polyethylenimine (PEI) were found to significantly enhance the fluorescence protein expression. The introduction of oligochitosan in PEI-mediated transfection could increase the transfection efficacy and could reduce the toxicity of PEI. Additionally, the synergistic effects of PEI and OC were confirmed in CHO, Caco2, Hep-SK, and 3T3 cell lines. The detailed mechanism of PEI and oligochitosan on transfection was investigated by using gel retardation and DNase degradation experiments. A facile and inexpensive construction of gene delivering vehicles was developed herein by using oligochitosan and PEI.

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