The in vitro properties of dermal papilla cell lines established from human hair follicles

1986 ◽  
Vol 114 (4) ◽  
pp. 425-430 ◽  
Author(s):  
A.G. MESSENGER ◽  
H. JENNIFER ◽  
S.S. BLEEHEN
Keyword(s):  
Cell Lines ◽  
Human Hair ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cell

scholarly journals Establishment of Rat Dermal Papilla Cell Lines that Sustain the Potency to Induce Hair Follicles from Afollicular Skin

1998 ◽  
Vol 111 (5) ◽  
pp. 767-775 ◽  
Author(s):  
Mutsumi Inamatsu ◽  
Takashi Matsuzaki ◽  
Hiroko Iwanari ◽  
Katsutoshi Yoshizato
Keyword(s):  
Cell Lines ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cell

scholarly journals Towards developing an organotypic model for the preclinical study and manipulation of human hair matrix-dermal papilla interactions

2021 ◽  
Author(s):  
Christopher I. Platt ◽  
Jeremy Chéret ◽  
Ralf Paus
Keyword(s):  
Human Hair ◽  
Ex Vivo ◽  
Dermal Papilla ◽  
Mesenchymal Cell ◽  
Preclinical Study ◽  
In Vitro Models ◽  
Hair Follicles ◽  
Hair Matrix ◽  
Anagen Hair

AbstractOrgan culture of microdissected scalp hair follicles (HFs) has become the gold standard for human ex vivo hair research; however, availability is becoming very limited. Although various simplistic “HF-equivalent” in vitro models have been developed to overcome this limitation, they often fail to sufficiently mimic the complex cell–cell and cell–matrix interactions between epithelial and mesenchymal cell populations that underlie the specific growth processes occurring in a native HF. Here, we have attempted to overcome these limitations by developing a novel human hair research model that combines dermal papilla (DP) fibroblasts, cultured as 3-dimensional (3D) spheroids (DPS), with plucked anagen hair shafts (HS). We show that DPS express HF inductivity markers, such as alkaline phosphatase (ALP), versican and noggin, while plucked HSs retain substantial remnants of the anagen hair matrix. When cultured together, DPS adhere to and surround the plucked HS (HS-DPS), and significantly enhance HS expression of the differentiation marker keratin-85 (K85; p < 0.0001), while simultaneously decreasing the percentage of TUNEL + cells in the proximal HS (p = 0.0508). This simple model may offer a physiologically relevant first step toward evaluating HF differentiation in the human anagen hair matrix.

scholarly journals Herbal Extracts Induce Dermal Papilla Cell Proliferation of Human Hair Follicles

2015 ◽  
Vol 27 (6) ◽  
pp. 667 ◽  
Author(s):  
Hosein Rastegar ◽  
Hamidreza Ahmadi Ashtiani ◽  
Mahmoud Aghaei ◽  
Behrooz Barikbin ◽  
Amirohushang Ehsani
Keyword(s):  
Cell Proliferation ◽  
Human Hair ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Herbal Extracts ◽  
Dermal Papilla Cell

scholarly journals Comparison of Effects on Gene Expression Activity of Low-Molecular-Weight Lychee Fruit Polyphenol (Oligonol®), Adenosine, and Minoxidil in Human Dermal Papilla Cells

2017 ◽  
Vol 3 (1) ◽  
pp. 5 ◽  
Author(s):  
Koji Wakame ◽  
Akifumi Nakata ◽  
Keisuke Sato ◽  
Yoshihiro Mihara ◽  
Jun Takanari ◽  
...  
Keyword(s):  
Cell Lines ◽  
Dna Microarrays ◽  
Food Product ◽  
Dermal Papilla ◽  
Pcr Analysis ◽  
Low Molecular Weight ◽  
Dermal Papilla Cells ◽  
Dermal Papilla Cell ◽  
Expression Of Genes ◽  
Expression Activity

Background: Oligonol® (OLG) is a functional food product and ingredient for cosmetics derived from a lychee fruit polyphenol. It has been reported to act on the skin as an anti-inflammatory and prevent UVB-induced skin damage.Aim: In this study, with the aim of exploring new functionalities of OLG on the scalp, we investigated the effect of OLG on human dermal papilla cells by comparing with adenosine and minoxidil at the genetic level.Method: OLG, adenosine, and minoxidil were applied to human dermal papilla cell lines for 24 h, after which VEGF, FGF-7, WNT5a, and WNT10a mRNA expressions were measured by real-time PCR analysis. Additionally, using DNA microarrays, we investigated the effect on 205 inflammation-related genes.Result: Consequently, in human dermal papilla cell lines, FGF-7 and WNT10a mRNA expression were observed in 100 µg/mL OLG-supplemented cells. The results of the DNA microarray analysis showed that 10 genes were suppressed by OLG.Conclusions: OLG may be expected to affect function of human dermal papilla cell by regulating the expression of genes related to cell proliferation and inflammation.

Smooth muscle alpha-actin is a marker for hair follicle dermis in vivo and in vitro

1991 ◽  
Vol 99 (3) ◽  
pp. 627-636 ◽  
Author(s):  
C.A. Jahoda ◽  
A.J. Reynolds ◽  
C. Chaponnier ◽  
J.C. Forester ◽  
G. Gabbiani
Keyword(s):  
Smooth Muscle ◽  
Hair Follicle ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells ◽  
Smooth Muscle Alpha Actin ◽  
Actin Expression ◽  
Alpha Actin ◽  
Sheath Cells

We have examined the expression of smooth muscle alpha-actin in hair follicles in situ, and in hair follicle dermal cells in culture by means of immunohistochemistry. Smooth muscle alpha-actin was present in the dermal sheath component of rat vibrissa, rat pelage and human follicles. Dermal papilla cells within all types of follicles did not express the antigen. However, in culture a large percentage of both hair dermal papilla and dermal sheath cells were stained by this antibody. The same cells were negative when tested with an antibody to desmin. Overall, explant-derived skin fibroblasts had relatively low numbers of positively marked cells, but those from skin regions of high hair-follicle density displayed more smooth muscle alpha-actin expression than fibroblasts from areas with fewer follicles. 2-D SDS-PAGE confirmed that, unlike fibroblasts, cultured papilla cells contained significant quantities of the alpha-actin isoform. The rapid switching on of smooth muscle alpha-actin expression by dermal papilla cells in early culture, contrasts with the behaviour of smooth muscle cells in vitro, and has implications for control of expression of the antigen in normal adult systems. The very high percentage of positively marked cultured papilla and sheath cells also provides a novel marker of cells from follicle dermis, and reinforces the idea that they represent a specialized cell population, contributing to the heterogeneity of fibroblast cell types in the skin dermis, and possibly acting as a source of myofibroblasts during wound healing.

Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines retaining a differentiated phenotype

1994 ◽  
Vol 107 (7) ◽  
pp. 1761-1772
Author(s):  
W. Filsell ◽  
J.C. Little ◽  
A.J. Stones ◽  
S.P. Granger ◽  
S.A. Bayley
Keyword(s):  
Cell Lines ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Growth Cycle ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Early Passage ◽  
Gene Encoding ◽  
Dermal Papilla Cells ◽  
Dermal Papilla Cell

The dermal papilla is a discrete group of cells at the base of the hair follicle and is implicated in controlling the hair growth cycle. Early passage dermal papilla cells can induce hair growth in vivo, but, upon further culturing, this property is lost. In order to study the events occurring in hair induction, a representative dermal papilla cell line was required. We have transfected passage 1 rat vibrissa dermal papilla cells with a polyomavirus large T gene encoding a temperature-sensitive T antigen, and generated permanent cell lines in which the immortalizing function can be switched off by temperature shift. The cells established without crisis, resembled cells in the starting population, and retained the aggregative properties of early passage dermal papilla cells. Growth studies were performed on the immortalized cell lines, which showed that transferring the cells to the restrictive temperature for the large T gene product resulted in cell senescence or quiescence, and changes in morphology. Implantation of cell pellets into the ears of immunologically compatible rats showed that the immortal cells retained hair-inductive ability. Cytokines are believed to have an important role in the control of hair growth. The pattern of cytokine gene expression in the immortal cell lines was compared with early passage dermal papilla cells and a non-hair-inducing dermal papilla cell line, using reverse transcriptase-polymerase chain reaction. Epidermal growth factor, tumour necrosis factor, and interleukin-1a were detected in the immortalized and non-hair-inducing dermal papilla cell lines, but were absent in passage 2 dermal papilla cells. All other cytokines examined were detected in all the cell types under study. These results demonstrate that the polyomavirus large Ttsa-immortalized dermal papilla cell lines are very similar to passage 2 dermal papilla cells and thus provide a good model for hair growth studies. Cytokine expression profiles indicate that the expression of several cytokines may be implicated in hair induction. Further studies are under way to investigate the relationship between cytokine expression and the hair growth cycle.

scholarly journals The local hypothalamic–pituitary–adrenal axis in cultured human dermal papilla cells

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Eun Young Lee ◽  
You Jin Nam ◽  
Sangjin Kang ◽  
Eun Ju Choi ◽  
Inbo Han ◽  
...  
Keyword(s):  
Hpa Axis ◽  
Hair Loss ◽  
Human Hair ◽  
Stress Hormones ◽  
Dermal Papilla ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Follicle Cells ◽  
Dermal Papilla Cells ◽  
Underlying Mechanisms

Abstract Background Stress is an important cause of skin disease, including hair loss. The hormonal response to stress is due to the HPA axis, which comprises hormones such as corticotropin releasing factor (CRF), adrenocorticotropic hormone (ACTH), and cortisol. Many reports have shown that CRF, a crucial stress hormone, inhibits hair growth and induces hair loss. However, the underlying mechanisms are still unclear. The aim of this study was to examine the effect of CRF on human dermal papilla cells (DPCs) as well as hair follicles and to investigate whether the HPA axis was established in cultured human DPCs. Results CRF inhibited hair shaft elongation and induced early catagen transition in human hair follicles. Hair follicle cells, both human DPCs and human ORSCs, expressed CRF and its receptors and responded to CRF. CRF inhibited the proliferation of human DPCs through cell cycle arrest at G2/M phase and induced the accumulation of reactive oxygen species (ROS). Anagen-related cytokine levels were downregulated in CRF-treated human DPCs. Interestingly, increases in proopiomelanocortin (POMC), ACTH, and cortisol were induced by CRF in human DPCs, and antagonists for the CRF receptor blocked the effects of this hormone. Conclusion The results of this study showed that stress can cause hair loss by acting through stress hormones. Additionally, these results suggested that a fully functional HPA axis exists in human DPCs and that CRF directly affects human DPCs as well as human hair follicles under stress conditions.

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