Pollen tube growth in the self-compatible sweet cherry genotype, ‘Cristobalina’, is slowed down after self-pollination

2013 ◽  
Vol 164 (1) ◽  
pp. 73-84 ◽  
Author(s):  
A.M. Cachi ◽  
A. Hedhly ◽  
J.I. Hormaza ◽  
A. Wünsch
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Sweet Cherry ◽  
The Self ◽  
Tube Growth ◽  
Self Pollination

scholarly journals Pollen tube growth and fruit set in quince (Cydonia oblonga Mill.)

2020 ◽  
Vol 18 (2) ◽  
pp. e0702
Author(s):  
Aleksandar Radovic ◽  
Radosav Cerovic ◽  
Dragan Milatovic ◽  
Dragan Nikolic
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Fruit Set ◽  
Pollen Tubes ◽  
The Self ◽  
Tube Growth ◽  
Open Pollination ◽  
Self Pollination ◽  
High Yields

Aim of study: To determine the self-compatibility level of eight quince cultivars.Area of study: The region of Belgrade (Central Serbia).Material and methods: Pollen tube growth in vivo and fruit set in two pollination variants (self- and open-pollination) were studied in eight quince cultivars. The quantitative parameters of pollen tube growth (average number of pollen tubes in the upper and middle third of the style, base of the style and in the ovary; the dynamics of pollen tube growth through these parts of the pistil) was determined using the fluorescence microscopy.Main results: The parameters of pollen tube growth and fruit set were primarily dependent on the genotype and variants of pollination. All studied parameters were significantly higher in the open-pollination variant compared with the self-pollination in all cultivars. In the self-pollination variant, ʻLeskovackaʼ and ʻVranjskaʼ had the highest number of pollen tubes that penetrated the ovary (2.10 and 0.54 in average, respectively), as well as the largest percentage of pistils with the penetration of pollen tubes in the nucellus of ovules six days after pollination (40.09% and 14.74%). Also, they had the highest percentage of initial fruit set (17.01% and 28.52%) and final fruit set (9.32% and 9.86%). Based on this, ʻLeskovackaʼ and ʻVranjskaʼ can be classified as self-compatible cultivars, while the others are self-incompatible.Research highlights: The majority of quince cultivars were self-incompatible. When establishing new orchards with these cultivars, care should be taken about the choice of pollenisers in order to achieve high yields.

The effect of genotype and temperature on pollen tube growth and fertilization in sweet cherry (Prunus avium L.)

Euphytica ◽  
2016 ◽  
Vol 209 (1) ◽  
pp. 121-136 ◽  
Author(s):  
Sanja Radičević ◽  
Radosav Cerović ◽  
Dragan Nikolić ◽  
Milena Đorđević
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Tube Growth ◽  
Prunus Avium L

scholarly journals Transcriptomic Analysis Reveals a Comprehensive Calcium- and Phytohormone-Dominated Signaling Response in Leymus chinensis Self-Incompatibility

2019 ◽  
Vol 20 (9) ◽  
pp. 2356 ◽  
Author(s):  
Shuangyan Chen ◽  
Junting Jia ◽  
Liqin Cheng ◽  
Pincang Zhao ◽  
Dongmei Qi ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Time Course ◽  
Molecular Mechanisms ◽  
Tube Growth ◽  
Leymus Chinensis ◽  
Self Incompatibility ◽  
Cross Pollination ◽  
Grass Family ◽  
Self Pollination

Sheepgrass (Leymus chinensis (Trin.) Tzvel.) is an economically and ecologically important forage in the grass family. Self-incompatibility (SI) limits its seed production due to the low seed-setting rate after self-pollination. However, investigations into the molecular mechanisms of sheepgrass SI are lacking. Therefore, microscopic observation of pollen germination and pollen tube growth, as well as transcriptomic analyses of pistils after self- and cross-pollination, were performed. The results indicated that pollen tube growth was rapidly inhibited from 10 to 30 min after self-pollination and subsequently stopped but preceded normally after cross-pollination. Time course comparative transcriptomics revealed different transcriptome dynamics between self- and cross-pollination. A pool of SI-related signaling genes and pathways was generated, including genes related to calcium (Ca2+) signaling, protein phosphorylation, plant hormone, reactive oxygen species (ROS), nitric oxide (NO), cytoskeleton, and programmed cell death (PCD). A putative SI response molecular model in sheepgrass was presented. The model shows that SI may trigger a comprehensive calcium- and phytohormone-dominated signaling cascade and activate PCD, which may explain the rapid inhibition of self-pollen tube growth as observed by cytological analyses. These results provided new insight into the molecular mechanisms of sheepgrass (grass family) SI.

Pollen‒pistil interaction in Lilium longiflorum : the role of the pistil in controlling pollen tube growth following cross- and self-pollinations

1982 ◽  
Vol 215 (1198) ◽  
pp. 45-62 ◽  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Active Component ◽  
Lilium Longiflorum ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
System A ◽  
Pollen Tube Elongation

By cytophysiological methods, the self-incompatibility mechanism of the breeding system in Lilium longiflorum has been examined with particular reference to the synthesis, location and nature of the stylar factors involved in the control of pollen tube development. A ‘bioassay’ has been developed by which the effect of stylar extracts on pollen tube elongation may be investigated. With use of this system, a crude fraction of proteins from the stylar fluid has been shown to inhibit pollen tube growth only when protein fractions from ‘self’ styles are used. The proteins of this fraction have been analysed by thin-layer gel electrofocusing. Changes in the profiles thus obtained following selfing and a heat treatment known to inactivate the self-incompatibility response indicate a highly polarized glycoprotein to be an active component of the system. The various ways by which such a glycoprotein could control pollen tube elongation are considered in detail, and these events in Lilium are discussed in the light of our knowledge of other self-incompatibility systems operating in angiosperms.

scholarly journals Compatibility Relationships in `Manzanillo' Olive

HortScience ◽  
1997 ◽  
Vol 32 (6) ◽  
pp. 1056-1058 ◽  
Author(s):  
Julián Cuevas ◽  
Vito S. Polito
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Olea Europaea ◽  
Fruit Set ◽  
Fold Increase ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Central California ◽  
Self Pollination ◽  
Olea Europaéa

We investigated pollination and fruit set parameters in `Manzanillo' olive (Olea europaea L.) following self-pollination and pollination with `Sevillano', `Ascolano', and `Mission' pollen. Results of analyses and experiments conducted over 2 years in central California indicated that `Manzanillo' behaves as a self-incompatible cultivar (index of self-incompatibility = 0.22 to 0.24). Pollination with `Sevillano' resulted in a more than 4-fold increase in fruit set over self-pollination. When `Mission' or `Ascolano' pollen was used, there was no increase over self-pollinated samples. Analyses of pollen tube growth, fertilization, initial fruit set, and final fruit set were consistent with `Manzanillo' being considered as a self-incompatible cultivar cross-incompatible with `Mission' and `Ascolano'. Our results indicate that `Manzanillo' is likely to be more productive when interplanted with `Sevillano' rather than when planted without a pollinizer or with `Mission' or `Ascolano'.

scholarly journals Reciprocal intercompatibility between Byrsonima Rich. exKunth species (Malpighiaceae), in an urban ecotonal fragment of Semi-Deciduous Seasonal Forest and Cerrado

2021 ◽  
pp. 211-221
Author(s):  
Frederic Mendes Hughes
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Prezygotic Isolation ◽  
Stigmatic Surface ◽  
Cross Pollination ◽  
Self Pollination ◽  
Isolation Mechanisms ◽  
Hypothesis Evaluation

Byrsonima species present floral monomorphism and sharing visitor/pollinator guild. These traits suggest the hypothesis evaluation of reciprocal inter-incompatibility between two syntopic Byrsonima species: B. pachyphylla and B. crassifolia. Pollen tube growth with similar behavior was observed in the stigmatic surface, pistil canal, ovary and micropylar channel to both species. In addition, partial self-incompatibility in self-pollination with greater fruiting in autogamy was observed. Cross-pollination and self-pollination coexist, and reciprocal intercompatibility occurs. Prezygotic isolation mechanisms are unlikely by the absence of abnormal pollen tubes, higher fruiting production and absence of hybrids in the study site. Las especies de Byrsonima presentan monomorfismo floral y comparten gremio de visitantes/polinizadores. Estos rasgos sugieren la evaluación de hipótesis de interincompatibilidad recíproca entre dos especies sintópicas de Byrsonima: B. pachyphylla y B. crassifolia. Se observó el crecimiento del tubo polínico con comportamiento similar en la superficie estigmática, canal del pistilo, ovario y canal micropilar de ambas especies. Además, se describió la autoincompatibilidad parcial en la autopolinización con mayor fructificación en la autogamia. La polinización cruzada y la autopolinización coexisten y se produce una intercompatibilidad recíproca. Los mecanismos de aislamiento precigóticos son improbables por la ausencia de tubos polínicos anormales, producción de frutos y ausencia de híbridos en la área de estudio.

scholarly journals Treatment of isolated pistils with protease inhibitors overcomes the self-incompatibility response in buckwheat

2007 ◽  
Vol 59 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Jovanka Miljus-Djukic ◽  
Svetlana Radovic ◽  
Vesna Maksimovic
Keyword(s):  
Pollen Tube ◽  
Protease Inhibitors ◽  
Pollen Tube Growth ◽  
Fluorescence Microscope ◽  
Fagopyrum Esculentum ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Fagopyrum Esculentum Moench ◽  
Pepstatin A

Isolated pistils of distylous buckwheat (Fagopyrum esculentum Moench) were treated with protease inhibitors (PMSF, pepstatin A, and antipain). Pistils were cross- or self- pollinated, and growth of pollen tubes was observed under a fluorescence microscope. Treatments with all inhibitors suppressed inhibition of self-pollen tube growth, suggesting that activity of proteases is involved in rejection of self-pollen during the SI response.

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