Effect of newborn bovine serum on cryopreservation of adult bovine testicular tissue

Andrologia ◽  
2013 ◽  
Vol 46 (3) ◽  
pp. 308-312 ◽  
Author(s):  
J. Y. Wu ◽  
Y. X. Sun ◽  
A. B. Wang ◽  
G. Y. Che ◽  
T. J. Hu ◽  
...  
Keyword(s):  
Bovine Serum ◽  
Testicular Tissue ◽  
Newborn Bovine Serum

Estimation of paracellular conductance of primary rat alveolar epithelial cell monolayers

2005 ◽  
Vol 98 (1) ◽  
pp. 138-143 ◽  
Author(s):  
Kwang-Jin Kim ◽  
Zea Borok ◽  
Carsten Ehrhardt ◽  
Brigham C. Willis ◽  
Claus-Michael Lehr ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Bovine Serum ◽  
Alveolar Epithelial Cell ◽  
Ringer Solution ◽  
Type I ◽  
Ion Conductance ◽  
Cell Monolayers ◽  
Alveolar Epithelial ◽  
Newborn Bovine Serum ◽  
Epithelial Cell Monolayers

Freshly isolated rat type II pneumocytes, when grown on permeable tissue culture-treated polycarbonate filters, form confluent alveolar epithelial cell monolayers (RAECM). Cells in RAECM undergo transdifferentiation, exhibiting over time morphological and phenotypic characteristics of type I pneumocytes in vivo. We recently reported that transforming growth factor-β1 (TGF-β1) decreases overall monolayer resistance ( Rte) and stimulates short-circuit current in a dose-dependent manner. In this study, we investigated the effects of TGF-β1 (50 pM) or 10% newborn bovine serum (NBS) on modulation of paracellular passive ion conductance and its contribution to total passive ion conductance across RAECM. On days 5–7 in culture, tight-junctional resistance ( Rtj, kΩcm2) of RAECM, cultured in minimally defined serum-free medium (MDSF) with or without TGF-β1 or NBS, was estimated from the relationship between observed transmonolayer voltage and resistance after addition of gramicidin D to apical potassium isethionate Ringer solution under open-circuit conditions. NaCl Ringer solution bathed the basolateral side throughout the experimental period. Results showed that transmonolayer conductance (1/ Rte) and tight-junctional conductance (1/ Rtj) are 0.59 and 0.14 mS/cm2 for control monolayers in MDSF, 1.59 and 0.38 mS/cm2 for monolayers exposed to TGF-β1, and 0.38 and 0.18 mS/cm2 for monolayers grown in the presence of NBS. The contributions to total transepithelial ion conductance by the paracellular pathway are estimated to be 23, 23, and 47% for control, TGF-β1-exposed, and newborn bovine serum (NBS)-treated RAECM, respectively.

scholarly journals Maintenance of bile acid synthesis and cholesterol 7α-hydroxylase activity in cultured rat hepatocytes

1990 ◽  
Vol 272 (1) ◽  
pp. 273-275 ◽  
Author(s):  
H M G Princen ◽  
P Meijer
Keyword(s):  
Bile Acid ◽  
Bovine Serum ◽  
Rat Hepatocytes ◽  
Acid Synthesis ◽  
Bile Acid Synthesis ◽  
Hydroxylase Activity ◽  
Newborn Bovine Serum ◽  
Cultured Rat Hepatocytes ◽  
Synthetic Capacity ◽  
Foetal Bovine Serum

Addition of foetal-bovine serum to rat hepatocytes cultured in Williams E medium resulted in improved maintenance of bile-acid-synthetic capacity and cholesterol 7 alpha-hydroxylase activity as compared with cultures supplemented with rat or newborn-bovine serum or cultures in a hormonally defined serum-free medium. Minimally, 5% (v/v) foetal-bovine serum was necessary to maintain these liver-specific functions. Serum factor(s) responsible for these effects were not dialysable or associated with lipoproteins, but were removed by charcoal extraction.

scholarly journals Acellularity of starfish embryonic mesenchyme cells as shown in vitro

Development ◽  
1990 ◽  
Vol 109 (1) ◽  
pp. 129-138
Author(s):  
H. Kaneko ◽  
S. Takaichi ◽  
M. Yamamoto ◽  
M. Dan-Sohkawa
Keyword(s):  
Extracellular Matrix ◽  
Bovine Serum ◽  
Sea Water ◽  
Three Dimensional ◽  
Newborn Bovine Serum ◽  
Cell Processes ◽  
Mesenchyme Cells ◽  
Network Pattern

Embryonic mesenchyme cells of the starfish are shown to be unexpectedly fusogenic in vitro. When archenteron complexes (archenterons and varying portions of the extracellular matrix {ecm} surrounding them) are isolated from starfish embryos and inoculated in sea water containing 4% newborn bovine serum, the mesenchyme cells form large syncytia on the substratum underneath each archenteron. These syncytia break into smaller fragments interconnected by fine cell processes within 24h. These networks have been studied morphologically, dynamically and ultrastructurally and found to lack cell borders between the constituent fragments. These fragments contain various numbers of nuclei ranging from 0 to 6 or more and move about constantly over the substratum, sometimes breaking into two and sometimes fusing with neighbouring fragments, so that the overall pattern of the network changes constantly. Our results also indicate that the network is three-dimensional i.e. it has crossing sites, the frequency of which seems to depend on the amount of the ECM excreted on the substratum. A similar network pattern is found among mesenchyme cells in vivo, which suggests that the features found in vitro reflect those in vivo.

Localization of Intracellular Antigens in thin Sections with Ferritin Labeled Antibody

1970 ◽  
Vol 28 ◽  
pp. 174-175
Author(s):  
M.S. Shahrabadi ◽  
T. Yamamoto
Keyword(s):  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
Antigenic Determinants ◽  
Conjugate Prior ◽  
Thin Sections ◽  
Specific Attachment ◽  
Intracellular Antigen ◽  
Antigen Antibody ◽  
Ideal Method ◽  
The Ideal

The technique of labeling of macromolecules with ferritin conjugated antibody has been successfully used for extracellular antigen by means of staining the specimen with conjugate prior to fixation and embedding. However, the ideal method to determine the location of intracellular antigen would be to do the antigen-antibody reaction in thin sections. This technique contains inherent problems such as the destruction of antigenic determinants during fixation or embedding and the non-specific attachment of conjugate to the embedding media. Certain embedding media such as polyampholytes (2) or cross-linked bovine serum albumin (3) have been introduced to overcome some of these problems.

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Albumin-Induced Endocytic Vacuoles in Tetrahymena Pyrijormis

1975 ◽  
Vol 33 ◽  
pp. 694-695
Author(s):  
L. J. Brenner ◽  
D. G. Osborne ◽  
B. L. Schumaker
Keyword(s):  
Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
Protein Concentration ◽  
Tetrahymena Pyriformis ◽  
Sequence Of Events ◽  
Cytoplasmic Bodies ◽  
Food Vacuoles ◽  
Mouse Ascites ◽  
Normal Human

Exposure of the ciliate, Tetrahymena pyriformis, strain WH6, to normal human or rabbit sera or mouse ascites fluids induces the formation of large cytoplasmic bodies. By electron microscopy these (LB) are observed to be membrane-bounded structures, generally spherical and varying in size (Fig. 1), which do not resemble the food vacuoles of cells grown in proteinaceous broth. The possibility exists that the large bodies represent endocytic vacuoles containing material concentrated from the highly nutritive proteins and lipoproteins of the sera or ascites fluids. Tetrahymena mixed with bovine serum albumin or ovalbumin solutions having about the same protein concentration (7g/100 ml) as serum form endocytic vacuoles which bear little resemblance to the serum-induced LB. The albumin-induced structures (Fig. 2) are irregular in shape, rarely spherical, and have contents which vary in density and consistency. In this paper an attempt is made to formulate the sequence of events which might occur in the formation of the albumin-induced vacuoles.

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