Expression profile of heat shock proteins in placental tissues of patients with preterm prelabor rupture of membranes and spontaneous preterm labor with intact membranes

2017 ◽  
Vol 78 (4) ◽  
pp. e12698 ◽  
Author(s):  
Lenka Dvorakova ◽  
Katarina Ivankova ◽  
Ladislav Krofta ◽  
Ilona Hromadnikova
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Expression Profile ◽  
Preterm Labor ◽  
Prelabor Rupture Of Membranes ◽  
Shock Proteins

scholarly journals Heat Shock Proteins and Heat Shock Protein-Antibody Complexes in Placental Tissues

1999 ◽  
Vol 7 (4) ◽  
pp. 180-185 ◽  
Author(s):  
M. Ziegert ◽  
S. S. Witkin ◽  
I. Sziller ◽  
H. Alexander ◽  
E. Brylla ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Pregnancy Outcome ◽  
Preterm Labor ◽  
Adverse Pregnancy Outcome ◽  
Increased Risk ◽  
Circulating Antibodies ◽  
Adverse Pregnancy ◽  
Shock Proteins

Objective:The relationship between pregnancy outcome and expression of the heat shock proteins (hsps) or hsp-antibody complexes of 60kD (hsp60), 70kD (hsp70), and 90kD (hsp90) in placental tissue and circulating antibodies to hsps was evaluated.Method:Expression of hsp60, hsp70, and hsp90 in placentae from 12 women with preterm birth, eight with intrauterine growth restriction (IUGR), and 10 with term birth, as well as the presence of the corresponding antibodies, was investigated by a new carbocyanine double fluorescence technique. Results were compared with microbiological findings and circulating antibodies to hsps in sera.Results:In each placental specimen examined, hsp60, hsp70, and hsp90 were identified. However, hsp70-antibody complexes were detected in only four of the preterm labor cases. Similarly, hsp60-antibody complexes were detected in only five preterm labor patients and in one patient with IUGR. None of the placentae contained hsp90-antibody complexes. In the preterm birth group, all patients with hsp60-antibody complexes were also positive for circulating antibodies to hsp60. The presence of hsp70-antibody complexes also correlated with hsp70 antibody in sera.Conclusions:Formation of hsp60- and hsp70-antibody complexes in the placenta may contribute to the induction of preterm birth. Women sensitized to these antibodies may be at increased risk for adverse pregnancy outcome. Infect. Dis. Obstet. Gynecol. 7:180–185, 1999.

scholarly journals Effect of melatonin supplementation on heat shock proteins expression profile in buffalo calves under summer stress

2021 ◽  
Vol 9 (1) ◽  
pp. 1197-1203
Author(s):  
Vijay D Domple ◽  
Amol R Padol ◽  
CD Malapure ◽  
Bhupesh P Kamdi ◽  
Roshan M Sarode
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Expression Profile ◽  
Buffalo Calves ◽  
Summer Stress ◽  
Shock Proteins

scholarly journals Dual Functions in Response to Heat Stress and Spermatogenesis: Characterization of Expression Profile of Small Heat Shock Proteins 9 and 10 in Goat Testis

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Wenjuan Xun ◽  
Liguang Shi ◽  
Ting Cao ◽  
Chunping Zhao ◽  
Ping Yu ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Expression Profile ◽  
High Temperature Stress ◽  
Small Heat Shock Proteins ◽  
Male Reproduction ◽  
Different Seasons ◽  
Hot Season ◽  
Shock Proteins ◽  
Environmental Temperatures

Small heat shock proteins 9 and 10 (HSPB9 and HSPB10) are two testis-specific expressed sHsps. The objective of this study was to investigate the mRNA expression profile of HSPB9 and HSPB10 in goat testis among the different seasons, ages, and environmental temperatures. Allocation of the two sHsps was also performed by immunohistochemistry. The results showed that the transcript levels of HSPB9 and HSPB10 were extremely high in the testis(P<0.01). The relative expression of HSBP9 and HSPB10 in testis showed a tendency to increase with age and then is maintained at the constant level after sexual maturity. HSPB9 and HSPB10 have significantly higher expression in the breeding season  (P<0.05)and hot season(P<0.01). Both HSPB9 and HSPB10 were found to be upregulated by high-temperature stress in testis(P<0.05), and the expressions of Hsp70 and Hsp90 were also increased simultaneously(P<0.01). Immunohistochemistry analysis localized HSPB9 expressed in spermatogonia, spermatocytes, and round spermatids and HSPB10 expressed in the elongate spermatids. In epididymis, strongly staining signal of HSPB10 was detected in pseudostratified columnar epithelium. In conclusion, the two testis-specific sHsps are closely related to male reproduction and heat tolerance. The results could provide valuable data for the further studies on HSPB9 and HSPB10.

Gene expression profile analysis of 4-phenylbutyrate treatment of IB3-1 bronchial epithelial cell line demonstrates a major influence on heat-shock proteins

2004 ◽  
Vol 16 (2) ◽  
pp. 204-211 ◽  
Author(s):  
Jerry M. Wright ◽  
Pamela L. Zeitlin ◽  
Liudmila Cebotaru ◽  
Sandra E. Guggino ◽  
William B. Guggino
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Cell Line ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Profile Analysis ◽  
Mrna Levels ◽  
Expression Profile Analysis ◽  
Shock Proteins

Most individuals with cystic fibrosis (CF) carry one or two mutations that result in a maturation defect of the full-length CFTR protein. The ΔF508 mutation results in a mutant protein that is degraded by the proteosome instead of progressing to the apical membrane where it functions as a cAMP-regulated chloride channel. 4-Phenylbutyrate (PBA) modulates heat-shock protein expression and promotes trafficking of ΔF508, thus permitting maturation and membrane insertion. The goal of this study was to gain insight into the genetic mechanism of PBA action through a large-scale analysis of gene expression. The Affymetrix genome-spanning U133 microarray set was used to compare mRNA expression levels in untreated IB3-1 cell line cultures with cultures treated with 1 mM PBA for 12 and 24 h. The most notable changes in mRNA levels were transient elevations in heat-shock proteins. The majority of genes downregulated throughout the application period were functionally associated with control of gene expression. Another set of genes increased in expression starting at 24 h, suggesting these are downstream effects of altered gene expression initiated by PBA. More than one-third of the genes in this late expressing set were identified as having potential significance in understanding the pathology of CF. Our results demonstrate the usefulness of gene expression profile analysis in understanding the consequences of PBA treatment and provide insights in how this drug exerts its effect on the trafficking of CFTR.

Heat shock proteins in willow (Salix viminalis)

1990 ◽  
Vol 80 (2) ◽  
pp. 301-306
Author(s):  
Tiina Vahala ◽  
Tage Eriksson ◽  
Peter Engstrom
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Salix Viminalis ◽  
Shock Proteins
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