Limb development in pouch young of the brushtail possum (Trichosurus vulpecula) and tammar wallaby (Macropus eugenii)

2006 ◽  
Vol 0 (0) ◽  
pp. 060606025751023-???
Author(s):  
R. G. Lentle ◽  
M. C. Kruger ◽  
D. J. Mellor ◽  
M. Birtles ◽  
P. J. Moughan
Keyword(s):  
Limb Development ◽  
Tammar Wallaby ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Macropus Eugenii

scholarly journals 62. Sperm proacrosin/acrosin system in two marsupial species, the brushtail possum (Trichosurus vulpecula) and the tammar wallaby (Macropus eugenii)

2003 ◽  
Vol 15 (9) ◽  
pp. 62
Author(s):  
K. S. Sidhu ◽  
K. E. Mate ◽  
F. C. Molinia ◽  
J. C. Rodger
Keyword(s):  
Tammar Wallaby ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Macropus Eugenii ◽  
Marsupial Species

scholarly journals Successful fertilization after superovulation and laparoscopic intrauterine insemination of the brushtail possum, Trichosurus vulpecula, and tammar wallaby, Macropus eugenii

Reproduction ◽  
1998 ◽  
Vol 113 (1) ◽  
pp. 9-17 ◽  
Author(s):  
F. C. Molinia ◽  
R. J. Gibson ◽  
A. M. Brown ◽  
A. M. Glazier ◽  
J. C. Rodger
Keyword(s):  
Intrauterine Insemination ◽  
Tammar Wallaby ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Macropus Eugenii

scholarly journals Acrosome formation during sperm transit through the epididymis in two marsupials, the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula)

1999 ◽  
Vol 194 (2) ◽  
pp. 223-232
Author(s):  
MINJIE LIN ◽  
JOHN C. RODGER
Keyword(s):  
Spatial Information ◽  
Dorsal Surface ◽  
Tammar Wallaby ◽  
Sperm Head ◽  
The Body ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Macropus Eugenii ◽  
Acrosomal Membrane ◽  
Acrosome Formation

In certain Australian marsupials including the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula), formation of the acrosome is not completed in the testis but during a complex differentiation process as spermatozoa pass through the epididymis. Using transmission and scanning electron microscopy this paper defined the process of acrosome formation in the epididymis, providing temporal and spatial information on the striking reorganisation of the acrosomal membranes and matrix and of the overlying sperm surface involved. On leaving the testis wallaby and possum spermatozoa had elongated ‘scoop’-shaped acrosomes projecting from the dorsal surface of the head. During passage down the epididymis, this structure condensed into the compact button-like organelle found on ejaculated spermatozoa. This condensation was achieved by a complex process of infolding and fusion of the lateral projections of the ‘scoop’. In the head of the epididymis the rims of the lateral scoop projections became shorter and thickened and folded inwards, to eventually meet midway along the longitudinal axis of the acrosome. As spermatozoa passed through the body of the epididymis the lateral projections fused together. Evidence of this fusion of the immature outer acrosomal membrane is the presence of vesicles within the acrosomal matrix which persist even in ejaculated spermatozoa. When spermatozoa have reached the tail of the epididymis the acrosome condenses into its mature form, as a small button-like structure contained within the depression on the anterior end of the nucleus. During the infolding process, the membranes associated with the immature acrosome are either engulfed into the acrosomal matrix (outer acrosomal membrane), or eliminated from the sperm head as tubular membrane elements (cytoplasmic membrane). Thus the surface and organelles of the testicular sperm head are transient structures in those marsupials with posttesticular acrosome formation and this must be taken into consideration in attempts to dissect the cell and molecular biology of fertilisation.

Changes in milk carbohydrates during lactation in the common brushtail possum, Trichosurus vulpecula (Marsupialia:Phalangeridae)

1989 ◽  
Vol 1 (4) ◽  
pp. 309 ◽  
Author(s):  
EA Crisp ◽  
PE Cowan ◽  
M Messer
Keyword(s):  
Tammar Wallaby ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Macropus Eugenii ◽  
Qualitative And Quantitative ◽  
Milk Samples ◽  
Nucleotide Pyrophosphatase ◽  
The Common ◽  
Quantitative Changes ◽  
Beta Galactosidase

Milk samples (186) were obtained at various stages of lactation from 27 common brushtail possums (Trichosurus vulpecula). Qualitative and quantitative changes in the milk carbohydrates during early and mid-lactation were similar to those previously seen in other marsupials; the principal carbohydrate was lactose early in lactation and higher oligosaccharides in mid-lactation, and the hexose concentration reached a peak during mid-lactation. However, the late-lactation milk was unusual in that the carbohydrate was mainly lactose and its concentration remained relatively high (3.5 to 5.5%). In contrast to earlier findings on the milk of the tammar wallaby (Macropus eugenii), little or no nucleotide pyrophosphatase, beta-galactosidase and alkaline phosphatase activities were detected late in lactation.

Pellet-freezing spermatozoa of two marsupials: the tammar wallaby, Macropus eugenii, and the brushtail possum, Trichosurus vulpecula

1996 ◽  
Vol 8 (4) ◽  
pp. 681 ◽  
Author(s):  
FC Molinia ◽  
JC Rodger
Keyword(s):  
Room Temperature ◽  
Tammar Wallaby ◽  
Brushtail Possum ◽  
Vitro Fertilization ◽  
Macropus Eugenii ◽  
Progressive Motility ◽  
Viable Population ◽  
Phosphate Buffered Saline ◽  
Breeding Techniques

A protocol was developed for pellet-freezing spermatozoa of the tammar wallaby and the brushtail possum. Seren was collected by electro-ejaculation and wallaby spermatozoa were washed by 'swim-up' into phosphate-buffered saline (PBS), whereas possum spermatozoa were not washed. Wallaby spermatozoa were screened for toxicity in diluents containing a range of cryoprotectants (0-10%): dimethyl sulfoxide (DMSO), ethylene glycol and propanediol. Possum spermatozoa were tolerant of diluents containing 17.5% glycerol. Wallaby and possum spermatozoa were diluted 1:1 with the most promising cryoprotective diluents (final concentrations in PBS: possum, 17.5% glycerol; wallaby, 7.5% glycerol + 10% DMSO) and, after 5 min equilibration at room temperature, were pellet-frozen. Pellets were thawed (35 degrees C) and wallaby spermatozoa were washed by centrifugation (200 g for 5 min) and resuspended in PBS to minimize cryoprotectant toxicity. A high proportion of possum spermatozoa was recovered after freezing (67.5%), having good progressive motility (3.6 on a 0-5 scale). The progressive motility of frozen-thawed wallaby spermatozoa was also high (3.0), but only 10% of motile spermatozoa were recovered. The pellet-freezing method in conjunction with the post-thaw washing procedure (wallaby) may produce a viable population of cryopreserved marsupial spermatozoa suitable for use in assisted-breeding techniques such as in vitro fertilization and artificial insemination.

scholarly journals Changes in Prolactin in Peripheral Plasma during Lactation in the Brushtail Possum Trichosurus vulpecula

1986 ◽  
Vol 39 (2) ◽  
pp. 171 ◽  
Author(s):  
LA Hinds ◽  
PA Janssens
Keyword(s):  
Dose Response ◽  
Brushtail Possum ◽  
Plasma Prolactin ◽  
Trichosurus Vulpecula ◽  
Response Curves ◽  
Thyrotrophin Releasing Hormone ◽  
Male And Female ◽  
Single Intravenous Injection ◽  
Macropus Eugenii ◽  
Peripheral Plasma

A heterologous double-antibody radioimmunoassay has been validated for prolactin in plasma and pituitary preparations of T. vulpecula. Serial dilutions of crude pituitary homogenates and plasmas from several marsupials and purified prolactin from the tammar, Macropus eugenii, showed parallel dose response curves. In both male and female possums plasma prolactin concentrations increased in response to a single intravenous injection of thyrotrophin releasing hormone.

Ontogeny and pathway of formation of 5α-androstane-3α,17β-diol in the testes of the immature brushtail possum Trichosurus vulpecula

2005 ◽  
Vol 17 (6) ◽  
pp. 603 ◽  
Author(s):  
Jean D. Wilson ◽  
Geoffrey Shaw ◽  
Marilyn B. Renfree ◽  
Richard J. Auchus ◽  
Michael W. Leihy ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Tammar Wallaby ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Mature Adults ◽  
Immature Male ◽  
Marsupial Species ◽  
Age Range

The testicular androgen 5α-androstane-3α,17β-diol (androstanediol) mediates virilisation in pouch young of a marsupial, the tammar wallaby, and is the principal androgen formed in immature rodent testes. To chart the pattern of androstanediol formation in another marsupial species, the testes or fragments of testes from brushtail possums (Trichosurus vulpecula) that spanned the age range from early pouch young to mature adults were incubated with 3H-progesterone and the products were identified by high-performance liquid chromatography. The only 19-carbon steroids identified in pouch young and adult testes were the Δ4-3-keto-steroids testosterone and androstenedione. However, androstanediol and another 5α-reduced androgen (androsterone) were synthesised by testes from Day 87–200 males and these appeared to be formed from the 5α-reduction and 3-keto reduction of testosterone and androstenedione. In the prostate and glans penis of the immature male, 3H-androstanediol was converted to dihydrotestosterone. We conclude that the timing of androstanediol formation in the possum testis resembles the process in rodents rather than in the tammar wallaby and that any androstanediol in the circulation probably acts in target tissues via conversion to dihydrotestosterone.

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