Effect of melatonin supplementation on heat shock proteins expression profile in buffalo calves under summer stress

Small heat shock proteins 9 and 10 (HSPB9 and HSPB10) are two testis-specific expressed sHsps. The objective of this study was to investigate the mRNA expression profile of HSPB9 and HSPB10 in goat testis among the different seasons, ages, and environmental temperatures. Allocation of the two sHsps was also performed by immunohistochemistry. The results showed that the transcript levels of HSPB9 and HSPB10 were extremely high in the testis(P<0.01). The relative expression of HSBP9 and HSPB10 in testis showed a tendency to increase with age and then is maintained at the constant level after sexual maturity. HSPB9 and HSPB10 have significantly higher expression in the breeding season (P<0.05)and hot season(P<0.01). Both HSPB9 and HSPB10 were found to be upregulated by high-temperature stress in testis(P<0.05), and the expressions of Hsp70 and Hsp90 were also increased simultaneously(P<0.01). Immunohistochemistry analysis localized HSPB9 expressed in spermatogonia, spermatocytes, and round spermatids and HSPB10 expressed in the elongate spermatids. In epididymis, strongly staining signal of HSPB10 was detected in pseudostratified columnar epithelium. In conclusion, the two testis-specific sHsps are closely related to male reproduction and heat tolerance. The results could provide valuable data for the further studies on HSPB9 and HSPB10.

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A specific expression profile of heat-shock proteins and glucose-regulated proteins is associated with response to neoadjuvant chemotherapy in oesophageal adenocarcinomas

British Journal of Cancer ◽

10.1038/bjc.2013.319 ◽

2013 ◽

Vol 109 (2) ◽

pp. 370-378 ◽

Cited By ~ 16

Author(s):

J Slotta-Huspenina ◽

C Wolff ◽

E Drecoll ◽

M Feith ◽

M Bettstetter ◽

...

Keyword(s):

Heat Shock ◽

Neoadjuvant Chemotherapy ◽

Heat Shock Proteins ◽

Expression Profile ◽

Specific Expression ◽

Shock Proteins ◽

Response To Neoadjuvant Chemotherapy ◽

Glucose Regulated Proteins

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Expression profile of heat shock proteins in acute myeloid leukaemia patients reveals a distinct signature strongly associated with FLT3 mutation status - consequences and potentials for pharmacological intervention

British Journal of Haematology ◽

10.1111/j.1365-2141.2011.08960.x ◽

2011 ◽

Vol 156 (4) ◽

pp. 468-480 ◽

Cited By ~ 32

Author(s):

Håkon Reikvam ◽

Kimberley J. Hatfield ◽

Elisabeth Ersvaer ◽

Randi Hovland ◽

Jørn Skavland ◽

...

Keyword(s):

Heat Shock ◽

Heat Shock Proteins ◽

Acute Myeloid Leukaemia ◽

Myeloid Leukaemia ◽

Expression Profile ◽

Pharmacological Intervention ◽

Mutation Status ◽

Flt3 Mutation ◽

Shock Proteins ◽

Acute Myeloid

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Gene expression profile analysis of 4-phenylbutyrate treatment of IB3-1 bronchial epithelial cell line demonstrates a major influence on heat-shock proteins

Physiological Genomics ◽

10.1152/physiolgenomics.00160.2003 ◽

2004 ◽

Vol 16 (2) ◽

pp. 204-211 ◽

Cited By ~ 57

Author(s):

Jerry M. Wright ◽

Pamela L. Zeitlin ◽

Liudmila Cebotaru ◽

Sandra E. Guggino ◽

William B. Guggino

Keyword(s):

Gene Expression ◽

Heat Shock ◽

Heat Shock Proteins ◽

Cell Line ◽

Gene Expression Profile ◽

Expression Profile ◽

Profile Analysis ◽

Mrna Levels ◽

Expression Profile Analysis ◽

Shock Proteins

Most individuals with cystic fibrosis (CF) carry one or two mutations that result in a maturation defect of the full-length CFTR protein. The ΔF508 mutation results in a mutant protein that is degraded by the proteosome instead of progressing to the apical membrane where it functions as a cAMP-regulated chloride channel. 4-Phenylbutyrate (PBA) modulates heat-shock protein expression and promotes trafficking of ΔF508, thus permitting maturation and membrane insertion. The goal of this study was to gain insight into the genetic mechanism of PBA action through a large-scale analysis of gene expression. The Affymetrix genome-spanning U133 microarray set was used to compare mRNA expression levels in untreated IB3-1 cell line cultures with cultures treated with 1 mM PBA for 12 and 24 h. The most notable changes in mRNA levels were transient elevations in heat-shock proteins. The majority of genes downregulated throughout the application period were functionally associated with control of gene expression. Another set of genes increased in expression starting at 24 h, suggesting these are downstream effects of altered gene expression initiated by PBA. More than one-third of the genes in this late expressing set were identified as having potential significance in understanding the pathology of CF. Our results demonstrate the usefulness of gene expression profile analysis in understanding the consequences of PBA treatment and provide insights in how this drug exerts its effect on the trafficking of CFTR.

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