scholarly journals Contextualizing the biological relevance of standardized high‐resolution respirometry to assess mitochondrial function in permeabilized human skeletal muscle

2021 ◽  
Author(s):  
Robert A. Jacobs ◽  
Carsten Lundby
Keyword(s):  
Skeletal Muscle ◽  
High Resolution ◽  
Mitochondrial Function ◽  
Human Skeletal Muscle ◽  
Biological Relevance

1891-P: Palmitate-Enriched Lipid Ingestion Acutely Induces Insulin Resistance Associated with PKCγ Activation and Impaired Mitochondrial Function in Human Skeletal Muscle

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 1891-P
Author(s):  
THERESIA SARABHAI ◽  
CHRYSI KOLIAKI ◽  
SABINE KAHL ◽  
DOMINIK PESTA ◽  
LUCIA MASTROTOTARO ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Mitochondrial Function ◽  
Human Skeletal Muscle

scholarly journals Resistance Exercise Training Alters Mitochondrial Function in Human Skeletal Muscle

2015 ◽  
Vol 47 (9) ◽  
pp. 1922-1931 ◽  
Author(s):  
CRAIG PORTER ◽  
PAUL T. REIDY ◽  
NISHA BHATTARAI ◽  
LABROS S. SIDOSSIS ◽  
BLAKE B. RASMUSSEN
Keyword(s):  
Skeletal Muscle ◽  
Exercise Training ◽  
Resistance Exercise ◽  
Mitochondrial Function ◽  
Human Skeletal Muscle ◽  
Resistance Exercise Training

scholarly journals The C57Bl/6 mouse serves as a suitable model of human skeletal muscle mitochondrial function

2013 ◽  
Vol 98 (4) ◽  
pp. 908-921 ◽  
Author(s):  
Robert A. Jacobs ◽  
Víctor Díaz ◽  
Anne-Kristine Meinild ◽  
Max Gassmann ◽  
Carsten Lundby
Keyword(s):  
Skeletal Muscle ◽  
Mitochondrial Function ◽  
Human Skeletal Muscle ◽  
Suitable Model

Ethylmalonic encephalopathy ETHE1 p. D165H mutation alters the mitochondrial function in human skeletal muscle proteome

Mitochondrion ◽  
2021 ◽  
Author(s):  
Gajanan Sathe ◽  
Sekar Deepha ◽  
Narayanappa Gayathri ◽  
Madhu Nagappa ◽  
Bindu Parayil Sankaran ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mitochondrial Function ◽  
Human Skeletal Muscle ◽  
Ethylmalonic Encephalopathy ◽  
Muscle Proteome

Mitochondrial function in human skeletal muscle is not impaired by high intensity exercise

1999 ◽  
Vol 437 (4) ◽  
pp. 562-568 ◽  
Author(s):  
M. Tonkonogi ◽  
B. Walsh ◽  
T. Tiivel ◽  
V. Saks ◽  
K. Sahlin
Keyword(s):  
Skeletal Muscle ◽  
Mitochondrial Function ◽  
High Intensity ◽  
Human Skeletal Muscle ◽  
High Intensity Exercise

294-LB: Transcriptome-Wide Signals Converge Upon Mitochondrial Function in Developmental Stages of Type 2 Diabetes in Human Skeletal Muscle

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 294-LB
Author(s):  
LUKASZ SZCZERBINSKI ◽  
MAGDALENA NIEMIRA ◽  
KAROL SZCZERBINSKI ◽  
URSZULA PUCHTA ◽  
ELWIRA SIEWIEC ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Skeletal Muscle ◽  
Mitochondrial Function ◽  
Human Skeletal Muscle ◽  
Developmental Stages

scholarly journals Daily heat treatment maintains mitochondrial function and attenuates atrophy in human skeletal muscle subjected to immobilization

2019 ◽  
Vol 127 (1) ◽  
pp. 47-57 ◽  
Author(s):  
Paul S. Hafen ◽  
Kaitlin Abbott ◽  
Jennifer Bowden ◽  
Ryan Lopiano ◽  
Chad R. Hancock ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Skeletal Muscle ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Mitochondrial Function ◽  
Human Skeletal Muscle ◽  
Respiratory Capacity ◽  
Sham Treatment ◽  
Limb Immobilization

Skeletal muscle immobilization leads to atrophy, decreased metabolic health, and substantial losses in function. Animal models suggest that heat stress can provide protection against atrophy in skeletal muscle. This study investigated the effects of daily heat therapy on human skeletal muscle subjected to 10 days of immobilization. Muscle biopsies were collected, and MRIs were analyzed from the vastus lateralis of 23 healthy volunteers (11 women, 12 men) before and after either 10 days of immobilization with a daily sham treatment (Imm) or with a targeted, daily 2-h heat treatment using pulsed shortwave diathermy (Imm + H). Diathermy increased intramuscular temperature 4.2 ± 0.29°C ( P < 0.0001), with no change during sham treatment. As a result, heat shock protein (HSP)70 and HSP90 increased ( P < 0.05) following Imm + H (25 ± 6.6 and 20 ± 7.4%, respectively) but were unaltered with Imm only. Heat treatment prevented the immobilization-induced loss of coupled (−27 ± 5.2% vs. −8 ± 6.0%, P = 0.0041) and uncoupled (−25 ± 7.0% vs. −10 ± 3.9%, P = 0.0302) myofiber respiratory capacity. Likewise, heat treatment prevented the immobilization-induced loss of proteins associated with all five mitochondrial respiratory complexes ( P < 0.05). Furthermore, decreases in muscle cross-sectional area following Imm were greater than Imm + H at both the level of the whole muscle (−7.6 ± 0.96% vs. −4.5 ± 1.09%, P = 0.0374) and myofiber (−10.8 ± 1.52% vs. −5.8 ± 1.49%, P = 0.0322). Our findings demonstrate that daily heat treatments, applied during 10 days of immobilization, prevent the loss of mitochondrial function and attenuate atrophy in human skeletal muscle. NEW & NOTEWORTHY Limb immobilization results in substantial decreases in skeletal muscle size, function, and metabolic capacity. To date, there are few, if any, interventions to prevent the deleterious effects of limb immobilization on skeletal muscle health. Heat stress has been shown to elicit a stress response, resulting in increased heat shock protein expression and improved mitochondrial function. We show that during 10 days of lower-limb immobilization in humans, daily exposure to heat stress maintains mitochondrial respiratory capacity and attenuates atrophy in skeletal muscle. Our findings suggest that heat stress may serve as an effective therapeutic strategy to attenuate the decreases of muscle mass and metabolic function that accompany periods of disuse.

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