Resultant Radius of Curvature of Stylet-and-Tube Steerable Needles Based on the Mechanical Properties of the Soft Tissue, and the Needle

AbstractComplex biological tissues are highly viscoelastic and dynamic. Efforts to repair or replace cartilage, tendon, muscle, and vasculature using materials that facilitate repair and regeneration have been ongoing for decades. However, materials that possess the mechanical, chemical, and resorption characteristics necessary to recapitulate these tissues have been difficult to mimic using synthetic resorbable biomaterials. Herein, we report a series of resorbable elastomer-like materials that are compositionally identical and possess varying ratios of cis:trans double bonds in the backbone. These features afford concomitant control over the mechanical and surface eroding degradation properties of these materials. We show the materials can be functionalized post-polymerization with bioactive species and enhance cell adhesion. Furthermore, an in vivo rat model demonstrates that degradation and resorption are dependent on succinate stoichiometry in the elastomers and the results show limited inflammation highlighting their potential for use in soft tissue regeneration and drug delivery.

Download Full-text

Porous Cellulose-Collagen Scaffolds For Soft Tissue Regeneration: Influence of Cellulose Derivatives On Mechanical Properties And Compatibility With Adipose-Derived Stem Cells.

10.21203/rs.3.rs-1187939/v1 ◽

2022 ◽

Author(s):

Katarína Kacvinská ◽

Martina Trávničková ◽

Lucy Vojtová ◽

Petr Poláček ◽

Jana Dorazilová ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Stem Cells ◽

Cell Adhesion ◽

Soft Tissue ◽

Vascular Tissue ◽

Biological Properties ◽

Cellulose Derivatives ◽

Adipose Derived Stem Cells ◽

Soft Tissue Regeneration

Abstract This study deals with cellulose derivatives in relation to the collagen fibrils in composite collagen-cellulose scaffolds for soft tissue engineering. Two types of cellulose, i.e., oxidized cellulose (OC) and carboxymethyl cellulose (CMC), were blended with collagen (Col) to enhance its elasticity, stability and sorptive biological properties, e.g. hemostatic and antibacterial features. The addition of OC supported the resistivity of the Col fibrils in a dry environment, while in a moist environment OC caused a radical drop. The addition of CMC reduced the mechanical strength of the Col fibrils in both environments. The elongation of the Col fibrils was increased by both types of cellulose derivatives in both environments, which is closely related to tissue like behaviour. In these various mechanical environments, the ability of human adipose-derived stem cells (hADSCs) to adhere and proliferate was significantly greater in the Col and Col/OC scaffolds than in the Col/CMC scaffold. This is explained by deficient mechanical support and loss of stiffness due to the high swelling capacity of CMC. Although Col/OC and Col/CMC acted differently in terms of mechanical properties, both materials were observed to be cytocompatible, with varying degrees of further support for cell adhesion and proliferation. While Col/OC can serve as a scaffolding material for vascular tissue engineering and for skin tissue engineering, Col/CMC seems to be more suitable for moist wound healing, e.g. as a mucoadhesive gel for exudate removal, since there was almost no cell adhesion.

Download Full-text

Mimicked scaffolds based on coated silk woven fabric with gelatin and chitosan for soft tissue defect in oral maxillofacial area

The International Journal of Artificial Organs ◽

10.1177/0391398819877191 ◽

2019 ◽

Vol 43 (3) ◽

pp. 189-202

Author(s):

Supaporn Sangkert ◽

Suttatip Kamolmatyakul ◽

Jirut Meesane

Keyword(s):

Mechanical Properties ◽

Cell Proliferation ◽

Soft Tissue ◽

Physical Performance ◽

Silk Fibroin ◽

Swelling Behavior ◽

Woven Fabric ◽

Soft Tissue Reconstruction ◽

Residual Water ◽

Defect Sites

Soft tissue defects in the oral maxillofacial area are critical problems for many patients and, in some cases, patients require an operation coupled with a performance scaffold substitution. In this research, mimicked anatomical scaffolds were constructed using gelatin- and chitosan-coated woven silk fibroin fabric. The morphologies, crystals, and structures were observed and then characterized using scanning electron microscopy, X-ray diffraction, and differential scanning calorimetry, respectively. Physical performance was evaluated from the swelling behavior, mechanical properties, and biodegradation, while the biological performance was tested with fibroblasts and keratinocytes, after which cell proliferation, viability, and histology were evaluated. The results revealed that a coated woven silk fibroin fabric displayed a crystal structure of silk fibroin with amorphous gelatin and chitosan layers. Also, the coated fabrics contained residual water within their structure. The physical performance of the coated woven silk fibroin fabric with gelatin showed suitable swelling behavior and mechanical properties along with acceptable biodegradation for insertion at a defect site. The biological performances including cell proliferation, viability, and histology were suitable for soft tissue reconstruction at the defect sites. Finally, the results demonstrated that mimicked anatomical scaffolds based on a gelatin layer on woven silk fibroin fabric had the functionality that was promising for soft tissue construction in oral maxillofacial defect.

Download Full-text

Development of Photocrosslinkable Urethane-Doped Polyester Elastomers for Soft Tissue Engineering

International Journal of Biomaterials Research and Engineering ◽

10.4018/ijbre.2011010102 ◽

2011 ◽

Vol 1 (1) ◽

pp. 18-31 ◽

Cited By ~ 10

Author(s):

Yi Zhang ◽

Richard T. Tran ◽

Dipendra Gyawali ◽

Jian Yang

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Soft Tissue ◽

Hexamethylene Diisocyanate ◽

Elongation At Break ◽

Thermally Induced ◽

Molar Ratios ◽

3D Network ◽

Soft Tissue Engineering

Finding an ideal biomaterial with the proper mechanical properties and biocompatibility has been of intense focus in the field of soft tissue engineering. This paper reports on the synthesis and characterization of a novel crosslinked urethane-doped polyester elastomer (CUPOMC), which was synthesized by reacting a previously developed photocrosslinkable poly (octamethylene maleate citrate) (POMC) prepolymers (pre-POMC) with 1,6-hexamethylene diisocyanate (HDI) followed by thermo- or photo-crosslinking polymerization. The mechanical properties of the CUPOMCs can be tuned by controlling the molar ratios of pre-POMC monomers, and the ratio between the prepolymer and HDI. CUPOMCs can be crosslinked into a 3D network through polycondensation or free radical polymerization reactions. The tensile strength and elongation at break of CUPOMC synthesized under the known conditions range from 0.73±0.12MPa to 10.91±0.64MPa and from 72.91±9.09% to 300.41±21.99% respectively. Preliminary biocompatibility tests demonstrated that CUPOMCs support cell adhesion and proliferation. Unlike the pre-polymers of other crosslinked elastomers, CUPOMC pre-polymers possess great processability demonstrated by scaffold fabrication via a thermally induced phase separation method. The dual crosslinking methods for CUPOMC pre-polymers should enhance the versatile processability of the CUPOMC used in various conditions. Development of CUPOMC should expand the choices of available biodegradable elastomers for various biomedical applications such as soft tissue engineering.

Download Full-text

Design and additive manufacturing of flexible polycaprolactone scaffolds with highly-tunable mechanical properties for soft tissue engineering

Materials & Design ◽

10.1016/j.matdes.2020.108508 ◽

2020 ◽

Vol 189 ◽

pp. 108508 ◽

Cited By ~ 5

Author(s):

Zijie Meng ◽

Jiankang He ◽

Zhihao Cai ◽

Fengping Wang ◽

Juliang Zhang ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Additive Manufacturing ◽

Soft Tissue ◽

Soft Tissue Engineering ◽

Tunable Mechanical Properties

Download Full-text

Engineering approaches for characterizing soft tissue mechanical properties: A review

Clinical Biomechanics ◽

10.1016/j.clinbiomech.2019.07.016 ◽

2019 ◽

Vol 69 ◽

pp. 127-140 ◽

Cited By ~ 4

Author(s):

Alekya B. ◽

Sanjay Rao ◽

Hardik J. Pandya

Keyword(s):

Mechanical Properties ◽

Soft Tissue

Download Full-text

Reliability Investigation of Tissue Resonator Indenter Device

ASME 2010 Dynamic Systems and Control Conference, Volume 2 ◽

10.1115/dscc2010-4073 ◽

2010 ◽

Author(s):

Ming Jia ◽

Jean W. Zu ◽

Alireza Hariri

Keyword(s):

Mechanical Properties ◽

Soft Tissue ◽

Soft Tissues ◽

Tissue Properties ◽

University Of Toronto ◽

In Vivo Measurements ◽

Disease Diagnostics ◽

Working Principle ◽

The University

Knowledge of tissue mechanical properties is widely required by medical applications, such as disease diagnostics, surgery operation, simulation, planning, and training. A new portable device, called Tissue Resonator Indenter Device (TRID), has been developed for measurement of regional viscoelastic properties of soft tissues at the Bio-instrument and Biomechanics Lab of the University of Toronto. As a device for soft tissue properties in-vivo measurements, the reliability of TRID is crucial. This paper presents TRID’s working principle and the experimental study of TRID’s reliability with respect to inter-reliability, intra-reliability, and the indenter misalignment effect as well. The experimental results show that TRID is a reliable device for in-vivo measurements of soft tissue mechanical properties.

Download Full-text