High Molecular Weight Aggregate Content of Heated and Unheated Factor VIII Products Determined by Fast-Protein Liquid Chromatography

Vox Sanguinis ◽  
1990 ◽  
Vol 58 (1) ◽  
pp. 30-34 ◽  
Author(s):  
J. Dawes ◽  
L. Freeman ◽  
N. J. Dawson ◽  
D. S. Pepper ◽  
T. W. Barrowcliffe
Keyword(s):  
Molecular Weight ◽  
Liquid Chromatography ◽  
Factor Viii ◽  
High Molecular Weight ◽  
Fast Protein Liquid Chromatography ◽  
Aggregate Content ◽  
High Molecular Weight Aggregate

scholarly journals High Molecular Weight Factor VIII Coagulant Activity in Cryoprecipitate and Polyethylene Glycol Precipitates

1977 ◽  
Author(s):  
K. A. Rickard ◽  
T. Exner ◽  
H. Kronenberg
Keyword(s):  
Molecular Weight ◽  
Polyethylene Glycol ◽  
Factor Viii ◽  
High Molecular Weight ◽  
Gel Filtration ◽  
Lower Molecular Weight ◽  
Human Antibody ◽  
High Molecular Weight Material ◽  
Coagulant Activity ◽  
Molecular Weight Form

Gel filtration of human plasma cryoprecipitate on Sepharose 2B indicated the molecular weight of factor VIII coagulant activity (VIIIc) to be significantly greater than that found in antihaemophilic concentrate. Polyethylene glycol at 3% concentration precipitated approximately half of the VIIIc from cryoprecipitate. This activity eluted as high molecular weight material on gel filtration. The addition of more polyethylene glycol to a concentration of 8% precipitated most of the remaining VIIIc from cryoprecipitate. This activity appeared to be of significantly lower molecular weight, approximately corresponding in elution volume to that observed for antihaemophilic concentrate. The possibility that an antibody to VIIIc generated in a patient treated with cryoprecipitate might be directed against the higher molecular weight form of factor VIII was investigated. However, no significant differences between the higher and lower molecular weight forms of factor VIII either in stability or in reactivity with human antibody to factor VIII were found.

Sign in / Sign up

Export Citation Format

Share Document