scholarly journals Characterization of Thiobacillus thioparus isolated from an activated sludge bioreactor used for hydrogen sulfide treatment

2006 ◽  
Vol 101 (6) ◽  
pp. 1269-1281 ◽  
Author(s):  
V.L. Barbosa ◽  
S.D. Atkins ◽  
V.P. Barbosa ◽  
J.E. Burgess ◽  
R.M. Stuetz
Keyword(s):  
Hydrogen Sulfide ◽  
Activated Sludge ◽  
Thiobacillus Thioparus

scholarly journals Genetic and Immunochemical Characterization of Thiocyanate-Degrading Bacteria in Lake Water

2002 ◽  
Vol 68 (2) ◽  
pp. 942-946 ◽  
Author(s):  
Manabu Yamasaki ◽  
Yasuhiko Matsushita ◽  
Motonobu Namura ◽  
Hiroshi Nyunoya ◽  
Yoko Katayama
Keyword(s):  
Activated Sludge ◽  
Lake Water ◽  
Enrichment Culture ◽  
Soil Samples ◽  
Gene Encoding ◽  
Thiobacillus Thioparus ◽  
Immunochemical Characterization ◽  
Degrading Bacteria ◽  
Dominant Bacteria

ABSTRACT Natural aquatic and soil samples were screened for the presence of thiocyanate-degrading bacteria. Using thiocyanate supplementation, we established an enrichment culture containing such bacteria from lake water. The dominant bacteria had the scnC-LS5 gene encoding thiocyanate hydrolase, which was closely related to the enzyme found previously in Thiobacillus thioparus THI115 isolated from activated sludge.

Enrichment and characterization of an anaerobic methyl ethyl ketoxime degrading culture from an anoxic/anaerobic/aerobic activated sludge sequencing batch reactor

1998 ◽  
Vol 37 (4-5) ◽  
pp. 95-98 ◽  
Author(s):  
Nancy G. Love ◽  
Mary E. Rust ◽  
Kathy C. Terlesky
Keyword(s):  
Energy Source ◽  
Activated Sludge ◽  
Electron Acceptor ◽  
Sequencing Batch Reactor ◽  
Enrichment Culture ◽  
Batch Reactor ◽  
Nitrification Inhibitor ◽  
Time Frame ◽  
Methyl Ethyl

An anaerobic enrichment culture was developed from an anoxic/anaerobic/aerobic activated sludge sequencing batch reactor using methyl ethyl ketoxime (MEKO), a potent nitrification inhibitor, as the sole carbon and energy source in the absence of molecular oxygen and nitrate. The enrichment culture was gradually fed decreasing amounts of biogenic organic compounds and increasing concentrations of MEKO over 23 days until the cultures metabolized the oxime as the sole carbon source; the cultures were maintained for an additional 41 days on MEKO alone. Turbidity stabilized at approximately 100 mg/l total suspended solids. Growth on selective media plates confirmed that the microorganisms were utilizing the MEKO as the sole carbon and energy source. The time frame required for growth indicated that the kinetics for MEKO degradation are slow. A batch test indicated that dissolved organic carbon decreased at a rate comparable to MEKO consumption, while sulfate was not consumed. The nature of the electron acceptor in anaerobic MEKO metabolism is unclear, but it is hypothesized that the MEKO is hydrolyzed intracellularly to form methyl ethyl ketone and hydroxylamine which serve as electron donor and electron acceptor, respectively.

Characterization of the fate of lipids in activated sludge

2008 ◽  
Vol 20 (5) ◽  
pp. 536-542 ◽  
Author(s):  
Kangala B. Chipasa ◽  
Krystyna Mdrzycka
Keyword(s):  
Activated Sludge

scholarly journals Characterization of Norovirus-Binding Proteins (NoVBPs) Recovered from Activated Sludge Microorganisms

2007 ◽  
Vol 30 (12) ◽  
pp. 731-736
Author(s):  
Keishi WADA ◽  
Daisuke SANO ◽  
Takahiro IMAI ◽  
Tatsuo OMURA
Keyword(s):  
Activated Sludge ◽  
Binding Proteins

Estimation of Kinetic Parameters of Heterotrophic Biomass under Aerobic Conditions and Characterization of Wastewater for Activated Sludge Modelling

1992 ◽  
Vol 25 (6) ◽  
pp. 125-139 ◽  
Author(s):  
J. Kappeler ◽  
W. Gujer
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Kinetic Parameters ◽  
Specific Growth ◽  
Wastewater Treatment Plants ◽  
Simple Method ◽  
Batch Tests ◽  
Different Types ◽  
Heterotrophic Biomass

To predict the behaviour of biological wastewater treatment plants, the Activated Sludge Model No. 1 is often used. For the application of this model kinetic parameters and wastewater composition must be known. A simple method to estimate kinetic parameters of heterotrophic biomass and COD wastewater fractions is presented. With three different types of batch-tests these parameters and fractions can be determined by measuring oxygen respiration. Our measurements showed that the maximum specific growth rate µmax of heterotrophic biomass depends on temperature, reactor configuration and SRT. In typical wastewater treatment plants of Switzerland the amount of readily biodegradable substrate was generally small (about 9 % of the COD in primary effluent). The same method can also be used to determine kinetic parameters of nitrifying biomass.

Characterization of wastewater and activated sludge from European municipal wastewater treatment plants using the NUR test

1998 ◽  
Vol 38 (1) ◽  
pp. 303-310 ◽  
Author(s):  
V. Naidoo ◽  
V. Urbain ◽  
C. A. Buckley
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Municipal Wastewater Treatment ◽  
Batch Tests ◽  
Denitrification Kinetics ◽  
Municipal Wastewater Treatment Plants

Denitrification kinetics and wastewater characterization of eight different plants in Europe are discussed. Denitrification batch tests revealed three distinct rates except in the cases of Plaisir, Rostock and Orense where 4 rates were observed. The latter three plants revealed atypical rapid initial rates which were between 7 and 21 mgN/gVSS.h. All denitrification kinetics under non-limiting carbon conditions revealed fast first rates which ranged between 3.0 and 7.3 mgN/gVSS.h. Acetate was used to simulate denitrification kinetics with readily biodegradable COD present. Two subsequent rates were observed. Rates 2 and 3 ranged between 2 and 3 mgN/gVSS.h, and 1 and 2 mgN/gVSS.h, respectively. The RBCOD fraction varied between 10 and 19%, except for one of the plants where the value determined was 7%.

Physicochemical and Biological Treatability Studies on Surfactant Wastewaters

1992 ◽  
Vol 26 (1-2) ◽  
pp. 377-386 ◽  
Author(s):  
F. Çeçen
Keyword(s):  
Activated Sludge ◽  
Suspended Solids ◽  
Heavy Duty ◽  
Surface Active Agents ◽  
Surface Active ◽  
Liquid Type ◽  
Experimental Findings ◽  
Biological Treatability ◽  
Anionic Surface

Laboratory-scale physicochemical and biological treatability studies were performed on wastewaters discharged from an industry producing household and heavy-duty detergents of powder and liquid type. The characterization of effluents led to the conclusion that the industry was highly pollutant in terms of BOD5, COD, surfactant, phosphorus, oil-grease, suspended solids and pH. By the use of lime in the precipitation stage about 80 % of COD and more than 90 % of phosphorus and anionic surface active agents could be removed. Following the treatment with lime the biodegradation of these wastewaters was investigated in continuously fed activated sludge units. The biokinetic constants of the reaction were determined as k = 0.76 d−1, Ks = 972 mg/l COD, a = 0.58 mg O2/mg COD, b = 0.044 mg O2/mg MLVSS.d. The experimental findings were used in the design of a full-scale treatment system.

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