Epigallocatechin 3‐gallate improves the quality of bull semen cryopreservation

Effect of docosahexanoic acid on quality of frozen–thawed bull semen in BioXcell extender

Reproduction Fertility and Development ◽

10.1071/rd15089 ◽

2017 ◽

Vol 29 (3) ◽

pp. 490 ◽

Cited By ~ 3

Author(s):

Asmatullah Kaka ◽

Wahid Haron ◽

Rosnina Yusoff ◽

Nurhusien Yimer ◽

A. M. Khumran ◽

...

Keyword(s):

Lipid Peroxidation ◽

Liquid Nitrogen ◽

Sperm Motility ◽

Membrane Integrity ◽

Optimum Level ◽

Docosahexanoic Acid ◽

Normal Morphology ◽

Bull Semen ◽

Acrosome Integrity

This study was conducted to investigate the effect of docosahexanoic acid (DHA) supplementation in BioXcell extender on the quality of frozen–thawed bull semen. Twenty-four ejaculates were collected from three bulls (eight from each bull). Ejaculates with motility ≥70% and normal morphology ≥80% were extended into BioXcell extender to which 0 (control), 3, 5, 10 or 15 ng mL–1 DHA was added. The supplemented semen samples were incubated at 37°C for 15 min for DHA uptake by spermatozoa. Later, samples were cooled for 2 h at 5°C and packaged into 0.25-mL straws, frozen in liquid nitrogen for 24 h and subsequently thawed for evaluation. Results are presented as percentages ± s.e.m. Supplementation with DHA at 3 ng mL–1 significantly improved sperm functional parameters including sperm motility, normal morphology, viability, acrosome integrity and membrane integrity when compared with other supplemented groups and the control. Lipid peroxidation increased as the incorporation of DHA supplementation increased. In conclusion, 3 ng mL–1 concentration of DHA resulted in superior quality of frozen–thawed bull spermatozoa and is suggested as the optimum level of DHA to be added into BioXcell extender.

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Antioxidant effect of Mn2+ on capacitation and acrosome reaction of fresh and chilled cattle bull semen

Veterinary Science Development ◽

10.4081/vsd.2011.3479 ◽

2012 ◽

Vol 1 (1) ◽

pp. 18

Author(s):

Amrit Kaur Bansal ◽

Ranjna Sundhey Cheema ◽

Vinod Kumar Gandotra

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Acrosome Reaction ◽

Reactive Oxygen ◽

Antioxidant Effect ◽

Oxygen Species ◽

Sperm Capacitation ◽

Bull Semen

The aim of this paper was to investigate the antioxidant effect of Mn2+ (200 mM) on the sperm capacitation and acrosome reaction of fresh and chilled cattle bull semen. It has been found that Mn2+ supplementation improves (P≤0.05) the motility at 0, 2, 4 and 6 h of incubation. MDA (malondialdehyde), end product of lipid peroxidation, decreases significantly (P≤0.05) with the supplementation of manganese at 0- and 6-hr of incubation both in fresh and chilled semen. Manganese also increases acrosome reaction significantly (P≤0.05) both in fresh and chilled semen at 0, 4 and 6 h of incubation. Therefore, our findings suggest the role of Mn2+supplementation in improving the quality of cattle bull semen by its scavenging property<em> i.e.</em> reduction in the production of reactive oxygen species during its storage at 4°C or incubation at 37°C for capacitation.

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Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

Animal Bioscience ◽

10.5713/ajas.20.0025 ◽

2021 ◽

Vol 34 (2) ◽

pp. 198-204 ◽

Cited By ~ 1

Author(s):

Suherni Susilowati ◽

Trilas Sardjito ◽

Imam Mustofa ◽

Oky Setio Widodo ◽

Rochmah Kurnijasanti

Keyword(s):

Pregnancy Rate ◽

Dna Fragmentation ◽

Green Tea ◽

Prostaglandin F2α ◽

Green Tea Extract ◽

Control Group ◽

Bull Semen ◽

Frozen Semen ◽

Tea Extract

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

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Bacteriospermia and Sperm Quality of Cryopreserved Bull Semen Used in Artificial Insemination of Cows in South Wollo Zone, Ethiopia

Veterinary Medicine International ◽

10.1155/2020/2098315 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Abadi Amare Reda ◽

Gizat Almaw ◽

Solomon Abreha ◽

Wedajo Tadeg ◽

Belege Tadesse

Keyword(s):

Bacterial Contamination ◽

Semen Quality ◽

Sperm Quality ◽

Bacterial Load ◽

Bacterial Count ◽

Normal Morphology ◽

Bull Semen ◽

Significant Difference ◽

Processing And Storage

The objectives of this trial were to estimate prevalence of bacteriospermia, to determine the bacterial load, and to isolate the types of bacteria as well as to assess the association between bacterial load and sperm quality traits in cryopreserved bull semen in field conditions in the South Wollo Zone. A total of 309 cryopreserved straws of semen from the Holstein Friesian (HF)-cross bull (n = 180 straws) and pure Jersey bull (n = 129 straws) were investigated. Bacteriological assessments of the presence of aerobic bacteria, estimation of bacterial count and bacterial isolation, as well as semen quality were performed. Aerobic bacterial contamination was prevalent in 38.8% of the semen straws. No significant difference in the prevalence of bacteriospermia was observed among bulls although the HF-cross bull had a higher prevalence (40.0%). But, significant difference in prevalence of bacteriospermia was found among semen ejaculates of the same bull. The risk of bacteriospermia in the HF-cross bull was higher (Odds ratio = 1.86, 95% CI = 0.168–20.26) compared to Jersey although not significant. Overall average bacterial load of 50.38 ± 16.29 colony-forming units (CFU)/ml (from nil to 1318.20 CFU/ml) was found. No significant difference in bacterial count among bulls and their ejaculates was observed. Moreover, correlation analysis revealed that the proportions of motility, live, and normal morphology were negatively influenced by an increase in the bacterial contamination of semen. In this study, three isolates of coagualse-negative Staphylococcus species and one isolate of Corynebacterium species were found. Average percentages of sperm motility (48.35 ± 1.23), live (66.08 ± 1.0), and normal morphology (80.62 ± 1.24) were observed. It was concluded that cryopreservation does not guarantee the quality of semen from bacterial contamination. Hence, meticulous care should be adopted to prevent contamination of semen by bacteria during collection, transportation, processing, and storage times.

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84 EVALUATION OF BULL SEMEN AT 1 H VS. 48 H OF POST-FROZEN STABILIZATION TIME

Reproduction Fertility and Development ◽

10.1071/rdv18n2ab84 ◽

2006 ◽

Vol 18 (2) ◽

pp. 150

Author(s):

G. M. Brogliatti ◽

G. Larraburu ◽

R. Cavia ◽

M. E. Carini

Keyword(s):

Liquid Nitrogen ◽

Artificial Insemination ◽

Semen Analysis ◽

Computer Assisted ◽

Stabilization Time ◽

Bull Semen ◽

Straight Line ◽

Semen Extender ◽

Lateral Head

The process of cryopreservation of bull semen in liquid nitrogen at −196°C is usually carried out after 3 to 6 h of refrigeration at 4°C post-collection. To guarantee the quality of the final product, the frozen straws are evaluated after cryopreservation. The seminal samples are usually stabilized during 48 h before being analyzed (Hafez, Reproduction and Artificial Insemination in Animals, 1989); this would retard the possible commercialization. The objective of the present study was to determine motility parameters and viability of semen doses stabilized by 1 h or more than 48 h in liquid nitrogen at −196°C. A total of 122 ejaculated from 23 different adult bulls (Angus, Brangus, Braford, and Hereford) were evaluated in an artificial insemination center between January and April 2005. The semen was diluted in a semi-defined semen extender (Andromed, Minitub, Germany) and frozen in an automatic freezer (Digicool, IMV, France). Parameters of velocity average path (VAP, μm/s), velocity straight line (VSL, µm/s), amplitude lateral head (ALH, µm), linearity (LIN, %), percentage of rapid cells (RAPID, %), and viability (VIA, %) were determined by Computer Assisted Semen Analysis (CASA, HTM-ceros 12.1, Berkeley, CA, USA). The obtained results were analyzed statistically with T Student and are summarized in Table 1. The results indicate that there is no difference in the velocity of the spermatozoa evaluated 1 h or 48 h post-frozen. There is no difference in VAP, VSL, movement of amplitude lateral head (ALH), or linearity (LIN). The percentage of viable spermatozoa was not affected in either group. Statistical analysis indicates that there is no difference (P > 0.05) in any of the evaluated parameters. The results demonstrate that spermatic motility and viability of frozen bull semen could be evaluated before 48 h post-frozen. This allows reduction of the time between freezing and evaluation and immediate availability of the bull straws. Table 1. Parameters of motility and viability at 1 h vs. 48 h of post-frozen stabilization time This research was supported by Centro Genético Bovino EOLIA S.A.

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Supplementation of Arabian jujube (Ziziphus spina Christi) leaf extracts as extender material on the quality of Bali bull semen

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/492/1/012079 ◽

2020 ◽

Vol 492 ◽

pp. 012079

Author(s):

Sahiruddin ◽

Widjiati ◽

A L Toleng ◽

M Yusuf ◽

Masturi

Keyword(s):

Leaf Extracts ◽

Bull Semen

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Fructose content and fructolysis in semen. Practical application in the evaluation of semen quality

The Journal of Agricultural Science ◽

10.1017/s0021859600006109 ◽

1948 ◽

Vol 38 (3) ◽

pp. 323-331 ◽

Cited By ~ 109

Author(s):

T. Mann

Keyword(s):

Semen Quality ◽

Poor Quality ◽

Small Samples ◽

Bull Semen ◽

Accessory Glands ◽

Reduced Rate ◽

High Level ◽

Fresh Semen

SUMMARY1. A method is described whereby fructose content and fructolysis can be assayed accurately in small samples of semen. The advantages of this method lie in its simplicity, accuracy and practical convenience as a tool for the assessment of semen quality, applicable also under field conditions.2. The content of fructose in fresh semen depends upon the secretory function of accessory glands which is influenced directly by the activity of the male sex hormone. A low level of seminal fructose may coincide with other symptoms of hormonal malfunction and poor quality of spermatozoa. A high level of seminal fructose indicates satisfactory functional ability of the accessory glands, but it does not necessarily coincide with high quality of spermatozoa as expressed in terms of density and motility.3. The normal level of fructose in fresh semen undergoes frequent fluctuations which can be observed if semen collections are made from the same individual at different times. Considerable variations in the sperm/fructose ratio may also occur in the semen of the same individual as illustrated by the results of an ‘exhaustion test’.4. Fructose disappears from semen incubated in vitro. The rate of fructose disappearance forms a convenient measure of sperm fructolysis. The normal rate of fructolysis in bull semen is 1·4–2 mg. fructose per 109 sperm cells in 1 hr. at 37° C. At this high level it can be maintained until almost the whole reserve of fructose has been exhausted. Azoospermic and necrospennic semen, as well as that from vasectomized animals, are unable to utilize fructose. A reduced rate of fructolysis is found in low quality semen of subfertile and infertile animals.5. The conditions of sperm survival in semen incubated in narrow tubes as used for the fructolysis test as well as for storage of semen in the practice of artificial insemination, are almost purely anaerobic. Under such conditions the survival of spermatozoa must largely depend upon fructolysis and not upon respiration.

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