Multitarget protection of Pterospartum tridentatum phenolic‐rich extracts against a wide range of free radical species, antidiabetic activity and effects on human colon carcinoma (Caco‐2) cells

2020 ◽  
Vol 85 (12) ◽  
pp. 4377-4388
Author(s):  
Ana Carolina Gonçalves ◽  
Catarina Bento ◽  
Ana R. Nunes ◽  
Manuel Simões ◽  
Gilberto Alves ◽  
...  
Keyword(s):  
Free Radical ◽  
Colon Carcinoma ◽  
Human Colon ◽  
Antidiabetic Activity ◽  
Radical Species ◽  
Human Colon Carcinoma ◽  
Free Radical Species ◽  
Wide Range

scholarly journals Cytotoxic and apoptotic activity of Canavalia seed extract in HT-29 human colon carcinoma cells

Biomedicine ◽  
2021 ◽  
Vol 41 (1) ◽  
pp. 104-111
Author(s):  
Geetha Suvarna ◽  
Ashwini Prabhu ◽  
Katheeja Muhseena N. ◽  
Bhagya B. Sharma
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Colon Carcinoma ◽  
Structural Similarity ◽  
Human Colon ◽  
Dependent Manner ◽  
Human Colon Carcinoma ◽  
Wide Range ◽  
Ic50 Values ◽  
Ht 29

Introduction and Aim: Concanavalin the lectin from Canavalia spp. share high structural similarity among the same taxon and exhibit wide range of biological functions. The antiproliferative activity of lectins ConA (C. ensiformis), ConC (C. cathartica), ConG (C. gladiata) and ConM (C. rosea) were studied in human colon carcinoma.     Materials and Methods: Human colon carcinoma HT-29 cells and human normal kidney HEK293T cells were examined. The cytotoxicity was evaluated by MTT assay. Cell apoptosis was analyzed by AO-EB (acridine orange-ethidium bromide dual staining) method. Cell cycle was investigated by flow cytometry (RNase-propidium iodide-based flow cytometry). The expression of cell signaling genes was analyzed by qRT-PCR.   Results: All concanavalins significantly inhibited the proliferation of HT-29 cells in dose-dependent manner (IC50 values were ConG-10.45, ConA-14.86, ConC-33.34 and ConM-48.98 µg/mL respectively). ConA and ConG induced apoptotic morphology in HT-29 cells without affecting HEK293T cells. Similarly, both lectins increased the sub-G0/G1 proportions in HT-29 cells dose-dependently. ConG showed down-regulation of AKT1, ERK1/2 and mutant p53 in HT-29 cells.     Conclusion: Concanavalin extract is shown to possess cytotoxic effect through inducing apoptosis, especially causing cell cycle arrest at G0/G1 phase in HT-29 cells.

The influence of extracts from Rubus caesius leaves on human colon carcinoma cells cultured in vitro

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
R Paduch ◽  
M Tomczyk ◽  
A Wiater ◽  
A Dudek ◽  
M Pleszczynska ◽  
...  
Keyword(s):  
Colon Carcinoma ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma ◽  
Cells Cultured

Modulation of EGF Receptor Expression by Differentiating Agents in Human Colon Carcinoma Cell Lines

1990 ◽  
Vol 2 (10) ◽  
pp. 345-355 ◽  
Author(s):  
Lizabeth Deutsch Murphy ◽  
Eva M. Valverius ◽  
Maria Tsokos ◽  
Lyn A. Mickley ◽  
Neal Rosen ◽  
...  
Keyword(s):  
Colon Carcinoma ◽  
Cell Lines ◽  
Egf Receptor ◽  
Carcinoma Cell ◽  
Human Colon ◽  
Receptor Expression ◽  
Human Colon Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Human Colon Carcinoma ◽  
Differentiating Agents

scholarly journals Fatty acylation of heparan sulfate proteoglycan from human colon carcinoma cells.

1990 ◽  
Vol 265 (32) ◽  
pp. 19980-19989
Author(s):  
R V Iozzo ◽  
I Kovalszky ◽  
N Hacobian ◽  
P K Schick ◽  
J S Ellingson ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Colon Carcinoma ◽  
Human Colon ◽  
Heparan Sulfate Proteoglycan ◽  
Carcinoma Cells ◽  
Sulfate Proteoglycan ◽  
Fatty Acylation ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma

scholarly journals Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells

2009 ◽  
Vol 15 (9) ◽  
pp. 1079 ◽  
Author(s):  
Piet Habbel ◽  
Karsten H Weylandt ◽  
Katja Lichopoj ◽  
Johannes Nowak ◽  
Martin Purschke ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Docosahexaenoic Acid ◽  
Colon Carcinoma ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma
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