The Identification of Genetically Related Bacterial Isolates Using Pulsed Field Gel Electrophoresis on Nursing Home Units: A Clinical Experience

2004 ◽  
Vol 52 (8) ◽  
pp. 1373-1377 ◽  
Author(s):  
Paul J. Drinka ◽  
Mary E. Stemper ◽  
Cathy D. Gauerke ◽  
Janice M. Miller ◽  
Kurt D. Reed
Keyword(s):  
Nursing Home ◽  
Clinical Experience ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Bacterial Isolates ◽  
Pulsed Field

scholarly journals Genomic pattern analysis of Burkholderia mallei field isolates by pulsed-field gel electrophoresis (PFGE) discriminatory typing

2021 ◽  
Author(s):  
Shojaat Dashtipour ◽  
Keyvan Tadayon ◽  
Sajjad Yazdansetad ◽  
Nader Mosavari ◽  
Rouhollah Keshavarz
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Outbreak Detection ◽  
Pfge Type ◽  
Identification System ◽  
Suitable Model ◽  
Burkholderia Mallei ◽  
Bacterial Isolates ◽  
Pulsed Field ◽  
Field Isolates

Background and Objectives: Glanders is a serious zoonotic disease caused by Burkholderia mallei. Prevention, control, and treatment strategies of glanders are prerequisites for microbial source tracking. The present study was aimed to analyze the genomic pattern of B. mallei Iranian field isolates by pulsed-field gel electrophoresis (PFGE) typing. Materials and Methods: B. mallei isolates were aerobically cultured in nutrient broth/agar supplemented with glycerol 4% for 48 h at 37°C. API 20NE identification system was used for the biochemical characterization. Genomic DNA of bacterial isolates was extracted using OIE-recommended protocol. Molecular identification of bacterial isolates was done based on amplification of BimA and IS407-flip genes. PFGE was applied to prepare the genomic pattern of B. mallei isolates. The guinea pig was used as a suitable model for studying the histopathological characterization of B. mallei. Results: In both enzymatic digestion patterns by using Af1II and VspI, we found three different clonal types; І) PFGE type of B. mallei Razi 325 strain, ІІ) PFGE type of Tiger, Kordan, and Oshnavieh strains, and ІІІ) PFGE type of Semirom strain. B. mallei Razi 325 was categorized as unrelated strain which was belonged to the different cluster differing more than four bands. Conclusion: PFGE showed more discriminatory power and considerable reproducibility for molecular typing of B. mallei strains in our study. It is standardized the approaches for outbreak detection, pathogen phylogeny, molecular epidemiology, and population studies.

scholarly journals Pulsed-Field Gel Electrophoresis as a Molecular Tool for Characterizing Genomes of Certain Food-Borne Bacterial Isolates - A Review

2014 ◽  
Vol 29 ◽  
pp. 13-23 ◽  
Author(s):  
Partha Pal
Keyword(s):  
Gel Electrophoresis ◽  
Genetic Relatedness ◽  
Pulsed Field Gel Electrophoresis ◽  
Human Beings ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Typing Method ◽  
Gold Standard Method ◽  
Pulsed Field ◽  
Food Borne

The evolutionary transition from phenotypic to molecular analysis of infectious disease in bacterial epidemiology led to the search for suitable approaches to ascertain genomic relatedness or heterogeneity between bacterial clinical isolates. Pulsed-field gel electrophoresis (PFGE) technique was developed for separating and analyzing long DNA fragments of several megabases in alternating electric field. Comparison of electrophoresis profiles of restriction enzyme-digested genomic DNA from bacterial isolates has proved to be a useful epidemiological tool for genetic discrimination of bacterial strains, detection of genetic relatedness, to locate the source of outbreak and to monitor the spread of the microorganisms in endemic zones. PFGE is considered as a gold standard method for typing of bacterial isolates because of the remarkable endurance of this technique as a typing method for the last 20 years in molecular epidemiology. In this current review the pros and cons of PFGE use in current molecular microbiological research are explored in the context of determination of genome organization of certain food-borne bacterial isolates causing infectious diseases in human beings.

Diversity and Susceptibility of Enterococcus Isolated from Cattle before and after Harvest

2009 ◽  
Vol 72 (4) ◽  
pp. 766-774 ◽  
Author(s):  
W. M. FLUCKEY ◽  
G. H. LONERAGAN ◽  
R. D. WARNER ◽  
A. ECHEVERRY ◽  
M. M. BRASHEARS
Keyword(s):  
Gel Electrophoresis ◽  
Processing System ◽  
Drug Susceptibility ◽  
Pulsed Field Gel Electrophoresis ◽  
Antimicrobial Drug ◽  
Cross Contamination ◽  
Bacterial Isolates ◽  
Pulsed Field ◽  
Before And After ◽  
Culture Positive

To investigate evidence of cross-contamination and to determine patterns of antimicrobial drug susceptibility of Enterococcus isolates in a commercial cattle processing system, samples were collected from 60 cattle shipped to a commercial abattoir. Enterococcus isolates were recovered from fecal and hide samples collected immediately before shipment from a feedlot to the abattoir, from postexsanguination hide samples at the abattoir, and from carcass samples collected after hide removal (preevisceration) and in the cooler. Of the fecal samples, 53.9% were culture positive for Enterococcus. Of hide samples collected at the feedlot, 77.8% were positive for Enterococcus, significantly lower (P < 0.01) than the proportion of hides that were culture positive at the abattoir (96.1%). For preevisceration carcass samples, Enterococcus was recovered from 58.3% of carcasses. Only 8.3% of the carcasses sampled in the cooler yielded Enterococcus. Resistance among Enterococcus isolates was common regardless of the type or location of sample from which the isolate was recovered. All 279 Enterococcus isolates were resistant to at least one antimicrobial drug, and 179 (64.2%) of these isolates were resistant to at least six drugs. The most common resistance was to chloramphenicol (100% of isolates) followed by flavomycin (90.3%), lincomycin (87.8%), tylosin (78.5%), erythromycin (76.3%), tetracycline (58.9%), quinupristin-dalfopristin (47.7%), bacitracin (17.9), streptomycin (9.0%), ciprofloxacin (1.4%), linezolid (0.7%), and salinomycin (0.4%). Enterococcus isolates also were characterized using pulsed-field gel electrophoresis to evaluate molecular similarities. Similar or indistinguishable electrophoresis patterns were found among isolates recovered at the feedlot and in the plant, providing evidence that feedlot-origin bacterial isolates are being transferred from cattle to carcasses within the processing environment through cross-contamination.

Typing of sequential bacterial isolates by pulsed-field gel electrophoresis

1995 ◽  
Vol 22 (4) ◽  
pp. 309-314 ◽  
Author(s):  
Alan I. Hartstein ◽  
Ploenchan Chetchotisakd ◽  
Charles L. Phelps ◽  
Ann M. LeMonte
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Bacterial Isolates ◽  
Pulsed Field

scholarly journals Use of Pulsed-Field Gel Electrophoresis to Determine the Source of Methicillin-Resistant Staphylococcus aureus Bacteremia

2021 ◽  
Vol 13 (3) ◽  
pp. 602-610
Author(s):  
Eugene Y. H. Yeung ◽  
Ivan Gorn
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Epidemiological Studies ◽  
Pelvic Trauma ◽  
Pulsed Field Gel Electrophoresis ◽  
Bacterial Strains ◽  
Methicillin Resistant ◽  
Pulsed Field ◽  
Clinical Cases

Pulsed-field gel electrophoresis (PFGE) has historically been considered the gold standard in fingerprinting bacterial strains in epidemiological studies and outbreak investigations; little is known regarding its use in individual clinical cases. The current study detailed two clinical cases in which PFGE helped to determine the source of their methicillin-resistant Staphylococcus aureus (MRSA) bacteremia. Patient A was found to have MRSA bacteremia after trauma in her pelvic area. MRSA was also found in her groin but not in her nostril and rectum. PFGE was performed that showed variable bands of her MRSA isolates from blood and groin, suggestive of different strains of MRSA. Her MRSA bacteremia was determined to be unrelated to her pelvic trauma. Patient B was found to have MRSA bacteremia after colonoscopy. MRSA was also found in his nostril and rectum. PFGE was performed that showed variable bands of his MRSA isolates from blood and rectum but identical bands of MRSA isolates from his blood and nostril. His MRSA bacteremia was determined to be unrelated to his colonoscopy procedure. The current study demonstrates the use of PFGE to rule out the source of bacteremia in individual clinical cases.

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