Automatic and Robust Estimation of Heart Rate in Zebrafish Larvae

2018 ◽  
Vol 15 (3) ◽  
pp. 1041-1052 ◽  
Author(s):  
Syam Krishna ◽  
Kiranam Chatti ◽  
Ramesh R. Galigekere
Keyword(s):  
Heart Rate ◽  
Robust Estimation ◽  
Zebrafish Larvae

scholarly journals Robust estimation of fetal heart rate from US Doppler signals

2010 ◽  
Vol 3 (1) ◽  
pp. 691-699 ◽  
Author(s):  
Iulian Voicu ◽  
Jean-Marc Girault ◽  
Catherine Roussel ◽  
Aliette Decock ◽  
Denis Kouame
Keyword(s):  
Heart Rate ◽  
Robust Estimation ◽  
Fetal Heart Rate ◽  
Fetal Heart ◽  
Doppler Signals

scholarly journals Acute and Sub-Chronic Exposure to Artificial Sweeteners at the Highest Environmentally Relevant Concentration Induce Less Cardiovascular Physiology Alterations in Zebrafish Larvae

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 548
Author(s):  
Ferry Saputra ◽  
Yu-Heng Lai ◽  
Rey Arturo T. Fernandez ◽  
Allan Patrick G. Macabeo ◽  
Hong-Thih Lai ◽  
...  
Keyword(s):  
Heart Rate ◽  
Calorie Intake ◽  
Artificial Sweeteners ◽  
Cardiovascular Physiology ◽  
Artificial Sweetener ◽  
Dopamine Level ◽  
Food Ingredients ◽  
Relevant Concentration ◽  
Zebrafish Larvae ◽  
Cardiovascular Performance

Artificial sweeteners are widely used food ingredients in beverages and drinks to lower calorie intake which in turn helps prevent lifestyle diseases such as obesity. However, as their popularity has increased, the release of artificial sweetener to the aquatic environment has also increased at a tremendous rate. Thus, our study aims to systematically explore the potential cardiovascular physiology alterations caused by eight commercial artificial sweeteners, including acesulfame-K, alitame, aspartame, sodium cyclamate, dulcin, neotame, saccharine and sucralose, at the highest environmentally relevant concentration on cardiovascular performance using zebrafish (Danio rerio) as a model system. Embryonic zebrafish were exposed to the eight artificial sweeteners at 100 ppb and their cardiovascular performance (heart rate, ejection fraction, fractional shortening, stroke volume, cardiac output, heartbeat variability, and blood flow velocity) was measured and compared. Overall, our finding supports the safety of artificial sweetener exposure. However, several finding like a significant increase in the heart rate and heart rate variability after incubation in several artificial sweeteners are noteworthy. Biomarker testing also revealed that saccharine significantly increase the dopamine level in zebrafish larvae, which is might be the reason for the cardiac physiology changes observed after saccharine exposure.

scholarly journals Phytochemical analysis of ionic liquid-Graviola (Annona muricata) fruit extract and its acute toxicity on zebrafish early-life stages

2020 ◽  
pp. 113-124
Author(s):  
Djabir Daddiouaissa ◽  
Azura Amid ◽  
Syahida Ahmad ◽  
Ahmed A. M. Elnour
Keyword(s):  
Heart Rate ◽  
Ionic Liquid ◽  
Acute Toxicity ◽  
Biological Activities ◽  
Preliminary Finding ◽  
Herbal Product ◽  
Phytochemical Analysis ◽  
Annona Muricata ◽  
Fruit Extract ◽  
Zebrafish Larvae

Annona muricata, commonly known as soursop and Graviola, is a member of the Annonaceae family. Some of its phytochemicals were reported to have a neurotoxicity effect causing neurodegenerative diseases. However, different parts of this tree have been used for ages in traditional medicine due to their biological activities, such as anti-inflammatory, antimicrobial, antioxidant and anticancer effects. This study aimed to qualitatively screen the crude ionic liquid-Graviola fruit extract (IL-GFE) phytochemical composition, assess its acute toxicity and determine the lethal concentrations using zebrafish (Danio rerio) embryos. IL-GFE contains acetogenins, alkaloids, phenols, terpenoids, tannins and flavonoids. Acute toxicity effects of IL-GFE on zebrafish embryos were observed from 24 to 120 hours of post fertilisation (hpf). The survival rate, LC50, sublethal endpoints and effect of IL-GFE on the heart rate of zebrafish larvae were assessed. Results showed that the lethal concentration (LC50) of the crude IL-GFE was 173.45 μg/mL. Interestingly, no significant changes on the morphology of the treated zebrafish were observed at a concentration of 125 μg/mL. However, the heart rate of zebrafish larvae at 96 hpf was significantly decreased by 33.76% after treated with crude IL-GFE at 125 µg/mL (119.00 ± 4.72 beats/min) as compared to the untreated group (179.67 ± 4.66 beats/min). This preliminary finding showed that crude ionic liquid-Graviola fruit extract and its phytoconstituents might have the potential to be developed as a food supplement or herbal product. However, further tests need to be conducted to evaluate its medicinal properties and adverse effects on organisms of higher orders.

Influence of hypoxia and of hypoxemia on the development of cardiac activity in zebrafish larvae

2002 ◽  
Vol 283 (4) ◽  
pp. R911-R917 ◽  
Author(s):  
E. Jacob ◽  
M. Drexel ◽  
T. Schwerte ◽  
B. Pelster
Keyword(s):  
Heart Rate ◽  
Cardiac Output ◽  
Oxygen Transport ◽  
Chronic Hypoxia ◽  
Cardiac Activity ◽  
Environmental Water ◽  
Whole Body ◽  
Zebrafish Larvae ◽  
Hypoxic Conditions ◽  
Hatching Time

Cardiac activity and anaerobic metabolism were analyzed in zebrafish larvae raised under normoxia (Po 2 = 20 kPa) and under chronic hypoxia (Po 2 = 10 kPa) at three different temperatures (25, 28, and 31°C). Heart rate increased with development and with temperature. Under normoxia, cardiac output increased significantly at high temperature (31°C), but not at 28 or at 25°C. Under chronic hypoxia, however, heart rate as well as cardiac output increased at all temperatures in larvae at about hatching time or shortly thereafter. Cardiac activity of larvae raised for 2 wk after fertilization with a reduced hemoglobin oxygen-carrying capacity in their blood (hypoxemia; due to the presence of CO or of phenylhydrazine in the incubation water) was not different from control animals. Whole body lactate content of these animals did not increase. Thus there was no indication of a stimulated anaerobic energy metabolism. The increase in cardiac activity observed during hypoxia suggests that at about hatching time receptors are present that sense hypoxic conditions, and this information can be used to induce a stimulation of convective oxygen transport to compensate for a reduction in bulk oxygen diffusion in the face of a reduced oxygen gradient between environmental water and tissues. Under normoxia, however, the Po 2 gradient between environmental water and tissues and diffusional oxygen transport assure sufficient oxygen supply even if hemoglobin oxygen transport in the blood is severely impaired. Thus, under normoxic conditions and with a normal metabolic rate of the tissues, convective oxygen transport is not required until ∼2 wk after fertilization.

scholarly journals In vivo Modelling of Toxicity of Eight Commercial Artificial Sweeteners in Daphnia Neonates and Zebrafish Embryos through Cardiac Performance Assessments

2020 ◽  
Author(s):  
Ferry Saputra ◽  
Yu-Heng Lai ◽  
Rey Arturo Fernandez ◽  
Allan Patrick G. Macabeo ◽  
Hong-Thih Lai ◽  
...  
Keyword(s):  
Heart Rate ◽  
Adverse Effects ◽  
Cardiac Performance ◽  
Calorie Intake ◽  
Artificial Sweeteners ◽  
Artificial Sweetener ◽  
Food Ingredients ◽  
Zebrafish Larvae ◽  
Sodium Cyclamate

Artificial sweeteners are widely used food ingredients in beverages and drinks to lower calorie intake which in turn prevent lifestyle diseases such as obesity. Epidemiological evidences suggest that an overdose of artificial sweeteners could result to adverse effects after consumption. Thus, our study aims to systematically explore the potential adverse effects of eight commercial artificial sweeteners, including acesulfame-K, alitame, aspartame, sodium cyclamate, dulcin, neotame, saccharin and sucralose on cardiac performances of zebrafish (Danio rerio) and Daphnia as model animals. Embryonic zebrafish and Daphnia were exposed to eight artificial sweeteners at 100 ppb concentrations and their cardiac performance (heart rate, ejection fraction, fractional shortening, stroke volume, cardiac output and heartbeat regularity) were measured and compared. Saccharin significantly increased the heart rate of zebrafish larvae while a significant decrease was observed in Daphnia. Significant increase was also noted in zebrafish heart rate variability after incubation in acesulfame K, dulcin, sodium cyclamate, and sucralose. However, a significant increase in Daphnia was only observed after incubation in dulcin. Based on Principal Component Analysis (PCA) and hierarchical clustering results, several artificial sweetener samples were species-specific to zebrafish and Daphnia. Our study demonstrates the potential adverse physiological effects of artificial sweeteners in cardiovascular systems of zebrafish larvae and Daphnia.

scholarly journals Edible additive effects on zebrafish cardiovascular functionality with hydrodynamic assessment

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yu-Fang Wang ◽  
I.-Wei Chen ◽  
Satishkumar Subendran ◽  
Chun-Wei Kang ◽  
Bivas Panigrahi ◽  
...  
Keyword(s):  
Heart Rate ◽  
Juvenile Fish ◽  
Brilliant Blue ◽  
Toxicity Screening ◽  
Pectoral Fin ◽  
Promising Alternative ◽  
Practical Applications ◽  
Zebrafish Larvae ◽  
Brilliant Blue Fcf

Abstract Food coloring is often used as a coloring agent in foods, medicines and cosmetics, and it was reported to have certain carcinogenic and mutagenic effects in living organisms. Investigation of physiological parameters using zebrafish is a promising methodology to understand disease biology and drug toxicity for various drug discovery on humans. Zebrafish (Danio rerio) is a well-acknowledged model organism with combining assets such as body transparency, small size, low cost of cultivation, and high genetic homology with humans and is used as a specimen tool for the in-vivo throughput screening approach. In addition, recent advances in microfluidics show a promising alternative for zebrafish manipulation in terms of drug administration and extensive imaging capability. This pilot work highlighted the design and development of a microfluidic detection platform for zebrafish larvae through investigating the effects of food coloring on cardiovascular functionality and pectoral fin swing ability. The zebrafish embryos were exposed to the Cochineal Red and Brilliant Blue FCF pigment solution in a concentration of (0.02‰, 0.2‰) cultured in the laboratory from the embryo stage to hatching and development until 9 days post fertilization (d.p.f.). In addition, zebrafish swimming behaviors in terms of pectoral fin beating towards the toxicity screening were further studied by visualizing the induced flow field. It was evidenced that Cochineal Red pigment at a concentration of 0.2‰ not only significantly affected the zebrafish pectoral fin swing behavior, but also significantly increased the heart rate of juvenile fish. The higher concentration of Brilliant Blue FCF pigment (0.2%) increased heart rate during early embryonic stages of zebrafish. However, zebrafish exposed to food coloring did not show any significant changes in cardiac output. The applications of this proposed platform can be further extended towards observing the neurobiological/hydrodynamic behaviors of zebrafish larvae for practical applications in drug tests.

scholarly journals An OpenCV-Based Approach for Automated Cardiac Rhythm Measurement in Zebrafish from Video Datasets

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1476
Author(s):  
Ali Farhan ◽  
Kevin Adi Kurnia ◽  
Ferry Saputra ◽  
Kelvin H.-C. Chen ◽  
Jong-Chin Huang ◽  
...  
Keyword(s):  
Heart Rate ◽  
Cardiac Rhythm ◽  
Region Of Interest ◽  
Heart Rhythm ◽  
Transgenic Zebrafish ◽  
Established Method ◽  
Zebrafish Larvae ◽  
Observation Methods ◽  
Good Consistency ◽  
User Friendly

Cardiac arrhythmia has been defined as one of the abnormal heart rhythm symptoms, which is a common problem dealt with by cardiologists. Zebrafish were established as a powerful animal model with a transparent body that enables optical observation to analyze cardiac morphology and cardiac rhythm regularity. Currently, research has observed heart-related parameters in zebrafish, which used different approaches, such as starting from the use of fluorescent transgenic zebrafish, different software, and different observation methods. In this study, we developed an innovative approach by using the OpenCV library to measure zebrafish larvae heart rate and rhythm. The program is designed in Python, with the feature of multiprocessing for simultaneous region-of-interest (ROI) detection, covering both the atrium and ventricle regions in the video, and was designed to be simple and user-friendly, having utility even for users who are unfamiliar with Python. Results were validated with our previously published method using ImageJ, which observes pixel changes. In summary, the results showed good consistency in heart rate-related parameters. In addition, the established method in this study also can be widely applied to other invertebrates (like Daphnia) for cardiac rhythm measurement.

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