Examining the large-scale convergence of photosynthesis-weighted tree leaf temperatures through stable oxygen isotope analysis of multiple data sets

ABSTRACT How to achieve protein diversity by genome and transcriptome processing is essential for organismal complexity and adaptation. The present work identifies that the macronuclear genome of Halteria grandinella, a cosmopolitan unicellular eukaryote, is composed almost entirely of gene-sized nanochromosomes with extremely short nongenic regions. This challenges our usual understanding of chromosomal structure and suggests the possibility of novel mechvanisms in transcriptional regulation. Comprehensive analysis of multiple data sets reveals that Halteria transcription dynamics are influenced by: (i) nonuniform nanochromosome copy numbers correlated with gene-expression level; (ii) dynamic alterations at both the DNA and RNA levels, including alternative internal eliminated sequence (IES) deletions during macronucleus formation and large-scale alternative splicing in transcript maturation; and (iii) extremely short 5′ and 3′ untranslated regions (UTRs) and universal TATA box-like motifs in the compact 5′ subtelomeric regions of most chromosomes. This study broadens the view of ciliate biology and the evolution of unicellular eukaryotes, and identifies Halteria as one of the most compact known eukaryotic genomes, indicating that complex cell structure does not require complex gene architecture.

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Isotopes, Migration, and Sex

Bioarchaeology of Frontiers and Borderlands ◽

10.5744/florida/9781683400844.003.0005 ◽

2019 ◽

pp. 83-106

Author(s):

Amanda T. Groff ◽

Tosha L. Dupras

Keyword(s):

Oxygen Isotope ◽

Isotope Analysis ◽

Cultural Identities ◽

Nile Valley ◽

Stable Oxygen Isotope ◽

Textual Evidence ◽

Work Related ◽

Economic Practices ◽

Stable Oxygen ◽

Definition Of

The Egyptian oases were dynamic borderlands where culture, economic practices, and politics diverged from the Nile Valley. The cultural identities of the individuals inhabiting these frontiers during the Romano-Christian era (50–450CE) are predominantly lost to history, save for scant textual sources that describe socioeconomic activities. In this chapter, we explore these identities further by utilizing stable oxygen isotope analysis in conjunction with textual sources to discuss the mobility of adults from the Kellis 2 Cemetery, Dakhleh Oasis, Egypt. Results from this analysis indicate females came from isotopically similar environments and were stationary, while males were migrating more frequently for work-related activities. These data complement the limited textual evidence allowing for more detailed reconstruction of economics, kinship, and residence patterns during the Romano-Christian era, and lend to a definition of Egyptian frontier identity.

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Stable oxygen isotope analysis confirms natural recruitment of Lake Michigan-origin lake trout (Salvelinus namaycush) to the adult life stage

Fisheries Research ◽

10.1016/j.fishres.2017.01.013 ◽

2017 ◽

Vol 190 ◽

pp. 15-23 ◽

Cited By ~ 6

Author(s):

Sean Landsman ◽

Jeffrey A. Stein ◽

Gregory Whitledge ◽

Steven R. Robillard

Keyword(s):

Oxygen Isotope ◽

Lake Michigan ◽

Lake Trout ◽

Life Stage ◽

Isotope Analysis ◽

Adult Life ◽

Salvelinus Namaycush ◽

Stable Oxygen Isotope ◽

Stable Oxygen ◽

Natural Recruitment

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FragMAX: the fragment-screening platform at the MAX IV Laboratory

Acta Crystallographica Section D Structural Biology ◽

10.1107/s205979832000889x ◽

2020 ◽

Vol 76 (8) ◽

pp. 771-777 ◽

Cited By ~ 1

Author(s):

Gustavo M. A. Lima ◽

Vladimir O. Talibov ◽

Elmir Jagudin ◽

Céleste Sele ◽

Maria Nyblom ◽

...

Keyword(s):

Large Scale ◽

Structure Refinement ◽

Fourth Generation ◽

Data Sets ◽

Fragment Screening ◽

Efficient Management ◽

Multiple Data ◽

Crystal Fragment ◽

Multiple Data Sets ◽

Screening Platform

Advances in synchrotron storage rings and beamline automation have pushed data-collection rates to thousands of data sets per week. With this increase in throughput, massive projects such as in-crystal fragment screening have become accessible to a larger number of research groups. The quality of support offered at large-scale facilities allows medicinal chemistry-focused or biochemistry-focused groups to supplement their research with structural biology. Preparing the experiment, analysing multiple data sets and prospecting for interesting complexes of protein and fragments require, for both newcomers and experienced users, efficient management of the project and extensive computational power for data processing and structure refinement. Here, FragMAX, a new complete platform for fragment screening at the BioMAX beamline of the MAX IV Laboratory, is described. The ways in which users are assisted in X-ray-based fragment screenings and in which the fourth-generation storage ring available at the facility is best exploited are also described.

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