Acoustical Streaming in Microfluidic CMUT Integrated Chip Controls the Biochemical Interaction Rate

2017 ◽  
Vol 26 (5) ◽  
pp. 1012-1017 ◽  
Author(s):  
Donatas Pelenis ◽  
Dovydas Barauskas ◽  
Evaldas Sapeliauskas ◽  
Gailius Vanagas ◽  
Marius Mikolajunas ◽  
...  
Keyword(s):  
Interaction Rate ◽  
Biochemical Interaction

scholarly journals Lost in the stream? Professional efficacy perceptions of journalists in the context of dark participation

Journalism ◽  
2021 ◽  
pp. 146488492110169
Author(s):  
Florian Wintterlin ◽  
Klara Langmann ◽  
Svenja Boberg ◽  
Lena Frischlich ◽  
Tim Schatto-Eckrodt ◽  
...  
Keyword(s):  
Online Communication ◽  
Perceived Efficacy ◽  
Professional Efficacy ◽  
Interaction Rate ◽  
Perceived Credibility ◽  
Deliberative Discourse ◽  
Online Comments ◽  
Depth Interviews

Online comments and contributions from users are not always constructive nor rational. This also applies to content that is directed at journalists or published on journalistic platforms. So-called ‘dark participation’ in online communication is a challenge that journalists have to face because it lowers users’ perceived credibility of media brands and hinders a deliberative discourse in comment sections. This study examines how journalists perceive themselves in relation to dark participation, what measures they take against it, and how they assess the efficacy of these measures. Based on in-depth interviews ( N = 26), we find that journalists overall considered themselves to be effective in handling dark participation. The perceived efficacy differed according to the grade of engagement with users. Journalists who interacted very much or very little with users perceive the efficacy of their interventions to be highest, whilst those with medium levels of interaction rate their efficacy to be lower. Furthermore, the perceived amount of dark participation also affected the perceived efficacy.

scholarly journals Biochemical and Structural Characterization of Cross-Linked Enzyme Aggregates (CLEAs) of Organic Solvent Tolerant Protease

Catalysts ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 55 ◽  
Author(s):  
Muhammad Syafiq Mohd Razib ◽  
Raja Noor Zaliha Raja Abd Rahman ◽  
Fairolniza Mohd Shariff ◽  
Mohd Shukuri Mohamad Ali
Keyword(s):  
Structural Analysis ◽  
Organic Solvent ◽  
Average Diameter ◽  
Relative Activity ◽  
Biochemical Properties ◽  
Biochemical Interaction ◽  
Organic Solvent Tolerant ◽  
Random Aggregate ◽  
Solvent Tolerant ◽  
The Stability

Cross-linked enzyme aggregates (CLEAs) is an immobilization technique that can be used to customize enzymes under an optimized condition. Structural analysis on any enzyme treated with a CLEA remains elusive and has been less explored. In the present work, a method for preparing an organic solvent tolerant protease using a CLEA is disclosed and optimized for better biochemical properties, followed by an analysis of the structure of this CLEA-treated protease. The said organic solvent tolerant protease is a metalloprotease known as elastase strain K in which activity of the metalloprotease is measured by a biochemical interaction with azocasein. Results showed that when a glutaraldehyde of 0.02% (v/v) was used under a 2 h treatment, the amount of recovered activity in CLEA-elastase was highest. The recovered activity of CLEA-elastase and CLEA-elastase-SB (which was a CLEA co-aggregated with starch and bovine serum albumin (BSA)) were at an approximate 60% and 80%, respectively. The CLEA immobilization of elastase strain K allowed the stability of the enzyme to be enhanced at high temperature and at a broader pH. Both CLEA-elastase and CLEA-elastase-SB end-products were able to maintain up to 67% enzyme activity at 60 °C and exhibiting an enhanced stability within pH 5–9 with up to 90% recovering activity. By implementing a CLEA on the organic solvent tolerant protease, the characteristics of the organic solvent tolerant were preserved and enhanced with the presence of 25% (v/v) acetonitrile, ethanol, and benzene at 165%, 173%, and 153% relative activity. Structural analysis through SEM and dynamic light scattering (DLS) showed that CLEA-elastase had a random aggregate morphology with an average diameter of 1497 nm.

Biochemical Interaction Analysis on ATR Devices:  A Wet Chemistry Approach for Surface Functionalization

Langmuir ◽  
2007 ◽  
Vol 23 (2) ◽  
pp. 949-955 ◽  
Author(s):  
M. Voue ◽  
E. Goormaghtigh ◽  
F. Homble ◽  
J. Marchand-Brynaert ◽  
J. Conti ◽  
...  
Keyword(s):  
Surface Functionalization ◽  
Interaction Analysis ◽  
Wet Chemistry ◽  
Biochemical Interaction

scholarly journals The Interaction Rate in Holographic Models of Dark Energy

2009 ◽  
Author(s):  
Diego Pavón ◽  
Anjan A. Sen ◽  
Kerstin E. Kunze ◽  
Marc Mars ◽  
Miguel Angel Vázquez-Mozo
Keyword(s):  
Dark Energy ◽  
Interaction Rate

scholarly journals Effect of Cassia occidentalis on Parthenium hysterophorus by way of secondary metabolites

2016 ◽  
Vol 5 (11) ◽  
pp. 1451
Author(s):  
Jai Knox
Keyword(s):  
Dry Weight ◽  
Suppressive Effect ◽  
Significant Inhibition ◽  
Parthenium Hysterophorus ◽  
Biological Parameters ◽  
Fresh Weight ◽  
Cassia Occidentalis ◽  
Medicinal Value ◽  
Biochemical Interaction ◽  
Relative Water

Cassia occidentalis is an extensive growing weed in India with some medicinal value. It has been reported to show strong biochemical interaction causing inhibition of Parthenium hysterophorus which is an obnoxious weed of today. The present study was performed to confirm its inhibition on Parthenium. Biological parameters like Seed germination, Germination velocity index, Shoot length and Root length of 10 days old Parthenium seedlings was recorded. Fresh weight (FW), Dry weight (DW) and Relative water content% (RWC) was recorded after treating the mature Parthenium plant with various aqueous leaf leachates of Cassia occidentalis. Aqueous leaf leachates of Cassia occidentalis which were prepared in different concentrations showed significant inhibition in all biological parameters on Parthenium. This study gives a strong clue regarding the potential of suppressive effect of allelochemicals present in Cassia on Parthenium.

BIOMEDICAL ENGINEERING OF SCLEROSTIN ACTION IN THE BONE REMODELING

2021 ◽  
pp. 2150016
Author(s):  
Alessandro Nutini ◽  
Ayesha Sohail ◽  
Shabieh Farwa
Keyword(s):  
Bone Remodeling ◽  
Wnt Pathway ◽  
Bone Cancer ◽  
Theoretical Research ◽  
Wnt Signal Pathway ◽  
Biochemical Interaction ◽  
Conserved Genes ◽  
Two Phases ◽  
Wnt Signal

Mathematical modeling of biological processes has bridged the fields of experimental as well as theoretical research and has carried forward remarkable innovation. Sclerostin is a fundamental communication element for bone remodeling and its activity regulates the reabsorption and deposition of new bone tissue. During this research, we have presented several studies, which illustrate the function of sclerostin in communication with the Wnt signaling pathways. This article features the sclerostin-based pathological patterns related to diseases such as bone cancer. To have a good remodeling process, the osteocytes must recruit the pre-osteoblast cells from the mesenchymal stem cells with the help of the signal mechanism given by the Wnt pathway. The Wnt signal pathway is a complex transduction of a pool of well-conserved genes whose expression regulates various activities like gene translation, cell adhesion, cell differentiation, mitogenic stimulation and polarity cell. The complexity of the interaction of the Wnt pathway is due to the ligands of Wnt itself, to the proteins R-spondin and norrin. The receptors on the surface of the cell, then, activate a process of transduction of the intracellular signal that initiates gene transduction. The hypothesis of a sort of “steady state” has therefore proved indispensable to establish a sort of common base on which the two phases. This paper seeks to give a qualitative view of the action of sclerostin through a simple mathematical model. We use a logic related to stimulation and inhibition signals of new tissue production and illustrate the role of sclerostin in the mechanical and biochemical interaction during the bone remodeling process.

Enhancement of the Inelastic Nuclear Interaction Rate in Crystals via Antichanneling

2019 ◽  
Vol 123 (4) ◽  
Author(s):  
E. Bagli ◽  
D. De Salvador ◽  
L. Bacci ◽  
F. Sgarbossa ◽  
L. Bandiera ◽  
...  
Keyword(s):  
Nuclear Interaction ◽  
Interaction Rate

scholarly journals Mertk Interacts with Tim-4 to Enhance Tim-4-Mediated Efferocytosis

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1625 ◽  
Author(s):  
Byeongjin Moon ◽  
Juyeon Lee ◽  
Sang-Ah Lee ◽  
Chanhyuk Min ◽  
Hyunji Moon ◽  
...  
Keyword(s):  
Cell Surface ◽  
Molecular Mechanism ◽  
Soluble Form ◽  
Physical Interaction ◽  
Apoptotic Cells ◽  
Type Iii ◽  
Biochemical Interaction ◽  
Fibronectin Type Iii ◽  
Fibronectin Type Iii Domain ◽  
Fibronectin Type

Apoptotic cells expressing phosphatidylserine (PS) on their cell surface are directly or indirectly recognized by phagocytes through PS-binding proteins. The PS-binding protein Tim-4 secures apoptotic cells to phagocytes to facilitate the engulfment of apoptotic cells. However, the molecular mechanism by which Tim-4 transduces signals to phagocytes during Tim-4-mediated efferocytosis is incompletely understood. Here, we report that Tim-4 collaborates with Mertk during efferocytosis through a biochemical interaction with Mertk. Proximal localization between the two proteins in phagocytes was observed by immunofluorescence and proximal ligation assays. Physical association between Tim-4 and Mertk, which was mediated by an interaction between the IgV domain of Tim-4 and the fibronectin type-III domain of Mertk, was also detected with immunoprecipitation. Furthermore, the effect of Mertk on Tim-4-mediated efferocytosis was abolished by GST-MertkFnIII, a soluble form of the fibronectin type-III domain of Mertk that disrupts the interaction between Tim-4 and Mertk. Taken together, the results from our study suggest that a physical interaction between Tim-4 and Mertk is necessary for Mertk to enhance efferocytosis mediated by Tim-4.

Sign in / Sign up

Export Citation Format

Share Document