Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools

Vox Sanguinis ◽  
2008 ◽  
Vol 95 (4) ◽  
pp. 288-297 ◽  
Author(s):  
O. Rubin ◽  
D. Crettaz ◽  
G. Canellini ◽  
J.-D. Tissot ◽  
N. Lion
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells

Improved quantitative detection of biotin‐labeled red blood cells by flow cytometry

Transfusion ◽  
2019 ◽  
Vol 59 (8) ◽  
pp. 2691-2698 ◽  
Author(s):  
Albert D. Donnenberg ◽  
Tamir Kanias ◽  
Darrell J. Triulzi ◽  
Catherine J. Dennis ◽  
E. Michael Meyer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Quantitative Detection

Flow cytometry evaluation of red blood cells mimicking naturally occurring ABO subgroups after modification with variable amounts of function-spacer-lipid A and B constructs

Transfusion ◽  
2011 ◽  
Vol 52 (2) ◽  
pp. 247-251 ◽  
Author(s):  
Annika K. Hult ◽  
Tom Frame ◽  
Scott Chesla ◽  
Stephen Henry ◽  
Martin L. Olsson
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Lipid A ◽  
Naturally Occurring

scholarly journals Assessment of deformation of human red blood cells in flow cytometry: measurement and simulation of bimodal forward scatter distributions

2019 ◽  
Vol 10 (9) ◽  
pp. 4531
Author(s):  
Jonas Gienger ◽  
Hermann Gross ◽  
Volker Ost ◽  
Markus Bär ◽  
Jörg Neukammer
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Forward Scatter ◽  
Human Red Blood Cells

Trauma and Pregnancy: Is Flow Cytometry Detection and Quantification of Fetal Red Blood Cells Useful? A Retrospective Cohort Study.

2021 ◽  
Author(s):  
Misgav Rottenstreich ◽  
Reut Meir ◽  
Itamar Glick ◽  
Heli Alexandrony ◽  
Alon D Schwarz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cohort Study ◽  
Red Blood Cells ◽  
Pregnant Women ◽  
Retrospective Cohort Study ◽  
Blood Cells ◽  
Retrospective Cohort ◽  
Neonatal Outcome ◽  
Adverse Outcomes ◽  
Minor Trauma

Abstract Purpose: To assess whether positive flow cytometry quantification of fetal red blood cells is associated with adverse outcomes in cases of trauma during pregnancy.Methods: A retrospective cohort study, at a single tertiary center between 2013 and 2019. All pregnant women with viable gestation involved in trauma who underwent flow cytometry were included. Flow cytometry was considered positive (≥0.03/≥30 ml). Composite adverse maternal and neonatal outcomes were compared between cases with positive and negative flow cytometry test. Univariate and multivariate logistic regression analysis were performed to assess the role of flow cytometry in predicting adverse outcomes. Adjusted odds ratios (aORs) and 95% confidence intervals (CIs) were calculated.Results: During the study 1023 women met inclusion and exclusion criteria. The mechanisms of injury were motor vehicle accident in 387 women (38%), falls in 367 (36%), direct abdominal trauma in 353 (35%) and in 14 women (1%) other mechanism of injury. Among the cohort, 119 women (11.6%) had positive flow cytometry (≥0.03/≥30 ml) with median result of 0.03 [0.03-0.04], while 904 women (88.4%) had negative flow cytometry test result (≤0.03/≤30 ml) with median result of 0.01 [0.01-0.02]. Composite adverse outcome occurred in 8% of the women, with no difference in the groups with vs. without positive flow cytometry (4.2% vs. 8.5%; p=0.1). Positive flow cytometry was not associated with any adverse maternal or neonatal outcome. This was confirmed on a multivariate analysis. Conclusions: Flow cytometry result is not related to adverse maternal and fetal/neonatal outcome of women involved in minor trauma during pregnancy. We suggest that flow cytometry should not be routinely assessed in pregnant women involved in minor trauma.

The Role of Lactadherin in the Phagocytosis of Phosphatidylserine-Expressing Sickle Red Blood Cell by Macrophages.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3773-3773
Author(s):  
Swapan K. Dasgupta ◽  
Perumal Thiagarajan
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Blood Cells ◽  
Milk Fat ◽  
Integrin Αvβ3 ◽  
Fold Increase ◽  
Anionic Phospholipid ◽  
Sickle Red Blood Cells

Abstract Sickle cell anemia, the most common serious hemoglobinopathy, is associated with a markedly reduced life span of red blood cells due to their preferential clearance by macrophages. During polymerization of sickle hemoglobin, phosphatidylserine, an anionic phospholipid normally present exclusively on the inner leaflet of the membrane bilayer is exteriorized to outer leaflet. This exposure of phosphatidylserine is thought to be a tag for macrophage recognition. Lactadherin, also known as milk fat globule-EGF factor 8, is a phosphatidylserine-binding glycoprotein secreted by macrophages that promotes the engulfment of apoptotic cells. Here, we investigated the role of lactadherin in the phagocytosis of sickle red blood cells. The binding of fluorescein-lactadherin to normal and sickle red blood cells was studied by flow cytometry. We quantified the effect of lactadherin on phagocytosis of red blood cells by monocyte-derived macrophage. In normal individuals, less than 0.5% of red blood cells showed any binding to lactadherin when analyzed by flow cytometry. However, in sickle cell patients, circulating red blood cells showed 2 to 10- fold increase in lactadherin binding (P<0.0002). Lactadherin stimulated the phagocytosis of resting sickle red blood cells by macrophages but had no significant effect on the phagocytosis of normal red blood cells. Deoxygenation of sickle red blood cells further increased the lactadherin binding and phagocytosis. Antibodies to integrin αVβ3 also inhibited macrophage binding and phagocytosis. These results show lactadherin may play a major role in sickle red cell clearance by anchoring the phosphatidylserine-expressing sickle red blood cells to integrins on tissue macrophages.

Flow Cytometry in Detection of Fetal Red Blood Cells and Maternal F Cells to Identify Fetomaternal Hemorrhage

2016 ◽  
Vol 35 (6) ◽  
pp. 385-391 ◽  
Author(s):  
Mariela Granero Farias ◽  
Suzane Dal Bó ◽  
Simone Martins de Castro ◽  
Aline Reis da Silva ◽  
Joyce Bonazzoni ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Fetomaternal Hemorrhage ◽  
F Cells

scholarly journals Thalassemic DNA-Containing Red Blood Cells Are under Oxidative Stress

Anemia ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Mutaz Dana ◽  
Eugenia Prus ◽  
Eitan Fibach
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Reactive Oxygen ◽  
Oxidative Status ◽  
Oxygen Species ◽  
Dna Breakage ◽  
Erythroid Precursors

We studied the nature of enucleated RBCs containing DNA remnants, Howell-Jolly (HJ) RBCs and reticulocytes (retics), that are characteristically present in the circulation of thalassemic patients, especially after splenectomy. Using flow cytometry methodology, we measured oxidative status parameters of these cells in patients withβ-thalassemia. In each patient studied, these cells had higher content of reactive oxygen species and exposed phosphatidylserine compared with their DNA-free counterparts. These results suggest that oxidative stress in thalassemic developing erythroid precursors might, through DNA-breakage, generate HJ-retics and HJ-RBCs and that oxidative stress-induced externalization of phosphatidylserine is involved in the removal of these cells from the circulation by the spleen, a mechanism similar to that of the removal of senescent RBCs.

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