scholarly journals Comparison of Effects on Gene Expression Activity of Low-Molecular-Weight Lychee Fruit Polyphenol (Oligonol®), Adenosine, and Minoxidil in Human Dermal Papilla Cells

2017 ◽  
Vol 3 (1) ◽  
pp. 5 ◽  
Author(s):  
Koji Wakame ◽  
Akifumi Nakata ◽  
Keisuke Sato ◽  
Yoshihiro Mihara ◽  
Jun Takanari ◽  
...  
Keyword(s):  
Cell Lines ◽  
Dna Microarrays ◽  
Food Product ◽  
Dermal Papilla ◽  
Pcr Analysis ◽  
Low Molecular Weight ◽  
Dermal Papilla Cells ◽  
Dermal Papilla Cell ◽  
Expression Of Genes ◽  
Expression Activity

Background: Oligonol® (OLG) is a functional food product and ingredient for cosmetics derived from a lychee fruit polyphenol. It has been reported to act on the skin as an anti-inflammatory and prevent UVB-induced skin damage.Aim: In this study, with the aim of exploring new functionalities of OLG on the scalp, we investigated the effect of OLG on human dermal papilla cells by comparing with adenosine and minoxidil at the genetic level.Method: OLG, adenosine, and minoxidil were applied to human dermal papilla cell lines for 24 h, after which VEGF, FGF-7, WNT5a, and WNT10a mRNA expressions were measured by real-time PCR analysis. Additionally, using DNA microarrays, we investigated the effect on 205 inflammation-related genes.Result: Consequently, in human dermal papilla cell lines, FGF-7 and WNT10a mRNA expression were observed in 100 µg/mL OLG-supplemented cells. The results of the DNA microarray analysis showed that 10 genes were suppressed by OLG.Conclusions: OLG may be expected to affect function of human dermal papilla cell by regulating the expression of genes related to cell proliferation and inflammation.

Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines retaining a differentiated phenotype

1994 ◽  
Vol 107 (7) ◽  
pp. 1761-1772
Author(s):  
W. Filsell ◽  
J.C. Little ◽  
A.J. Stones ◽  
S.P. Granger ◽  
S.A. Bayley
Keyword(s):  
Cell Lines ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Growth Cycle ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Early Passage ◽  
Gene Encoding ◽  
Dermal Papilla Cells ◽  
Dermal Papilla Cell

The dermal papilla is a discrete group of cells at the base of the hair follicle and is implicated in controlling the hair growth cycle. Early passage dermal papilla cells can induce hair growth in vivo, but, upon further culturing, this property is lost. In order to study the events occurring in hair induction, a representative dermal papilla cell line was required. We have transfected passage 1 rat vibrissa dermal papilla cells with a polyomavirus large T gene encoding a temperature-sensitive T antigen, and generated permanent cell lines in which the immortalizing function can be switched off by temperature shift. The cells established without crisis, resembled cells in the starting population, and retained the aggregative properties of early passage dermal papilla cells. Growth studies were performed on the immortalized cell lines, which showed that transferring the cells to the restrictive temperature for the large T gene product resulted in cell senescence or quiescence, and changes in morphology. Implantation of cell pellets into the ears of immunologically compatible rats showed that the immortal cells retained hair-inductive ability. Cytokines are believed to have an important role in the control of hair growth. The pattern of cytokine gene expression in the immortal cell lines was compared with early passage dermal papilla cells and a non-hair-inducing dermal papilla cell line, using reverse transcriptase-polymerase chain reaction. Epidermal growth factor, tumour necrosis factor, and interleukin-1a were detected in the immortalized and non-hair-inducing dermal papilla cell lines, but were absent in passage 2 dermal papilla cells. All other cytokines examined were detected in all the cell types under study. These results demonstrate that the polyomavirus large Ttsa-immortalized dermal papilla cell lines are very similar to passage 2 dermal papilla cells and thus provide a good model for hair growth studies. Cytokine expression profiles indicate that the expression of several cytokines may be implicated in hair induction. Further studies are under way to investigate the relationship between cytokine expression and the hair growth cycle.

Establishment and Functional Characterization of Immortalized Rabbit Dermal Papilla Cell Lines

2021 ◽  
Author(s):  
Jiali Li ◽  
Bohao Zhao ◽  
Chen Zhang ◽  
Xiyu Zhang ◽  
Yingying Dai ◽  
...  
Keyword(s):  
Cell Lines ◽  
Growth And Development ◽  
Functional Characterization ◽  
Dermal Papilla ◽  
Early Passage ◽  
Dermal Papilla Cells ◽  
Viability Analysis ◽  
Periodic Growth ◽  
Basic Characteristics ◽  
Immortalized Cell

Abstract Background Hair follicle (HF) undergo periodic growth and development in mammals, which regulated by dermal papilla cells (DPCs) are reported to play an important role in the HF morphogenesis and development. However, primary DPCs have low proliferative activity, age quickly, and fresh cell isolation is both time-consuming and laborious. Method In this study, we introduced SV40LT into dissociated early passage rabbit vibrissae DPCs with lentiviral vectors and established seven immortalized DP cell lines (R-1, R-2, R-3, R-4, R-5, R-6 and R-7). Result These cell lines displayed early passage morphology and displayed high alkaline phosphatase activity. RT-PCR and immunofluorescence staining showed that all the immortalized cell lines expressed the DPC markers (α-SMA ,IGF1, ALPL, FGF2, BMP2 and TGFβ2; α-SMA and VIM protein), but α-SMA was only expressed well in R-3, R-4, and R-7. Furthermore, it was found that R-7 was the only line to survive beyond 50 passages. Compared to melanoma cells, R-7 did not undergo malignant transformation. Karyotyping and cell growth viability analysis illustrated that the R-7 cell line preserved the basic characteristics of primary DPCs. Conclusion The R-7 DPCs established have potential application for future hair research. The study provides the theoretical basis in the cell research of HF growth and development.

scholarly journals Establishment of Rat Dermal Papilla Cell Lines that Sustain the Potency to Induce Hair Follicles from Afollicular Skin

1998 ◽  
Vol 111 (5) ◽  
pp. 767-775 ◽  
Author(s):  
Mutsumi Inamatsu ◽  
Takashi Matsuzaki ◽  
Hiroko Iwanari ◽  
Katsutoshi Yoshizato
Keyword(s):  
Cell Lines ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cell

Hair follicle dermal papilla cell lines from p53-knockout mice

1997 ◽  
Vol 15 (1) ◽  
pp. 59-63 ◽  
Author(s):  
Chika Hanzawa ◽  
Koji Kobayashi ◽  
Yoko Hirabayashi ◽  
Tohru Inoue ◽  
Shin-ichi Aizawa ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Cell Lines ◽  
Knockout Mice ◽  
Dermal Papilla ◽  
Dermal Papilla Cell

Establishment and characterization of five immortalized human scalp dermal papilla cell lines

2018 ◽  
Vol 496 (2) ◽  
pp. 346-351 ◽  
Author(s):  
Mi Hee Kwack ◽  
Jung Min Yang ◽  
Gong Hee Won ◽  
Moon Kyu Kim ◽  
Jung Chul Kim ◽  
...  
Keyword(s):  
Cell Lines ◽  
Dermal Papilla ◽  
Dermal Papilla Cell

scholarly journals Dermal Papilla Cell-Derived Extracellular Vesicles Increase Hair Inductive Gene Expression in Adipose Stem Cells via β-Catenin Activation

Cells ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 202
Author(s):  
Taheruzzaman Kazi ◽  
Abir Nagata ◽  
Takatoshi Nakagawa ◽  
Takashi Matsuzaki ◽  
Shigeki Inui
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cell Differentiation ◽  
Extracellular Vesicles ◽  
Smooth Muscle Actin ◽  
Expression Patterns ◽  
Dermal Papilla ◽  
Pcr Analysis ◽  
Alpha Smooth Muscle Actin ◽  
Dermal Papilla Cell

Recently, extracellular vesicle (EV)-mediated cell differentiation has gained attention in developmental biology due to genetic exchange between donor cells and recipient cells via transfer of mRNA and miRNA. EVs, also known as exosomes, play a role in maintaining paracrine cell communication and can induce cell proliferation and differentiation. However, it remains unclear whether adipose-derived stem cells (ASCs) can adopt dermal papilla (DP)-like properties with dermal papilla cell-derived extracellular vesicles (DPC-EVs). To understand the effect of DPC-EVs on cell differentiation, DPC-EVs were characterized and incubated with ASCs, of monolayer and spheroid cell cultures, in combination with the CAO1/2FP medium specialized for dermal papilla cells (DPCs). DPC-like properties in ASCs were initially evaluated by comparing several genes and proteins with those of DPCs via real-time PCR analysis and immunostaining, respectively. We also evaluated the presence of hair growth-related microRNAs (miRNAs), specifically mir-214-5P, mir-218-5p, and mir-195-5P. Here, we found that miRNA expression patterns varied in DPC-EVs from passage 4 (P4) or P5. In addition, DPC-EVs in combination with CAP1/2FP accelerated ASC proliferation at low concentrations and propagated hair inductive gene expression for versican (vcan), alpha-smooth muscle actin (α-sma), osteopontin (opn), and N-Cam (ncam). Comparison between the expression of hair inductive genes (vcan, α-sma, ctnb, and others), the protein VCAN, α-SMA and β-Catenin (CTNB), and hair inductive miRNAs (mir-214-5P, mir-218-5p, and mir-195-5p) of DPC-EVs revealed similarities between P4 DPC-EVs-treated ASCs and DPCs. We concluded that early passage DPC-EVs, in combination with CAP1/2FP, enabled ASCs to transdifferentiate into DPC-like cells.

scholarly journals Canine Adipose-Derived Stem Cell Aggregates as a Viable Substitute to Actual Canine Dermal Papillae

2015 ◽  
Vol 38 (2) ◽  
pp. 167-173 ◽  
Author(s):  
Sohee Bae ◽  
Jina Kim ◽  
Li Li ◽  
Aeri Lee ◽  
Hyunjoo Lim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hair Follicle ◽  
Dermal Papilla ◽  
Human Medicine ◽  
Pcr Analysis ◽  
Molecular Characteristics ◽  
Dermal Papilla Cells ◽  
Dermatological Disease ◽  
Adipose Derived Stem Cell

Abstract Hair loss is a major dermatological disease in veterinary and human medicine. Active studies on hair regeneration with mesenchymal stem cells have been performed in an effort to solve the limitations of conservative treatments in human medicine. Our understanding of the canine hair follicle (HF), considering a useful model for the study of the human alopecia, is limited. This study was designed to broaden our understanding of canine dermal papilla (DP), and to reconstruct dermal papilla-like tissue (DPLT) using canine adipose-derived mesenchymal stem cells (AD-MSCs), as an alternative to actual DP. We cultured canine DPs, observed their culture patterns and compared their expression level of DP-related genes and proteins with those of DPLTs by performing RT-PCR analysis and Western blotting. Canine dermal papilla cells (DPCs) showed multilayer culture patterns with pseudo-papillae. Reconstruction of DPLTs was performed successfully. Not only were they morphologically similar to actual DPs, but we also observed similarities between DPCs and DPLTs in molecular characteristics. These findings suggested that DPLT was a viable substitute for DP. This study will not only be helpful for understanding the morphological and molecular characteristics of canine DPCs, but may also serve as a basis for understanding human hair follicle biology and potential therapeutic strategies for alopecia.

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