Sperm Motile Efficiency (SME) of Fresh Spermatozoa in a Hypotonic Medium Correlates with their Cryosurvival in Fertile Men and Infertility Patients (Predicting Test)/Die Spermien-Motilitätseffizienz (SME) von Spermien in einem hypotonen Medium korreliert

Andrologia ◽  
2009 ◽  
Vol 21 (4) ◽  
pp. 363-369 ◽  
Author(s):  
H.-J. Glander ◽  
H. Bock ◽  
P. Winiecki
Keyword(s):  
Hypotonic Medium ◽  
Infertility Patients

scholarly journals Effects of controlled ovarian stimulation on vascular barrier and endothelial glycocalyx: a pilot study

2021 ◽  
Author(s):  
Nikolai Hulde ◽  
N. Rogenhofer ◽  
F. Brettner ◽  
N. C. Eckert ◽  
I. Fetz ◽  
...  
Keyword(s):  
Ovarian Stimulation ◽  
Capillary Permeability ◽  
Serum Levels ◽  
Controlled Ovarian Stimulation ◽  
Endothelial Glycocalyx ◽  
Control Group ◽  
Vascular Integrity ◽  
Fluid Extravasation ◽  
Endothelial Surface ◽  
Infertility Patients

Abstract Purpose Controlled ovarian stimulation significantly amplifies the number of maturing and ovulated follicles as well as ovarian steroid production. The ovarian hyperstimulation syndrome (OHSS) increases capillary permeability and fluid extravasation. Vascular integrity intensely is regulated by an endothelial glycocalyx (EGX) and we have shown that ovulatory cycles are associated with shedding of EGX components. This study investigates if controlled ovarian stimulation impacts on the integrity of the endothelial glycocalyx as this might explain key pathomechanisms of the OHSS. Methods Serum levels of endothelial glycocalyx components of infertility patients (n=18) undergoing controlled ovarian stimulation were compared to a control group of healthy women with regular ovulatory cycles (n=17). Results Patients during luteal phases of controlled ovarian stimulation cycles as compared to normal ovulatory cycles showed significantly increased Syndecan-1 serum concentrations (12.6 ng/ml 6.1125th–19.1375th to 13.9 ng/ml 9.625th–28.975th; p=0.026), indicating shedding and degradation of the EGX. Conclusion A shedding of EGX components during ovarian stimulation has not yet been described. Our study suggests that ovarian stimulation may affect the integrity of the endothelial surface layer and increasing vascular permeability. This could explain key features of the OHSS and provide new ways of prevention of this serious condition of assisted reproduction.

Peritoneal fluid and serum steroids in infertility patients

1984 ◽  
Vol 42 (2) ◽  
pp. 237-242 ◽  
Author(s):  
Philip R. Lesorgen ◽  
Chung H. Wu ◽  
Paul J. Green ◽  
Benjamin Gocial ◽  
Leonard J. Lerner
Keyword(s):  
Peritoneal Fluid ◽  
Infertility Patients

Evaluating γH2AX in spermatozoa from male infertility patients

2015 ◽  
Vol 104 (3) ◽  
pp. 574-581 ◽  
Author(s):  
Hui-zhi Zhong ◽  
Fu-tong Lv ◽  
Xue-lian Deng ◽  
Ying Hu ◽  
Dan-ni Xie ◽  
...  
Keyword(s):  
Male Infertility ◽  
Infertility Patients

Laparoscopic proximal tubal division can preserve ovarian reserve for infertility patients with hydrosalpinges

2008 ◽  
pp. ???-??? ◽  
Author(s):  
Koji Nakagawa ◽  
Shirei Ohgi ◽  
Akira Nakashima ◽  
Takashi Horikawa ◽  
Minoru Irahara ◽  
...  
Keyword(s):  
Ovarian Reserve ◽  
Infertility Patients

scholarly journals Heterogeneity among microtubules of the cytoplasmic microtubule complex detected by a monoclonal antibody to alpha tubulin.

1984 ◽  
Vol 98 (3) ◽  
pp. 1017-1025 ◽  
Author(s):  
W C Thompson ◽  
D J Asai ◽  
D H Carney
Keyword(s):  
Differential Staining ◽  
Hypotonic Medium ◽  
Cytoplasmic Microtubule ◽  
Alpha Tubulin ◽  
Apparent Correlation ◽  
Double Label ◽  
Cytoplasmic Microtubules ◽  
Fibroblastic Cells ◽  
In Cells

Three monoclonal antibodies specific for tubulin were tested by indirect immunofluorescence for their ability to stain cytoplasmic microtubules of mouse and human fibroblastic cells. We used double label immunofluorescence to compare the staining patterns of these antibodies with the total microtubule complex in the same cells that were stained with a polyclonal rabbit antitubulin reagent. Two of the monoclonal antitubulin antibodies bound to all of the cytoplasmic microtubules but Ab 1-6. 1 bound only a subset of cytoplasmic microtubules within individual fixed cells. Differential staining patterns were observed under various fixation conditions and staining protocols, in detergent-extracted cytoskeletons as well as in whole fixed cells. At least one physiologically defined subset of cytoplasmic microtubules, those remaining in cells pretreated for 1 h with 5 microM colcemid, appeared to consist entirely of Ab 1-6. 1 positive microtubules. The same was not true of the microtubules that remained in either cold-treated cells or in cells that had been exposed to hypotonic medium. The demonstration of antigenic differences among microtubules within single fixed cells and the apparent correlation of this antigenic difference with at least one "physiologically" defined subset suggests that mechanisms exist for the differential assembly or postassembly modification of individual microtubules in vivo, which may endow them with different physical or functional properties.

scholarly journals SPERM CASPASES BECOME MORE ACTIVATED IN INFERTILITY PATIENTS THAN IN HEALTHY DONORS DURING CRYOPRESERVATION

2005 ◽  
Vol 51 (6) ◽  
pp. 449-460 ◽  
Author(s):  
S. Grunewald ◽  
U. Paasch ◽  
K. Wuendrich ◽  
H.-J. Glander
Keyword(s):  
Healthy Donors ◽  
Infertility Patients

Spontaneous loss of a co-twin and the risk of birth defects after assisted conception

2016 ◽  
Vol 7 (6) ◽  
pp. 678-684 ◽  
Author(s):  
M. J. Davies ◽  
A. R. Rumbold ◽  
M. J. Whitrow ◽  
K. J. Willson ◽  
W. K. Scheil ◽  
...  
Keyword(s):  
Birth Defects ◽  
Pregnancy Loss ◽  
Multiple Pregnancy ◽  
Fetal Loss ◽  
South Australia ◽  
Increased Risk ◽  
Study Population ◽  
Infertility Patients ◽  
Twin Pregnancies ◽  
Singleton Pregnancies

The study of very early pregnancy loss is impractical in the general population, but possible amongst infertility patients receiving carefully monitored treatments. We examined the association between fetal loss and the risk of birth defects in the surviving co-twin in a retrospective cohort study of infertility patients within an infertility clinic in South Australia from January 1986 to December 2002, linked to population registries for births, terminations and birth defects. The study population consisted of a total of 5683 births. Births from singleton pregnancies without loss were compared with survivors from (1) pregnancies with an empty fetal sac at 6–8 weeks after embryo transfer, (2) fetal loss subsequent to 8-week ultrasound and (3) multiple pregnancy continuing to birth. Odds ratios (OR) for birth defects were calculated with adjustment for confounders. Amongst infertility patients, the prevalence of birth defects was 7.9% for all twin pregnancies without fetal loss compared with 14.6% in pregnancies in which there had been an empty sac at ultrasound, and 11.6% for pregnancies with fetal loss after 6–8 weeks. Compared with singleton pregnancies without loss, the presence of an empty sac was associated with an increased risk of any defect (OR=1.90, 95% confidence intervals (CI)=1.09–3.30) and with multiple defects (OR=2.87, 95% CI=1.31–6.28). Twin pregnancies continuing to birth without loss were not associated with an overall increased prevalence of defects. We conclude that the observed loss of a co-twin by 6–8 weeks of pregnancy is related to the risk of major birth defects in the survivor.

Clinical and psychological features of acne women with infertility

2017 ◽  
pp. 118-122
Author(s):  
O.I. Zadnipryanaya ◽  
Keyword(s):  
Quality Of Life ◽  
Emotional Disorders ◽  
Social Effect ◽  
Sexually Transmitted ◽  
History Of ◽  
Atrophic Scars ◽  
Infertility Patients ◽  
Psychological Features ◽  
Infertile Patients

The objective: the study of clinical and psychological features of acne and quality of life in women with infertility. Patients and methods. 151 patients were included in the study: 111 with acne and infertility, 40 fertile women with acne entered the comparison group. An anamnesis, a dermatological and gynecological status, a hormonal background, an assessment of the psycho-emotional state and quality of life of patients (a Dermatological Index of Quality of Life – DIQL, the Hospital Scale of Anxiety and Depression – HADS), and the psychological and social effect of acne (APSEA questionnaire) were evaluated. Results. Infertile women with acne reliably recorded open and closed comedones, more often papules, stagnant spots, hyperpigmentation, enlarged pores, as well as atrophic scars were more often detected. They are characterized by a later menarche, an early onset of sexual activity. In 56.8% of infertile patients in the history of the disease, sexually transmitted diseases, abortions (8.1%), miscarriages and stagnant pregnancies (12.6%), ectopic pregnancy (4.5%). It was found that infertility lasts on average 4.9±3.6 years, with primary infertility diagnosed in 69.4% of cases. The endocrine nature of infertility was confirmed in 48 (43.2%) patients, tubo-peritoneal – in 63 (56.8%) of the women. It was found that the disease has a very strong effect on the quality of their life (13.3±6.2 points), causes a strong psychological and social effect (87.8±28.1 points), clinically expressed anxiety (11.8±4.4 points) and depressiveness (11.6±3.8 points). Conclusion. Acne in infertile patients in addition to clinical features is accompanied by significant violations of the psycho-emotional sphere and a decrease in the quality of life. Key words: women with acne and infertility, quality of life, psycho-emotional disorders, psychological and social effect of acne.

Study of Influence on Infertility

1970 ◽  
Vol 1 (2) ◽  
Author(s):  
Shi Huihui
Keyword(s):  
Risk Factors ◽  
Reproductive Health ◽  
Incidence Rate ◽  
Influential Factors ◽  
Developmental Abnormality ◽  
Secondary Infertility ◽  
Child Bearing ◽  
Related Risk ◽  
Infertility Patients ◽  
The City

For objective, analyzing the pathogenesis of infertility patients in Zhengzhou and exploring the relevant influential factors. Method: selecting 264 infertility patients in our hospital and peripheral hospitals in the city from March of 2015 to October of 2016 to carry out relevant investigation & research so as to analyze the major pathogeny and related risk factors of 264 infertility patients. For result, for infertility patients, the occurrence of infertility is common in female whether it is primary or secondary. The primary infertility is usually caused by uterus factors, showing the congenital developmental abnormality of uterus. However, the primary cause of secondary infertility is tubal nowhere, commonly and mainly showing frequent abortion frequency.As to male infertility, the idiopathic infertility is mainly related to asthenospermia while the secondary infertility is mainly related to asthenospermia and oligospermia. Conclusion: clinically, there are more causes of infertility. Thus, the reproductive health education and direction need to be strengthened and completed on the male and female during the child-bearing period, and the timely and professional direction needs to be given to the patients with low incidence of infertility to diagnose and treat as early as possible so as to lower the incidence rate of infertility, worthy of attention.

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