Taxonomic Characterization of Vorticella fusca and Vorticella parapulchella n. sp., Two Marine Peritrichs (Ciliophora, Oligohymenophorea) from China

2006 ◽  
Vol 53 (5) ◽  
pp. 348-357 ◽  
Author(s):  
PING SUN ◽  
WEIBO SONG ◽  
JOHN CLAMP ◽  
KHALED A. S. AL-RASHEID
Keyword(s):  
Taxonomic Characterization

Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple

2018 ◽  
Vol 39 (4) ◽  
pp. 474-482
Author(s):  
Hoang Thi Le Thuong ◽  
Nguyen Quang Hao ◽  
Tran Thi Thuy
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Ph ◽  
Yeast Strains ◽  
Biological Studies ◽  
Taxonomic Characterization

Eight yeast strains (denoted as D1 to D8) were isolated from samples of natural fermented pineapple. Strain D8 showed highest alcoholic production at low pH and special aroma of pineapple has been chosen for further study. Taxonomic characterization of strain D8 using morphological, biochemical and molecular biological studies confirmed that strain D8  belong to Saccharomycetaceae family, Saccharomycetales order and Saccharomyces cerevisiae species. Therefore, we named this strain as Saccharomyces cerevisiae D8 for further study on Brandy production from pineapple. Citation: Hoang Thi Le Thuong, Nguyen Quang Hao, Tran Thi Thuy, 2017. Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple. Tap chi Sinh hoc, 39(4): 474- 482. DOI: 10.15625/0866-7160/v39n4.10864.*Corresponding author: [email protected] Received 5 December 2016, accepted 12 August 2017

scholarly journals Biochemical and Taxonomic Characterization of Novel Haloarchaeal Strains and Purification of the Recombinant Halotolerant α-Amylase Discovered in the Isolate

2020 ◽  
Vol 11 ◽  
Author(s):  
Dipesh Kumar Verma ◽  
Gunjan Vasudeva ◽  
Chandni Sidhu ◽  
Anil K. Pinnaka ◽  
Senthil E. Prasad ◽  
...  
Keyword(s):  
Taxonomic Characterization

scholarly journals Development and Validation of a Nested-PCR-Denaturing Gradient Gel Electrophoresis Method for Taxonomic Characterization of Bifidobacterial Communities

2003 ◽  
Vol 69 (11) ◽  
pp. 6380-6385 ◽  
Author(s):  
R. Temmerman ◽  
L. Masco ◽  
T. Vanhoutte ◽  
G. Huys ◽  
J. Swings
Keyword(s):  
Nested Pcr ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Temporal Changes ◽  
Rrna Gene ◽  
Specific Pcr ◽  
Gradient Gel Electrophoresis ◽  
Species Specific ◽  
Taxonomic Characterization

ABSTRACT The taxonomic characterization of a bacterial community is difficult to combine with the monitoring of its temporal changes. None of the currently available identification techniques are able to visualize a “complete” community, whereas techniques designed for analyzing bacterial ecosystems generally display limited or labor-intensive identification potential. This paper describes the optimization and validation of a nested-PCR-denaturing gradient gel electrophoresis (DGGE) approach for the species-specific analysis of bifidobacterial communities from any ecosystem. The method comprises a Bifidobacterium-specific PCR step, followed by purification of the amplicons that serve as template DNA in a second PCR step that amplifies the V3 and V6-V8 regions of the 16S rRNA gene. A mix of both amplicons is analyzed on a DGGE gel, after which the band positions are compared with a previously constructed database of reference strains. The method was validated through the analysis of four artificial mixtures, mimicking the possible bifidobacterial microbiota of the human and chicken intestine, a rumen, and the environment, and of two fecal samples. Except for the species Bifidobacterium coryneforme and B. indicum, all currently known bifidobacteria originating from various ecosystems can be identified in a highly reproducible manner. Because no further cloning and sequencing of the DGGE bands is necessary, this nested-PCR-DGGE technique can be completed within a 24-h span, allowing the species-specific monitoring of temporal changes in the bifidobacterial community.

Taxonomic characterization of members of the genus Corallococcus: Molecular divergence versus phenotypic coherency

2007 ◽  
Vol 30 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Erko Stackebrandt ◽  
Orsola Päuker ◽  
Ulrike Steiner ◽  
Peter Schumann ◽  
Bettina Sträubler ◽  
...  
Keyword(s):  
Molecular Divergence ◽  
Taxonomic Characterization

scholarly journals Taxonomic characterization of nine strains isolated from clinical and environmental specimens, and proposal of Corynebacterium tuberculostearicum sp. nov.

2004 ◽  
Vol 54 (4) ◽  
pp. 1055-1061 ◽  
Author(s):  
Carole Feurer ◽  
Dominique Clermont ◽  
François Bimet ◽  
Adina Candréa ◽  
Mary Jackson ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Fatty Acid Content ◽  
Food Samples ◽  
Cell Protein ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Taxonomic Analysis ◽  
Gene Sequence Analysis ◽  
Taxonomic Characterization

Nine unidentified Gram-positive, lipophilic corynebacteria were isolated from clinical and food samples and subjected to a polyphasic taxonomic analysis. The bacteria were distinguished from Corynebacterium species with validly published names by biochemical tests, fatty acid content and whole-cell protein analysis. Comparative 16S rRNA gene sequence analysis demonstrated unambiguously that the nine strains were related phylogenetically to the species ‘Corynebacterium tuberculostearicum’ and represented a distinct subline within the genus Corynebacterium. On the basis of both phenotypic and phylogenetic evidence, the formal description of Corynebacterium tuberculostearicum sp. nov. is proposed. The type strain of C. tuberculostearicum is Medalle XT (=LDC-20T=CIP 107291T=CCUG 45418T=ATCC 35529T).

scholarly journals A taxonomic review of pterotracheoid gastropod mollusks from California Current waters off the west coast of North America

Zoosymposia ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 147-150
Author(s):  
ROGER R. SEAPY
Keyword(s):  
North America ◽  
West Coast ◽  
Morphological Characteristics ◽  
California Current ◽  
Single Species ◽  
The West ◽  
Taxonomic Review ◽  
Gastropod Mollusks ◽  
Taxonomic Characterization

Taxonomic characterization of pterotracheoid gastropods, morphological characteristics and occurrence in California Current waters are reviewed. Single species of atlantid (Atlanta californiensis) and carinariid (Carinaria japonica) from these waters are described and illustrated.

scholarly journals Physical, physicochemical and taxonomic characterization of Psidium araça Raddi

2016 ◽  
Vol 1 (1) ◽  
pp. 106-110 ◽  
Author(s):  
Maria do Rosário Fátima Padilha ◽  
Neide Kazue Sakugawa Shinohara ◽  
Emanuella de Paula Rodrigues Pereira ◽  
Rejane Magalhães Mendonça Pimentel ◽  
Samara Alvachian Cardoso Andrade ◽  
...  
Keyword(s):  
Food Industry ◽  
Fruit Trees ◽  
Average Weight ◽  
Ph Values ◽  
In The Wild ◽  
Promising Application ◽  
New Research ◽  
Agricultural Regions ◽  
Taxonomic Characterization

New research has stimulated a large reevaluation of species with regards to the use of fruits from native plants. Araçá (a small Brazilian guava-like fruit) is found scattered in the wild in Pernambuco/Brazil. There is a scarcity of detailed studies on such regional fruit trees, especially the Psidium araçá Raddi species, whereas there is an enormous possibility to explore the potential offered by these fruits, such as in the field of gastronomy, where the search for new ingredients and exotic flavors associated with functional properties has been increasing. This article aimed to evaluate the physical, physicochemical, and taxonomic characterization of araçá obtained from different agricultural regions of Pernambuco in order to investigate the possibility of developing new products from this native fruit. The taxonomic identification confirmed that all collected material belonged to the species P. araçá Raddi. The average weight of 7.45 g/fruit was observed. With respect to pH, values between 3.17 and 3.48 were found, and the acidity as a percentage of citric acid was on the order of 0.96% to 0.99%. It was shown that the P. araçá Raddi fruit has a desirable quality for the food industry, presenting excellent conditions for the development of formulations of high commercial value and promising application in the national gastronomy.

scholarly journals Taxogenomics of the Genus Cyclobacterium: Cyclobacterium xiamenense and Cyclobacterium halophilum as Synonyms and Description of Cyclobacterium plantarum sp. nov.

2020 ◽  
Vol 8 (4) ◽  
pp. 610 ◽  
Author(s):  
Azadeh Shahinpei ◽  
Mohammad Ali Amoozegar ◽  
Leila Mirfeizi ◽  
Mahdi Moshtaghi Nikou ◽  
Antonio Ventosa ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Threshold Value ◽  
Amino Acid Identity ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Average Amino Acid Identity ◽  
Halophytic Plant ◽  
Taxonomic Characterization ◽  
Current Species

The genus Cyclobacterium belongs to the phylum Bacteroidetes and includes eight species. Our study, based on the genomic parameters in silico DNA–DNA hybridization (GGDC), average nucleotide identity (OrthoANI), and average amino acid identity (AAI), confirmed that all current species of Cyclobacterium belong to this genus and constitute a coherent phylogenomic group, but with species forming two separate branches. In addition, the genome-based analyses revealed that Cyclobacterium xiamenense and Cyclobacterium halophilum are members of the same species. Besides, we carried out a taxonomic characterization of the new strain GBPx2T, isolated from the halophytic plant Salicornia sp. Analysis of its 16S rRNA gene sequence showed the highest sequence similarity (97.5%) to Cyclobacterium lianum HY9T. Percentages of GGDC and OrthoANI between strain GBPx2T and species of the genus Cyclobacterium were lower than the threshold value for species delineation. The DNA G+C content was 43.0 mol%. The polar lipids included phosphatidylethanolamine as well as one unidentified phospholipid and four unidentified lipids, and its major cellular fatty acids were iso-C15:0 and summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH). The only quinone present was menaquinone 7. Based on a combination of phenotypic, chemotaxonomic, and phylogenomic features, the GBPx2T strain represents a novel species of the genus Cyclobacterium, for which the name Cyclobacterium plantarum sp. nov. is proposed. The type strain of Cyclobacterium plantarum is GBPx2T (= IBRC-M 10634T = LMG 28551T).

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