Multilocus sequence typing for phylogenetic view and vip gene diversity of Bacillus thuringiensis strains of the Assam soil of North East India

Author(s):  
Mihir Rabha ◽  
Sumita Acharjee ◽  
Bidyut Kumar Sarmah

North East India is extremely rich in natural resources and is a biodiversity hotspot. Many plant species, including Citrus are known to have originated from this region. Citus macroptera Mont. is a wild, endangered species which have been found to exist naturally in various parts of this region, including Meghalaya. To access the genetic variability among genotypes and their phylogeny, 30 genotypes of wild C. macroptera Mont. were collected from Garo Hills of Meghalaya. Single primer based DNA markers viz. RAPD, ISSR, DAMD were utilized to ascertain genetic diversity. The percentage polymorphic bands for RAPD, ISSR, DAMD were found to be 97.71%, 94.67% and 100% respectively. ISSR showed the highest values for both RP (7.67) and MI (5.03) highlighting its efficacy in determining genetic variations. A concatenated approach, Single Primer Amplification Reaction (SPAR) was also followed to assess their genetic diversity. Dendogram generated from SPAR data showed that the South-West Garo Hills population is the most recently evolved amongst all others while West Garo Hills collections retain an ancestral position in the evolutionary time-frame. Population genetics parametres such as Gene flow (Nm) and the diversity among populations (GST) were found to be 1.9894 and 0.2009 respectively. Gene flow estimates (Nm>1) suggests appreciable gene flow in the populations. AMOVA data further supported this with high percentage of variations (92%) within populations whereas variations among populations were about 8% only. Shannon’s information index (I) values and Nei’s gene diversity (h) varied between 0.303-0.423and 0.201-0.285 respectively. The use of SPAR method yields a clear and concise picture of the underlying genetic variabilities, and a detailed and comprehensive data analysis will help conceive efficient and sustainable conservation strategies for this important plant.


2014 ◽  
Vol 12 (3) ◽  
pp. 264-272 ◽  
Author(s):  
Somnath Roy ◽  
A. Banerjee ◽  
A. Pattanayak ◽  
S. S. Roy ◽  
R. S. Rathi ◽  
...  

Rice landraces have been developed through artificial selection imposed by farmers during the long-term domestication process. Although the global rice diversity is well characterized, few studies have conducted an in-depth analysis of genetic diversity on a local scale. In India, there are many locally adapted non-Basmati aromatic rice landraces in which the pace of improvement is slow, despite their high economic values. The aromatic and quality rice landraces of Manipur, locally calledChakhao(delicious), are important, considering their high economic and cultural values. To conserve and encourage improvement of these landraces, we collectedChakhaoaccessions from eight districts of Manipur. The aim of the current study was to investigate the level of genetic diversity and structure of 37Chakhaolandraces based on genotyping with 47 microsatellite markers. The genetic diversity analysis revealed high gene diversity (0.673) within theChakhaopopulation, with values ranging from 0.303 (Poireiton) to 0.471 (mixedChakhao). TheChakhaorice accessions could be divided into six subgroups based on genetic structure analyses. The population structure derived from the STRUCTURE analysis largely correlated with the farmers' classification ofChakhaolandraces. The results of genetic diversity analyses and the indigenous knowledge of the names and use ofChakhaolandraces would facilitate the conservation and utilization of this unique genetic resource.


3 Biotech ◽  
2017 ◽  
Vol 7 (5) ◽  
Author(s):  
Mihir Rabha ◽  
Shaswati Sharma ◽  
Sumita Acharjee ◽  
Bidyut Kumar Sarmah

2020 ◽  
Vol 8 (2) ◽  
pp. 13
Author(s):  
Ankita Pandey

Guwahati derives its name from the Assamese word “Guwa” means areca nut and “Haat” means market. However, the modern Guwahati had been known as the ancient Pragjyotishpura and was the capital of Assam under the Kamrupa kingdom. A beautiful city Guwahati is situated on the south bank of the river Bramhaputra. Moreover, It is known as the largest city in the Indian state of Assam and also the largest metropolis in North East India. It has also its importance as the gateway to the North- East India. Assamese and English are the spoken languages in Guwahati.  In 1667, the Mogul forces were defeated in the battle by the Ahom forces commanded by Lachut Barphukan. Thus, in a sense Guwahati became the bone of contention among the Ahoms, Kochas and the Moguls during the medieval period.  Guwahati the administrative headquarters of Lower Assam with a viceroy or Barbhukan was made by the Ahom king.  Since 1972 it has been the capital of Assam. The present paper will discuss the changes happened in Guwahati over the period of late 1970s till the present time. It will focus on the behavior of people, transformed temples, Panbazar of the city, river bank of Bramhaputra, old Fancy Bazaar, chaotic ways, festivals and seasons including a fifth man made season etc. It will also deal how over the years a city endowed with nature’s gifts and scenic views, has been changing as “a dirty city”. Furthermore, it will also present the insurgencies that have barged into the city. The occurrence of changes will be discussed through the perspective and point of view of Srutimala Duara as presented in her book Mindprints of Guwahati.


2019 ◽  
Vol 5 (4) ◽  
pp. 14-20
Author(s):  
Ms. Cheryl Antonette Dumenil ◽  
Dr. Cheryl Davis

North- East India is an under veiled region with an awe-inspiring landscape, different groups of ethnic people, their culture and heritage. Contemporary writers from this region aspire towards a vision outside the tapered ethnic channel, and they represent a shared history. In their writings, the cultural memory is showcased, and the intensity of feeling overflows the labour of technique and craft. Mamang Dai presents a rare glimpse into the ecology, culture, life of the tribal people and history of the land of the dawn-lit mountains, Arunachal Pradesh, through her novel The Legends of Pensam. The word ‘Pensam’ in the title means ‘in-between’,  but it may also be interpreted as ‘the hidden spaces of the heart’. This is a small world where anything can happen. Being adherents of the animistic faith, the tribes here believe in co-existence with the natural world along with the presence of spirits in their forests and rivers. This paper attempts to draw an insight into the culture and gender of the Arunachalis with special reference to The Legends of Pensam by Mamang Dai.


GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 341-348
Author(s):  
Dr. Mini Jain ◽  
Dr. Mini Jain

In India, higher education is a need of hour. The excellence of Higher Edification decides the production of skilled manpower to the nation. Indian education system significantly teaching has not been tested too economical to form youths of our country employable in line with the requirement of job market. Despite the rise in range of establishments at primary, secondary and tertiary level our young educated folks don't seem to be capable of being used and recovering job opportunities. Reason being they need not non-heritable such skills essential for demand of the duty market. The present study is aimed at analyzing the status of higher education institutions in terms of Infrastructure, various courses of the institute, quality Initiatives and skill development program offered by the Institutes, in the North-East India region, so as to see whether the Higher Educational Institutes of this region are in the process of gradually developing the skills of the students in attaining excellence. The paper also laid emphasis on the measures adopted by these institutes for quality improvement, and to find out their role in combating the adversity acclaimed in the region, since this region’s development is impeded by certain inherent difficulties However, this paper focuses attention on high quality education with special emphasis on higher education for forward linkages through value addition.


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