The clonal structure of Bacillus thuringiensis isolates from north-east Poland does not correlate with their cry gene diversity

2005 ◽  
Vol 7 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Izabela Swiecicka ◽  
Jacques Mahillon
2007 ◽  
Vol 55 (3) ◽  
pp. 217-222 ◽  
Author(s):  
Edyta Konecka ◽  
Adam Kaznowski ◽  
Jadwiga Ziemnicka ◽  
Kazimierz Ziemnicki ◽  
Halina Paetz

North East India is extremely rich in natural resources and is a biodiversity hotspot. Many plant species, including Citrus are known to have originated from this region. Citus macroptera Mont. is a wild, endangered species which have been found to exist naturally in various parts of this region, including Meghalaya. To access the genetic variability among genotypes and their phylogeny, 30 genotypes of wild C. macroptera Mont. were collected from Garo Hills of Meghalaya. Single primer based DNA markers viz. RAPD, ISSR, DAMD were utilized to ascertain genetic diversity. The percentage polymorphic bands for RAPD, ISSR, DAMD were found to be 97.71%, 94.67% and 100% respectively. ISSR showed the highest values for both RP (7.67) and MI (5.03) highlighting its efficacy in determining genetic variations. A concatenated approach, Single Primer Amplification Reaction (SPAR) was also followed to assess their genetic diversity. Dendogram generated from SPAR data showed that the South-West Garo Hills population is the most recently evolved amongst all others while West Garo Hills collections retain an ancestral position in the evolutionary time-frame. Population genetics parametres such as Gene flow (Nm) and the diversity among populations (GST) were found to be 1.9894 and 0.2009 respectively. Gene flow estimates (Nm>1) suggests appreciable gene flow in the populations. AMOVA data further supported this with high percentage of variations (92%) within populations whereas variations among populations were about 8% only. Shannon’s information index (I) values and Nei’s gene diversity (h) varied between 0.303-0.423and 0.201-0.285 respectively. The use of SPAR method yields a clear and concise picture of the underlying genetic variabilities, and a detailed and comprehensive data analysis will help conceive efficient and sustainable conservation strategies for this important plant.


Toxins ◽  
2014 ◽  
Vol 6 (7) ◽  
pp. 2194-2209 ◽  
Author(s):  
Chao Deng ◽  
Qi Peng ◽  
Fuping Song ◽  
Didier Lereclus

1967 ◽  
Vol 13 (1) ◽  
pp. 81-91 ◽  
Author(s):  
J. M. Perron ◽  
W. A. Smirnoff ◽  
L. Huot

This study is concerned with the effects of aluminium, arsenic, boron, cobalt, copper, fluorine, iron, iodine, lithium, magnesium, molybdenum, and zinc on Bacillus thuringiensis var. thuringiensis Berliner. Addition of small quantities of the elements to the culture medium stimulated growth of the Bacillus. The effect was particularly marked during cell division, as revealed by an increase in area of the clones. The toxic effects of certain concentrations of the elements was also revealed during cell division, which, as indicated by the clonal area, was adversely affected by B, Cu, I, Mg, and Zn. F, Fe, Li, and Mo effected retardation of the cell division by as much as 12 to 48 hours. Al, As, Cu, and Mg both retarded and reduced cell division. By determining the relation between the effective limiting doses established, we were able to divide the elements into four groups. The toxicity of the elements was also revealed by its effects on the germination of the spores, clonal structure, form of the cells, time of sporulation, synthesis of the toxin-crystal, and cellular lysis.


2005 ◽  
Vol 51 (11) ◽  
pp. 988-995 ◽  
Author(s):  
Naoya Wasano ◽  
Hiroyuki Saitoh ◽  
Minoru Maeda ◽  
Akira Ohgushi ◽  
Eiichi Mizuki ◽  
...  

A novel δ-endotoxin gene from a lepidopteran-specific Bacillus thuringiensis serovar galleriae strain was cloned, and the full sequence of the cry gene was determined. The cloned 6.5-kb DNA fragment included the full sequence of the cry gene and three open reading frames located upstream of the cry gene. The gene, designated cry9Ec1, encodes a polypeptide of 1154 amino acid residues with a predicted molecular weight of 130 237. The deduced amino acid sequence of the Cry9Ec1 protein had the highest homology (77.7%) with the Cry9Ea1 protein when compared with existing Cry proteins. The expression, in an acrystalliferous B. thuringiensis strain, of the cry9Ec1 gene was high when controlled by the cyt1A2 promoter, leading to the formation of large spherical inclusions. The purified crystals from the recombinant strain were toxic when tested against two lepidopteran species, Bombyx mori and Plutella xylostella. However, the Cry9Ec1 protein gave no toxicity against Spodoptera litura, Spodoptera exigua, Plodia interpunctella, Helicoverpa zea, and Culex pipiens molestus.Key words: Bacillus thuringiensis serovar galleriae, Cry9Ec1 protein, lepidopteran-specific δ-endotoxin, Plutella xylostella.


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