THE MICROBIOLOGICAL DETERMINATION OF THE ESSENTIAL AMINO ACIDS IN FISH PROTEIN

1956 ◽  
Vol 21 (1) ◽  
pp. 87-90 ◽  
Author(s):  
N. L. LAHIRY ◽  
B. E. PROCTOR
2007 ◽  
Vol 2007 ◽  
pp. 165-165
Author(s):  
M. Hassan Fathi Nasri ◽  
Mohsen Danesh Mesgaran ◽  
Reza Valizadeh ◽  
Ali Nikkhah

Whole soybean (SB) is used as a high energy-protein supplement for dairy cows, but the protein is highly degradable by rumen microbes. Various chemical and physical processing has been suggested to decrease ruminal protein degradability, that heat processing is the most commonly used physical method. Modern systems for protein evaluations in ruminants are moving in the direction of predicting absorption of amino acids from the small intestine, so the determination of intestinal digestibility of amino acids is of special importance particularly in heat-treated feedstuffs. The objective of this research was to elucidate the effects of roasting and steep-roasting on ruminal and post-ruminal disappearance of essential and non-essential amino acids (EAA and NEAA) of Iranian SB.


1986 ◽  
Vol 43 (11) ◽  
pp. 2320-2327 ◽  
Author(s):  
J. D. Castell ◽  
J. C. Kean

Many of the hypotheses and proposals presented here on the role of nutrition in lobster recruitment have been derived from research on other aquatic animals. Little research has focused on the determination of nutritional requirements of lobsters and even that has been primarily with small juveniles (36–1000 mg). Despite the tacit acknowledgement that maternal fecundity and egg viability are a function of quantity and quality of available food, little is known about the nutrition of reproductively mature lobsters. The importance of broodstock nutrition and possible methods for evaluating nutritional status are discussed. Information on protein, essential amino acids, vitamins, minerals, carbohydrates, and lipids is summarized. Additionally, some proposals for field application of nutritional response criteria used in the laboratory are discussed. Though research on larvae adult broodstock nutrition, effects of environmental factors (e.g. temperature, salinity, photoperiod, etc.), as well as many other aspects of nutrition is yet to be done, there is "good opportunity" for nutrition to be a significant part of the current lobster recruitment research program.


2004 ◽  
Vol 16 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Renato Rodrigues Ferreira ◽  
Ariane Vendemiatti ◽  
Lyndel Wayne Meinhardt ◽  
Peter John Lea ◽  
Ricardo Antunes Azevedo

Cereal seeds are poor in essential amino acids, particularly lysine, tryptophan and threonine. The amino acids lysine and threonine are synthesized in the aspartate pathway. Although most of the enzymes of the aspartate pathway have been isolated and characterized in higher plant species, the metabolism of lysine and threonine is totally unknown in sorghum. We have isolated two enzymes, aspartate kinase (AK) and homoserine dehydrogenase (HSDH) from sorghum. Optimum assay conditions were established for the determination of AK and HSDH activities. The highest level of activity was observed in immature seeds. AK was shown to be inhibited by threonine and lysine indicating the existence of at least two isoenzymes, one sensitive to threonine inhibition and the other sensitive to lysine inhibition with the latter being predominant in sorghum seeds. HSDH was shown to be inhibited by threonine indicating the existence of a threonine-sensitive HSDH, however, most of the activity was not inhibited by threonine, suggesting the existence of a second predominant isoenzyme of HSDH resistant to threonine inhibition.


2001 ◽  
Vol 75 (11) ◽  
pp. 5425-5428 ◽  
Author(s):  
Julie Dumonceaux ◽  
Caroline Goujon ◽  
Veronique Joliot ◽  
Pascale Briand ◽  
Uriel Hazan

ABSTRACT Seven mutations in the C2, V3, and C3 regions of gp120 are implicated in the tropism of the first CD4-independent human immunodeficiency virus type 1 isolate, m7NDK. Site-directed mutagenesis revealed that three amino acids are essential to maintain this tropism, one in the C2 region and two in the V3 loop. Two mutations implied N glycosylation modifications.


1975 ◽  
Vol 32 (3) ◽  
pp. 422-426 ◽  
Author(s):  
G. L. Rumsey ◽  
H. G. Ketola

Fry of Atlantic salmon (Salmo salar) fed a diet containing isolated fish protein grew significantly faster than those fed a diet containing casein without supplemental amino acids, and had improved feed conversion and lower mortality. Supplementing the casein diet with essential amino acids to the levels in the isolated fish protein diet significantly increased growth rate, reduced mortality, and improved feed conversion.When fingerling rainbow trout (S. gairdneri) were fed diets containing soybean meal as the sole source of protein, additions of amino acids to simulate those levels of essential amino acids in trout eggs and isolated fish protein significantly improved growth. Additions of methionine, lysine, histidine, and leucine individually and in several combinations had no effect.


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